Restriction map for
pF1KB7529
(Linear) (Six-base) MAPSORT of: KIBB7529.seq Check: 7009 from: 1 to: 489
KIBB7529.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 421 489
Size: 421 68
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 382 489
Size: 361 21 107
Fragments arranged by size:
361 107 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 279 481 489
Size: 279 202 8
Fragments arranged by size:
279 202 8
AvaI C'yCGr_G
Cuts at: 0 127 445 469 478 489
Size: 127 318 24 9 11
Fragments arranged by size:
318 127 24 11 9
BanI G'GyrC_C
Cuts at: 0 230 489
Size: 230 259
BanII G_rGCy'C
Cuts at: 0 74 489
Size: 74 415
BbeI G_GCGC'C
Cuts at: 0 234 489
Size: 234 255
BbsI GAAGACnn'nnnn_
Cuts at: 0 349 489
Size: 349 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 178 489
Size: 178 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 489
Size: 178 311
BsaHI Gr'CG_yC
Cuts at: 0 231 489
Size: 231 258
BsaWI w'CCGG_w
Cuts at: 0 422 489
Size: 422 67
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 229 370 489
Size: 112 117 141 119
Fragments arranged by size:
141 119 117 112
BseYI C'CCAG_C
Cuts at: 0 76 182 489
Size: 76 106 307
Fragments arranged by size:
307 106 76
Bsp1286I G_dGCh'C
Cuts at: 0 74 489
Size: 74 415
BspEI T'CCGG_A
Cuts at: 0 422 489
Size: 422 67
BspHI T'CATG_A
Cuts at: 0 326 489
Size: 326 163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 421 489
Size: 421 68
BsrBI CCG'CTC
Cuts at: 0 315 372 489
Size: 315 57 117
Fragments arranged by size:
315 117 57
BstEII G'GTnAC_C
Cuts at: 0 30 489
Size: 30 459
BstYI r'GATC_y
Cuts at: 0 14 489
Size: 14 475
BtsI GCAGTG_nn'
Cuts at: 0 463 489
Size: 463 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 447 489
Size: 447 42
EarI CTCTTCn'nnn_
Cuts at: 0 206 489
Size: 206 283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 361 382 489
Size: 178 183 21 107
Fragments arranged by size:
183 178 107 21
EcoO109I rG'GnC_Cy
Cuts at: 0 179 489
Size: 179 310
HaeII r_GCGC'y
Cuts at: 0 234 489
Size: 234 255
KasI G'GCGC_C
Cuts at: 0 230 489
Size: 230 259
MslI CAynn'nnrTG
Cuts at: 0 388 489
Size: 388 101
MspA1I CmG'CkG
Cuts at: 0 303 489
Size: 303 186
NarI GG'CG_CC
Cuts at: 0 231 489
Size: 231 258
PflMI CCAn_nnn'nTGG
Cuts at: 0 55 489
Size: 55 434
PfoI T'CCnGG_A
Cuts at: 0 17 155 489
Size: 17 138 334
Fragments arranged by size:
334 138 17
PspXI vC'TCGA_Gb
Cuts at: 0 469 489
Size: 469 20
PstI C_TGCA'G
Cuts at: 0 302 489
Size: 302 187
RsrII CG'GwC_CG
Cuts at: 0 425 489
Size: 425 64
SfcI C'TryA_G
Cuts at: 0 298 489
Size: 298 191
SfoI GGC'GCC
Cuts at: 0 232 489
Size: 232 257
SmlI C'TyrA_G
Cuts at: 0 469 478 489
Size: 469 9 11
Fragments arranged by size:
469 11 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 442 489
Size: 442 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 417 449 489
Size: 417 32 40
Fragments arranged by size:
417 40 32
Tth111I GACn'n_nGTC
Cuts at: 0 433 489
Size: 433 56
XhoI C'TCGA_G
Cuts at: 0 469 478 489
Size: 469 9 11
Fragments arranged by size:
469 11 9
Enzymes that do cut:
AarI AcuI AlfI AvaI BanI BanII BbeI BbsI
BmrI BpmI BsaHI BsaWI BseRI BseYI Bsp1286I BspEI
BspHI BspMI BsrBI BstEII BstYI BtsI DrdI EarI
Eco57MI EcoO109I HaeII KasI MslI MspA1I NarI PflMI
PfoI PspXI PstI RsrII SfcI SfoI SmlI TaqII
TstI Tth111I XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI XbaI XcmI XmaI XmnI
ZraI