(Linear) (Six-base) MAPSORT of: KIBB7524.seq  Check: 2508  from: 1  to: 747



KIBB7524.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     747 
   Size:        133     614
 
ApoI r'AATT_y

Cuts at:      0     229     747 
   Size:        229     518
 
AvaI C'yCGr_G

Cuts at:      0     698     747 
   Size:        698      49
 
BamHI G'GATC_C

Cuts at:      0     573     747 
   Size:        573     174
 
BanII G_rGCy'C

Cuts at:      0     172     747 
   Size:        172     575
 
Bme1580I G_kGCm'C

Cuts at:      0     159     747 
   Size:        159     588
 
Bpu10I CC'TnA_GC

Cuts at:      0     166     747 
   Size:        166     581
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     747 
   Size:        316     431
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     615     747 
   Size:        615     132
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     595     625     747 
   Size:        595      30     122

  Fragments arranged by size: 

                 595     122      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     747 
   Size:        268     479
 
BsiHKAI G_wGCw'C

Cuts at:      0     172     429     747 
   Size:        172     257     318

  Fragments arranged by size: 

                 318     257     172
 
Bsp1286I G_dGCh'C

Cuts at:      0     159     172     429     747 
   Size:        159      13     257     318

  Fragments arranged by size: 

                 318     257     159      13
 
BspHI T'CATG_A

Cuts at:      0      80     747 
   Size:         80     667
 
BssSI C'ACGA_G

Cuts at:      0     422     490     747 
   Size:        422      68     257

  Fragments arranged by size: 

                 422     257      68
 
BstYI r'GATC_y

Cuts at:      0     200     573     747 
   Size:        200     373     174

  Fragments arranged by size: 

                 373     200     174
 
BtsI GCAGTG_nn'

Cuts at:      0     543     747 
   Size:        543     204
 
EaeI y'GGCC_r

Cuts at:      0     134     747 
   Size:        134     613
 
EarI CTCTTCn'nnn_

Cuts at:      0     375     747 
   Size:        375     372
 
EcoICRI GAG'CTC

Cuts at:      0     170     747 
   Size:        170     577
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     747 
   Size:        133     614
 
EcoO109I rG'GnC_Cy

Cuts at:      0      87     108     747 
   Size:         87      21     639

  Fragments arranged by size: 

                 639      87      21
 
FspI TGC'GCA

Cuts at:      0     496     747 
   Size:        496     251
 
FspAI rTGC'GCAy

Cuts at:      0     496     747 
   Size:        496     251
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     539     747 
   Size:        539     208
 
MscI TGG'CCA

Cuts at:      0     136     747 
   Size:        136     611
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     659     747 
   Size:        659      88
 
PfoI T'CCnGG_A

Cuts at:      0     203     747 
   Size:        203     544
 
PpuMI rG'GwC_Cy

Cuts at:      0      87     108     747 
   Size:         87      21     639

  Fragments arranged by size: 

                 639      87      21
 
SacI G_AGCT'C

Cuts at:      0     172     747 
   Size:        172     575
 
SapI GCTCTTCn'nnn_

Cuts at:      0     375     747 
   Size:        375     372
 
SexAI A'CCwGG_T

Cuts at:      0     605     747 
   Size:        605     142
 
SmlI C'TyrA_G

Cuts at:      0     331     747 
   Size:        331     416
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     595     627     747 
   Size:        595      32     120

  Fragments arranged by size: 

                 595     120      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     211     613     747 
   Size:        211     402     134

  Fragments arranged by size: 

                 402     211     134
 
XmnI GAAnn'nnTTC

Cuts at:      0      47     747 
   Size:         47     700
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BamHI    BanII Bme1580I   Bpu10I    BpuEI
     BsaI    BsaXI     BsgI  BsiHKAI Bsp1286I    BspHI    BssSI    BstYI
     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FspI    FspAI
     MmeI     MscI    PflMI     PfoI    PpuMI     SacI     SapI    SexAI
     SmlI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI