Restriction map for
pF1KB7522
(Linear) (Six-base) MAPSORT of: KIBB7522.seq Check: 3669 from: 1 to: 747
KIBB7522.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 658 724 747
Size: 203 455 66 23
Fragments arranged by size:
455 203 66 23
AflII C'TTAA_G
Cuts at: 0 595 747
Size: 595 152
AflIII A'CryG_T
Cuts at: 0 543 747
Size: 543 204
AleI CACnn'nnGTG
Cuts at: 0 49 747
Size: 49 698
AvaI C'yCGr_G
Cuts at: 0 527 747
Size: 527 220
BamHI G'GATC_C
Cuts at: 0 680 747
Size: 680 67
BanI G'GyrC_C
Cuts at: 0 31 747
Size: 31 716
BanII G_rGCy'C
Cuts at: 0 298 528 747
Size: 298 230 219
Fragments arranged by size:
298 230 219
BbeI G_GCGC'C
Cuts at: 0 35 747
Size: 35 712
BbsI GAAGACnn'nnnn_
Cuts at: 0 395 518 747
Size: 395 123 229
Fragments arranged by size:
395 229 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 747
Size: 128 619
BsaHI Gr'CG_yC
Cuts at: 0 32 747
Size: 32 715
BsiHKAI G_wGCw'C
Cuts at: 0 231 298 528 747
Size: 231 67 230 219
Fragments arranged by size:
231 230 219 67
BsmI GAATG_Cn'
Cuts at: 0 349 747
Size: 349 398
Bsp1286I G_dGCh'C
Cuts at: 0 231 298 528 747
Size: 231 67 230 219
Fragments arranged by size:
231 230 219 67
BspHI T'CATG_A
Cuts at: 0 195 747
Size: 195 552
BsrDI GCAATG_nn'
Cuts at: 0 94 747
Size: 94 653
BsrFI r'CCGG_y
Cuts at: 0 28 747
Size: 28 719
BstYI r'GATC_y
Cuts at: 0 180 680 747
Size: 180 500 67
Fragments arranged by size:
500 180 67
DrdI GACnn_nn'nnGTC
Cuts at: 0 159 747
Size: 159 588
EaeI y'GGCC_r
Cuts at: 0 2 747
Size: 2 745
EarI CTCTTCn'nnn_
Cuts at: 0 102 392 747
Size: 102 290 355
Fragments arranged by size:
355 290 102
EcoICRI GAG'CTC
Cuts at: 0 296 526 747
Size: 296 230 221
Fragments arranged by size:
296 230 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 658 724 747
Size: 203 455 66 23
Fragments arranged by size:
455 203 66 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 663 747
Size: 663 84
HaeII r_GCGC'y
Cuts at: 0 35 747
Size: 35 712
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 295 315 327 747
Size: 283 12 20 12 420
Fragments arranged by size:
420 283 20 12 12
KasI G'GCGC_C
Cuts at: 0 31 747
Size: 31 716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 747
Size: 243 504
MslI CAynn'nnrTG
Cuts at: 0 49 87 747
Size: 49 38 660
Fragments arranged by size:
660 49 38
MspA1I CmG'CkG
Cuts at: 0 355 432 747
Size: 355 77 315
Fragments arranged by size:
355 315 77
NarI GG'CG_CC
Cuts at: 0 32 747
Size: 32 715
NspI r_CATG'y
Cuts at: 0 375 547 558 747
Size: 375 172 11 189
Fragments arranged by size:
375 189 172 11
PciI A'CATG_T
Cuts at: 0 543 747
Size: 543 204
PspXI vC'TCGA_Gb
Cuts at: 0 527 747
Size: 527 220
PvuII CAG'CTG
Cuts at: 0 355 432 747
Size: 355 77 315
Fragments arranged by size:
355 315 77
SacI G_AGCT'C
Cuts at: 0 298 528 747
Size: 298 230 219
Fragments arranged by size:
298 230 219
SfoI GGC'GCC
Cuts at: 0 33 747
Size: 33 714
SmlI C'TyrA_G
Cuts at: 0 143 527 595 747
Size: 143 384 68 152
Fragments arranged by size:
384 152 143 68
SspI AAT'ATT
Cuts at: 0 76 192 747
Size: 76 116 555
Fragments arranged by size:
555 116 76
TatI w'GTAC_w
Cuts at: 0 713 747
Size: 713 34
XhoI C'TCGA_G
Cuts at: 0 527 747
Size: 527 220
Enzymes that do cut:
AcuI AflII AflIII AleI AvaI BamHI BanI BanII
BbeI BbsI BpuEI BsaHI BsiHKAI BsmI Bsp1286I BspHI
BsrDI BsrFI BstYI DrdI EaeI EarI EcoICRI Eco57MI
FalI HaeII Hin4I KasI MmeI MslI MspA1I NarI
NspI PciI PspXI PvuII SacI SfoI SmlI SspI
TatI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XmaI
XmnI ZraI