Restriction map for
pF1KB7532
(Linear) (Six-base) MAPSORT of: KIBB7532.seq Check: 1176 from: 1 to: 768
KIBB7532.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 141 768
Size: 141 627
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 768
Size: 634 134
AflIII A'CryG_T
Cuts at: 0 362 768
Size: 362 406
AleI CACnn'nnGTG
Cuts at: 0 184 397 768
Size: 184 213 371
Fragments arranged by size:
371 213 184
AlwNI CAG_nnn'CTG
Cuts at: 0 133 168 294 768
Size: 133 35 126 474
Fragments arranged by size:
474 133 126 35
ApaI G_GGCC'C
Cuts at: 0 407 488 502 608 768
Size: 407 81 14 106 160
Fragments arranged by size:
407 160 106 81 14
BanI G'GyrC_C
Cuts at: 0 513 768
Size: 513 255
BanII G_rGCy'C
Cuts at: 0 407 488 502 608 768
Size: 407 81 14 106 160
Fragments arranged by size:
407 160 106 81 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 668 768
Size: 668 100
BbvCI CC'TCA_GC
Cuts at: 0 94 539 768
Size: 94 445 229
Fragments arranged by size:
445 229 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 715 749 768
Size: 715 34 19
Fragments arranged by size:
715 34 19
Bme1580I G_kGCm'C
Cuts at: 0 329 385 407 488 502 608 768
Size: 329 56 22 81 14 106 160
Fragments arranged by size:
329 160 106 81 56 22 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 603 768
Size: 603 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 768
Size: 514 254
Bpu10I CC'TnA_GC
Cuts at: 0 94 539 768
Size: 94 445 229
Fragments arranged by size:
445 229 94
BsaHI Gr'CG_yC
Cuts at: 0 62 728 768
Size: 62 666 40
Fragments arranged by size:
666 62 40
BsaWI w'CCGG_w
Cuts at: 0 24 768
Size: 24 744
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 250 399 768
Size: 111 139 149 369
Fragments arranged by size:
369 149 139 111
BseYI C'CCAG_C
Cuts at: 0 36 266 385 591 768
Size: 36 230 119 206 177
Fragments arranged by size:
230 206 177 119 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 391 768
Size: 119 272 377
Fragments arranged by size:
377 272 119
BsiHKAI G_wGCw'C
Cuts at: 0 509 768
Size: 509 259
Bsp1286I G_dGCh'C
Cuts at: 0 329 385 407 488 502 509 608 768
Size: 329 56 22 81 14 7 99 160
Fragments arranged by size:
329 160 99 81 56 22 14 7
BspEI T'CCGG_A
Cuts at: 0 24 768
Size: 24 744
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 141 768
Size: 141 627
BsrFI r'CCGG_y
Cuts at: 0 253 768
Size: 253 515
BstAPI GCAn_nnn'nTGC
Cuts at: 0 133 768
Size: 133 635
BtgI C'CryG_G
Cuts at: 0 301 532 768
Size: 301 231 236
Fragments arranged by size:
301 236 231
BtsI GCAGTG_nn'
Cuts at: 0 126 233 485 768
Size: 126 107 252 283
Fragments arranged by size:
283 252 126 107
EaeI y'GGCC_r
Cuts at: 0 49 278 768
Size: 49 229 490
Fragments arranged by size:
490 229 49
EarI CTCTTCn'nnn_
Cuts at: 0 666 710 768
Size: 666 44 58
Fragments arranged by size:
666 58 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 422 456 768
Size: 422 34 312
Fragments arranged by size:
422 312 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 634 768
Size: 514 120 134
Fragments arranged by size:
514 134 120
EcoO109I rG'GnC_Cy
Cuts at: 0 190 403 404 484 485 498 605 768
Size: 190 213 1 80 1 13 107 163
Fragments arranged by size:
213 190 163 107 80 13 1 1
HincII GTy'rAC
Cuts at: 0 419 689 768
Size: 419 270 79
Fragments arranged by size:
419 270 79
MslI CAynn'nnrTG
Cuts at: 0 184 367 397 768
Size: 184 183 30 371
Fragments arranged by size:
371 184 183 30
MspA1I CmG'CkG
Cuts at: 0 344 358 492 768
Size: 344 14 134 276
Fragments arranged by size:
344 276 134 14
NaeI GCC'GGC
Cuts at: 0 255 768
Size: 255 513
NcoI C'CATG_G
Cuts at: 0 301 768
Size: 301 467
NgoMIV G'CCGG_C
Cuts at: 0 253 768
Size: 253 515
NspI r_CATG'y
Cuts at: 0 366 768
Size: 366 402
PciI A'CATG_T
Cuts at: 0 362 768
Size: 362 406
PpuMI rG'GwC_Cy
Cuts at: 0 190 768
Size: 190 578
PspOMI G'GGCC_C
Cuts at: 0 403 484 498 604 768
Size: 403 81 14 106 164
Fragments arranged by size:
403 164 106 81 14
PstI C_TGCA'G
Cuts at: 0 228 290 575 768
Size: 228 62 285 193
Fragments arranged by size:
285 228 193 62
PvuII CAG'CTG
Cuts at: 0 344 358 768
Size: 344 14 410
Fragments arranged by size:
410 344 14
SapI GCTCTTCn'nnn_
Cuts at: 0 666 768
Size: 666 102
SfcI C'TryA_G
Cuts at: 0 224 286 571 768
Size: 224 62 285 197
Fragments arranged by size:
285 224 197 62
StyI C'CwwG_G
Cuts at: 0 301 768
Size: 301 467
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 174 206 768
Size: 174 32 562
Fragments arranged by size:
562 174 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 118 393 477 768
Size: 118 275 84 291
Fragments arranged by size:
291 275 118 84
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI BanI BanII
BbsI BbvCI BcgI Bme1580I BmrI BpmI Bpu10I BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI
BsrFI BstAPI BtgI BtsI EaeI EarI EciI Eco57MI
EcoO109I HincII MslI MspA1I NaeI NcoI NgoMIV NspI
PciI PpuMI PspOMI PstI PvuII SapI SfcI StyI
TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI