Restriction map for
pF1KB7530
(Linear) (Six-base) MAPSORT of: KIBB7530.seq Check: 7912 from: 1 to: 765
KIBB7530.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 411 765
Size: 411 354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 765
Size: 307 458
AlwNI CAG_nnn'CTG
Cuts at: 0 351 678 765
Size: 351 327 87
Fragments arranged by size:
351 327 87
ApoI r'AATT_y
Cuts at: 0 79 765
Size: 79 686
AvaI C'yCGr_G
Cuts at: 0 70 765
Size: 70 695
BanI G'GyrC_C
Cuts at: 0 281 765
Size: 281 484
BanII G_rGCy'C
Cuts at: 0 145 467 765
Size: 145 322 298
Fragments arranged by size:
322 298 145
BbeI G_GCGC'C
Cuts at: 0 285 765
Size: 285 480
BmgBI CAC'GTC
Cuts at: 0 6 765
Size: 6 759
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 677 710 765
Size: 655 22 33 55
Fragments arranged by size:
655 55 33 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 765
Size: 301 464
BsaAI yAC'GTr
Cuts at: 0 162 765
Size: 162 603
BsaHI Gr'CG_yC
Cuts at: 0 282 765
Size: 282 483
BsaWI w'CCGG_w
Cuts at: 0 635 765
Size: 635 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 765
Size: 37 30 698
Fragments arranged by size:
698 37 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 636 765
Size: 636 129
BsiHKAI G_wGCw'C
Cuts at: 0 161 765
Size: 161 604
BsmI GAATG_Cn'
Cuts at: 0 381 765
Size: 381 384
Bsp1286I G_dGCh'C
Cuts at: 0 145 161 467 765
Size: 145 16 306 298
Fragments arranged by size:
306 298 145 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 411 765
Size: 411 354
BsrBI CCG'CTC
Cuts at: 0 276 765
Size: 276 489
BsrFI r'CCGG_y
Cuts at: 0 175 765
Size: 175 590
BstAPI GCAn_nnn'nTGC
Cuts at: 0 221 408 765
Size: 221 187 357
Fragments arranged by size:
357 221 187
BstBI TT'CG_AA
Cuts at: 0 308 765
Size: 308 457
BstXI CCAn_nnnn'nTGG
Cuts at: 0 190 243 630 765
Size: 190 53 387 135
Fragments arranged by size:
387 190 135 53
BstYI r'GATC_y
Cuts at: 0 365 675 765
Size: 365 310 90
Fragments arranged by size:
365 310 90
Bsu36I CC'TnA_GG
Cuts at: 0 370 765
Size: 370 395
BtgI C'CryG_G
Cuts at: 0 242 765
Size: 242 523
BtsI GCAGTG_nn'
Cuts at: 0 195 765
Size: 195 570
EaeI y'GGCC_r
Cuts at: 0 177 765
Size: 177 588
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 267 765
Size: 267 498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 655 677 710 765
Size: 307 348 22 33 55
Fragments arranged by size:
348 307 55 33 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 225 289 765
Size: 225 64 476
Fragments arranged by size:
476 225 64
HaeII r_GCGC'y
Cuts at: 0 172 285 765
Size: 172 113 480
Fragments arranged by size:
480 172 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 604 612 636 765
Size: 580 24 8 24 129
Fragments arranged by size:
580 129 24 24 8
HincII GTy'rAC
Cuts at: 0 297 765
Size: 297 468
KasI G'GCGC_C
Cuts at: 0 281 765
Size: 281 484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 647 765
Size: 506 141 118
Fragments arranged by size:
506 141 118
MslI CAynn'nnrTG
Cuts at: 0 241 765
Size: 241 524
NarI GG'CG_CC
Cuts at: 0 282 765
Size: 282 483
NcoI C'CATG_G
Cuts at: 0 242 765
Size: 242 523
NspI r_CATG'y
Cuts at: 0 501 765
Size: 501 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 678 765
Size: 678 87
PfoI T'CCnGG_A
Cuts at: 0 671 765
Size: 671 94
PmlI CAC'GTG
Cuts at: 0 162 765
Size: 162 603
PstI C_TGCA'G
Cuts at: 0 140 404 765
Size: 140 264 361
Fragments arranged by size:
361 264 140
SbfI CC_TGCA'GG
Cuts at: 0 404 765
Size: 404 361
ScaI AGT'ACT
Cuts at: 0 40 540 765
Size: 40 500 225
Fragments arranged by size:
500 225 40
SfcI C'TryA_G
Cuts at: 0 136 400 435 765
Size: 136 264 35 330
Fragments arranged by size:
330 264 136 35
SfoI GGC'GCC
Cuts at: 0 283 765
Size: 283 482
SmlI C'TyrA_G
Cuts at: 0 316 765
Size: 316 449
SphI G_CATG'C
Cuts at: 0 501 765
Size: 501 264
StyI C'CwwG_G
Cuts at: 0 242 356 765
Size: 242 114 409
Fragments arranged by size:
409 242 114
TatI w'GTAC_w
Cuts at: 0 38 538 765
Size: 38 500 227
Fragments arranged by size:
500 227 38
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 187 765
Size: 187 578
Enzymes that do cut:
AarI AcuI AlwNI ApoI AvaI BanI BanII BbeI
BmgBI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtsI EaeI EciI Eco57MI
EcoNI HaeII Hin4I HincII KasI MmeI MslI NarI
NcoI NspI PflMI PfoI PmlI PstI SbfI ScaI
SfcI SfoI SmlI SphI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI Bpu10I BsaI BsaBI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstEII
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EarI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI MspA1I
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI XmnI ZraI