Restriction map for
pF1KB7536
(Linear) (Six-base) MAPSORT of: KIBB7536.seq Check: 8775 from: 1 to: 771
KIBB7536.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AatII G_ACGT'C
Cuts at: 0 706 771
Size: 706 65
Acc65I G'GTAC_C
Cuts at: 0 390 771
Size: 390 381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 460 771
Size: 460 311
AlwNI CAG_nnn'CTG
Cuts at: 0 405 771
Size: 405 366
ApaI G_GGCC'C
Cuts at: 0 289 367 771
Size: 289 78 404
Fragments arranged by size:
404 289 78
ApaLI G'TGCA_C
Cuts at: 0 589 771
Size: 589 182
AvaI C'yCGr_G
Cuts at: 0 523 771
Size: 523 248
BanI G'GyrC_C
Cuts at: 0 133 145 163 276 319 390 485 771
Size: 133 12 18 113 43 71 95 286
Fragments arranged by size:
286 133 113 95 71 43 18 12
BanII G_rGCy'C
Cuts at: 0 289 367 771
Size: 289 78 404
Fragments arranged by size:
404 289 78
BbeI G_GCGC'C
Cuts at: 0 137 149 167 280 323 771
Size: 137 12 18 113 43 448
Fragments arranged by size:
448 137 113 43 18 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 771
Size: 93 678
Bme1580I G_kGCm'C
Cuts at: 0 289 367 427 565 593 771
Size: 289 78 60 138 28 178
Fragments arranged by size:
289 178 138 78 60 28
BmgBI CAC'GTC
Cuts at: 0 558 771
Size: 558 213
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 771
Size: 535 236
BsaAI yAC'GTr
Cuts at: 0 267 771
Size: 267 504
BsaHI Gr'CG_yC
Cuts at: 0 134 146 164 277 320 346 703 718
Size: 134 12 18 113 43 26 357 15
Cuts at: 718 771
Size: 53
Fragments arranged by size:
357 134 113 53 43 26 18 15
12
BsaWI w'CCGG_w
Cuts at: 0 723 771
Size: 723 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 604 634 771
Size: 532 30 42 30 137
Fragments arranged by size:
532 137 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 616 685 771
Size: 141 475 69 86
Fragments arranged by size:
475 141 86 69
BseYI C'CCAG_C
Cuts at: 0 315 323 771
Size: 315 8 448
Fragments arranged by size:
448 315 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 247 289 448 771
Size: 97 150 42 159 323
Fragments arranged by size:
323 159 150 97 42
BsiEI CG_ry'CG
Cuts at: 0 46 212 771
Size: 46 166 559
Fragments arranged by size:
559 166 46
BsiHKAI G_wGCw'C
Cuts at: 0 334 593 771
Size: 334 259 178
Fragments arranged by size:
334 259 178
Bsp1286I G_dGCh'C
Cuts at: 0 289 334 367 427 565 593 771
Size: 289 45 33 60 138 28 178
Fragments arranged by size:
289 178 138 60 45 33 28
BsrFI r'CCGG_y
Cuts at: 0 69 771
Size: 69 702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 402 771
Size: 402 369
BtgI C'CryG_G
Cuts at: 0 182 212 281 771
Size: 182 30 69 490
Fragments arranged by size:
490 182 69 30
BtsI GCAGTG_nn'
Cuts at: 0 334 771
Size: 334 437
DrdI GACnn_nn'nnGTC
Cuts at: 0 708 771
Size: 708 63
EaeI y'GGCC_r
Cuts at: 0 43 209 771
Size: 43 166 562
Fragments arranged by size:
562 166 43
EagI C'GGCC_G
Cuts at: 0 43 209 771
Size: 43 166 562
Fragments arranged by size:
562 166 43
EarI CTCTTCn'nnn_
Cuts at: 0 538 724 731 771
Size: 538 186 7 40
Fragments arranged by size:
538 186 40 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 489 771
Size: 489 282
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 598 771
Size: 535 63 173
Fragments arranged by size:
535 173 63
EcoO109I rG'GnC_Cy
Cuts at: 0 363 364 771
Size: 363 1 407
Fragments arranged by size:
407 363 1
HaeII r_GCGC'y
Cuts at: 0 137 149 167 280 323 771
Size: 137 12 18 113 43 448
Fragments arranged by size:
448 137 113 43 18 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 745 771
Size: 745 26
KasI G'GCGC_C
Cuts at: 0 133 145 163 276 319 771
Size: 133 12 18 113 43 452
Fragments arranged by size:
452 133 113 43 18 12
KpnI G_GTAC'C
Cuts at: 0 394 771
Size: 394 377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 771
Size: 656 115
MslI CAynn'nnrTG
Cuts at: 0 418 771
Size: 418 353
MspA1I CmG'CkG
Cuts at: 0 140 214 221 261 315 462 576 771
Size: 140 74 7 40 54 147 114 195
Fragments arranged by size:
195 147 140 114 74 54 40 7
NarI GG'CG_CC
Cuts at: 0 134 146 164 277 320 771
Size: 134 12 18 113 43 451
Fragments arranged by size:
451 134 113 43 18 12
NspI r_CATG'y
Cuts at: 0 15 771
Size: 15 756
PfoI T'CCnGG_A
Cuts at: 0 536 771
Size: 536 235
PmlI CAC'GTG
Cuts at: 0 267 771
Size: 267 504
PspOMI G'GGCC_C
Cuts at: 0 285 363 771
Size: 285 78 408
Fragments arranged by size:
408 285 78
PstI C_TGCA'G
Cuts at: 0 449 771
Size: 449 322
PvuII CAG'CTG
Cuts at: 0 261 462 576 771
Size: 261 201 114 195
Fragments arranged by size:
261 201 195 114
SacII CC_GC'GG
Cuts at: 0 215 771
Size: 215 556
SfcI C'TryA_G
Cuts at: 0 445 771
Size: 445 326
SfoI GGC'GCC
Cuts at: 0 135 147 165 278 321 771
Size: 135 12 18 113 43 450
Fragments arranged by size:
450 135 113 43 18 12
SmlI C'TyrA_G
Cuts at: 0 523 771
Size: 523 248
Tth111I GACn'n_nGTC
Cuts at: 0 705 771
Size: 705 66
XhoI C'TCGA_G
Cuts at: 0 523 771
Size: 523 248
XmnI GAAnn'nnTTC
Cuts at: 0 532 771
Size: 532 239
ZraI GAC'GTC
Cuts at: 0 704 771
Size: 704 67
Enzymes that do cut:
AatII Acc65I AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BbsI Bme1580I BmgBI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BsrFI BstAPI BtgI BtsI DrdI EaeI EagI EarI
EciI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI
MslI MspA1I NarI NspI PfoI PmlI PspOMI PstI
PvuII SacII SfcI SfoI SmlI Tth111I XhoI XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoICRI
Eco57MI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
XbaI XcmI XmaI