(Linear) (Six-base) MAPSORT of: KIBB7527.seq  Check: 8970  from: 1  to: 465



KIBB7527.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

ApoI r'AATT_y

Cuts at:      0     270     279     465 
   Size:        270       9     186

  Fragments arranged by size: 

                 270     186       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     257     368     465 
   Size:        257     111      97

  Fragments arranged by size: 

                 257     111      97
 
Bme1580I G_kGCm'C

Cuts at:      0      83     465 
   Size:         83     382
 
BsaHI Gr'CG_yC

Cuts at:      0       4     465 
   Size:          4     461
 
BsaWI w'CCGG_w

Cuts at:      0     104     465 
   Size:        104     361
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     465 
   Size:         83     382
 
BspEI T'CCGG_A

Cuts at:      0     104     465 
   Size:        104     361
 
BspHI T'CATG_A

Cuts at:      0     377     465 
   Size:        377      88
 
Bsu36I CC'TnA_GG

Cuts at:      0     328     465 
   Size:        328     137
 
DraI TTT'AAA

Cuts at:      0     275     465 
   Size:        275     190
 
EarI CTCTTCn'nnn_

Cuts at:      0     173     465 
   Size:        173     292
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     352     465 
   Size:        352     113
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     199     231     465 
   Size:        199      32     234

  Fragments arranged by size: 

                 234     199      32
 
HincII GTy'rAC

Cuts at:      0     345     465 
   Size:        345     120
 
HindIII A'AGCT_T

Cuts at:      0     306     465 
   Size:        306     159
 
MspA1I CmG'CkG

Cuts at:      0      62     465 
   Size:         62     403
 
NspI r_CATG'y

Cuts at:      0     393     465 
   Size:        393      72
 
SapI GCTCTTCn'nnn_

Cuts at:      0     173     465 
   Size:        173     292
 
SfcI C'TryA_G

Cuts at:      0     218     465 
   Size:        218     247
 

 Enzymes that do cut:

     ApoI     BbsI Bme1580I    BsaHI    BsaWI Bsp1286I    BspEI    BspHI
   Bsu36I     DraI     EarI    EcoNI    Hin4I   HincII  HindIII   MspA1I
     NspI     SapI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI