(Linear) (Six-base) MAPSORT of: KIBB7535.seq  Check: 5581  from: 1  to: 516



KIBB7535.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     516 
   Size:        326     190
 
AleI CACnn'nnGTG

Cuts at:      0      97     516 
   Size:         97     419
 
ApaI G_GGCC'C

Cuts at:      0     308     516 
   Size:        308     208
 
BanII G_rGCy'C

Cuts at:      0     308     516 
   Size:        308     208
 
Bme1580I G_kGCm'C

Cuts at:      0     308     516 
   Size:        308     208
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     516 
   Size:         28     488
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22      96     516 
   Size:         22      74     420

  Fragments arranged by size: 

                 420      74      22
 
BsiHKAI G_wGCw'C

Cuts at:      0      26     516 
   Size:         26     490
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     308     516 
   Size:         26     282     208

  Fragments arranged by size: 

                 282     208      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     118     516 
   Size:        118     398
 
BstYI r'GATC_y

Cuts at:      0     425     516 
   Size:        425      91
 
DraI TTT'AAA

Cuts at:      0     240     516 
   Size:        240     276
 
EaeI y'GGCC_r

Cuts at:      0     232     516 
   Size:        232     284
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     295     516 
   Size:         94     201     221

  Fragments arranged by size: 

                 221     201      94
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     516 
   Size:        326     190
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     446     516 
   Size:        107     339      70

  Fragments arranged by size: 

                 339     107      70
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     144     516 
   Size:        144     372
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     179     211     320     349     352     381     516 
   Size:        179      32     109      29       3      29     135

  Fragments arranged by size: 

                 179     135     109      32      29      29       3
 
HincII GTy'rAC

Cuts at:      0     276     516 
   Size:        276     240
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     392     516 
   Size:        264     128     124

  Fragments arranged by size: 

                 264     128     124
 
MslI CAynn'nnrTG

Cuts at:      0      97     516 
   Size:         97     419
 
PpuMI rG'GwC_Cy

Cuts at:      0     107     446     516 
   Size:        107     339      70

  Fragments arranged by size: 

                 339     107      70
 
PspOMI G'GGCC_C

Cuts at:      0     304     516 
   Size:        304     212
 
SanDI GG'GwC_CC

Cuts at:      0     446     516 
   Size:        446      70
 
SapI GCTCTTCn'nnn_

Cuts at:      0      94     516 
   Size:         94     422
 
SspI AAT'ATT

Cuts at:      0     159     516 
   Size:        159     357
 
TatI w'GTAC_w

Cuts at:      0     212     516 
   Size:        212     304
 
XmnI GAAnn'nnTTC

Cuts at:      0      88     516 
   Size:         88     428
 

 Enzymes that do cut:

     AcuI     AleI     ApaI    BanII Bme1580I    BsaXI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BstYI     DraI     EaeI     EarI  Eco57MI EcoO109I
     FalI    Hin4I   HincII     MmeI     MslI    PpuMI   PspOMI    SanDI
     SapI     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI