(Linear) (Six-base) MAPSORT of: KIBB7521.seq  Check: 4513  from: 1  to: 432



KIBB7521.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

Acc65I G'GTAC_C

Cuts at:      0     403     432 
   Size:        403      29
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     409     432 
   Size:          7     402      23

  Fragments arranged by size: 

                 402      23       7
 
AflIII A'CryG_T

Cuts at:      0     304     432 
   Size:        304     128
 
ApoI r'AATT_y

Cuts at:      0      36     432 
   Size:         36     396
 
BanI G'GyrC_C

Cuts at:      0     403     432 
   Size:        403      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     397     432 
   Size:        397      35
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     224     432 
   Size:        224     208
 
BmtI G_CTAG'C

Cuts at:      0     266     432 
   Size:        266     166
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     364     432 
   Size:        331      33      68

  Fragments arranged by size: 

                 331      68      33
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     344     432 
   Size:        344      88
 
BseYI C'CCAG_C

Cuts at:      0     164     432 
   Size:        164     268
 
BsrDI GCAATG_nn'

Cuts at:      0      88     432 
   Size:         88     344
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     184     432 
   Size:        184     248
 
EarI CTCTTCn'nnn_

Cuts at:      0     149     425     432 
   Size:        149     276       7

  Fragments arranged by size: 

                 276     149       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     432 
   Size:         19     413
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     331     364     409     432 
   Size:          7     324      33      45      23

  Fragments arranged by size: 

                 324      45      33      23       7
 
EcoRI G'AATT_C

Cuts at:      0      36     432 
   Size:         36     396
 
KpnI G_GTAC'C

Cuts at:      0     407     432 
   Size:        407      25
 
MspA1I CmG'CkG

Cuts at:      0     247     387     432 
   Size:        247     140      45

  Fragments arranged by size: 

                 247     140      45
 
NheI G'CTAG_C

Cuts at:      0     262     432 
   Size:        262     170
 
NspI r_CATG'y

Cuts at:      0     308     339     432 
   Size:        308      31      93

  Fragments arranged by size: 

                 308      93      31
 
PciI A'CATG_T

Cuts at:      0     304     432 
   Size:        304     128
 
PvuII CAG'CTG

Cuts at:      0     247     387     432 
   Size:        247     140      45

  Fragments arranged by size: 

                 247     140      45
 
SfcI C'TryA_G

Cuts at:      0     149     242     432 
   Size:        149      93     190

  Fragments arranged by size: 

                 190     149      93
 
SspI AAT'ATT

Cuts at:      0     111     432 
   Size:        111     321
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     ApoI     BanI     BbsI     BmrI     BmtI
     BpmI    BseRI    BseYI    BsrDI   BstAPI     EarI     EciI  Eco57MI
    EcoRI     KpnI   MspA1I     NheI     NspI     PciI    PvuII     SfcI
     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI