(Linear) (Six-base) MAPSORT of: KIBB7525.seq  Check: 8155  from: 1  to: 450



KIBB7525.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     149     450 
   Size:        149     301
 
Acc65I G'GTAC_C

Cuts at:      0     202     450 
   Size:        202     248
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     450 
   Size:        338     112
 
AflIII A'CryG_T

Cuts at:      0     344     450 
   Size:        344     106
 
AhdI GACnn_n'nnGTC

Cuts at:      0      72     450 
   Size:         72     378
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     398     450 
   Size:        398      52
 
ApoI r'AATT_y

Cuts at:      0     137     450 
   Size:        137     313
 
BanI G'GyrC_C

Cuts at:      0     202     450 
   Size:        202     248
 
BmtI G_CTAG'C

Cuts at:      0     445     450 
   Size:        445       5
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     429     442     450 
   Size:        429      13       8

  Fragments arranged by size: 

                 429      13       8
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     399     450 
   Size:        399      51
 
BsaAI yAC'GTr

Cuts at:      0     345     450 
   Size:        345     105
 
BsiWI C'GTAC_G

Cuts at:      0     341     450 
   Size:        341     109
 
BspHI T'CATG_A

Cuts at:      0     365     450 
   Size:        365      85
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401     450 
   Size:        401      49
 
BsrGI T'GTAC_A

Cuts at:      0     287     450 
   Size:        287     163
 
BstYI r'GATC_y

Cuts at:      0     111     450 
   Size:        111     339
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     399     450 
   Size:        338      61      51

  Fragments arranged by size: 

                 338      61      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      16     450 
   Size:         16     434
 
EcoO109I rG'GnC_Cy

Cuts at:      0     357     450 
   Size:        357      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      82      90     114     450 
   Size:         58      24       8      24     336

  Fragments arranged by size: 

                 336      58      24      24       8
 
KpnI G_GTAC'C

Cuts at:      0     206     450 
   Size:        206     244
 
NheI G'CTAG_C

Cuts at:      0     441     450 
   Size:        441       9
 
PstI C_TGCA'G

Cuts at:      0     377     450 
   Size:        377      73
 
SfcI C'TryA_G

Cuts at:      0     146     373     450 
   Size:        146     227      77

  Fragments arranged by size: 

                 227     146      77
 
StuI AGG'CCT

Cuts at:      0     243     450 
   Size:        243     207
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      83     145     450 
   Size:         83      62     305

  Fragments arranged by size: 

                 305      83      62
 
TatI w'GTAC_w

Cuts at:      0     287     450 
   Size:        287     163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     392     424     450 
   Size:        392      32      26

  Fragments arranged by size: 

                 392      32      26
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AhdI     AlfI     ApoI     BanI
     BmtI     BplI     BpmI    BsaAI    BsiWI    BspHI    BspMI    BsrGI
    BstYI  Eco57MI    EcoNI EcoO109I    Hin4I     KpnI     NheI     PstI
     SfcI     StuI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AleI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI