Restriction map for
pF1KB7537
(Linear) (Six-base) MAPSORT of: KIBB7537.seq Check: 8019 from: 1 to: 519
KIBB7537.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AatII G_ACGT'C
Cuts at: 0 176 519
Size: 176 343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 519
Size: 70 449
AflII C'TTAA_G
Cuts at: 0 106 519
Size: 106 413
AlwNI CAG_nnn'CTG
Cuts at: 0 96 207 275 519
Size: 96 111 68 244
Fragments arranged by size:
244 111 96 68
AscI GG'CGCG_CC
Cuts at: 0 283 519
Size: 283 236
BanI G'GyrC_C
Cuts at: 0 468 519
Size: 468 51
BanII G_rGCy'C
Cuts at: 0 116 326 455 519
Size: 116 210 129 64
Fragments arranged by size:
210 129 116 64
BglI GCCn_nnn'nGGC
Cuts at: 0 280 519
Size: 280 239
Bme1580I G_kGCm'C
Cuts at: 0 473 519
Size: 473 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 340 367 519
Size: 82 258 27 152
Fragments arranged by size:
258 152 82 27
Bpu10I CC'TnA_GC
Cuts at: 0 97 519
Size: 97 422
BsaHI Gr'CG_yC
Cuts at: 0 173 519
Size: 173 346
BsaWI w'CCGG_w
Cuts at: 0 465 519
Size: 465 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 519
Size: 340 30 149
Fragments arranged by size:
340 149 30
BseYI C'CCAG_C
Cuts at: 0 129 397 429 519
Size: 129 268 32 90
Fragments arranged by size:
268 129 90 32
BsiEI CG_ry'CG
Cuts at: 0 514 519
Size: 514 5
BsiHKAI G_wGCw'C
Cuts at: 0 353 519
Size: 353 166
Bsp1286I G_dGCh'C
Cuts at: 0 116 326 353 455 473 519
Size: 116 210 27 102 18 46
Fragments arranged by size:
210 116 102 46 27 18
BsrBI CCG'CTC
Cuts at: 0 367 463 519
Size: 367 96 56
Fragments arranged by size:
367 96 56
BsrFI r'CCGG_y
Cuts at: 0 71 519
Size: 71 448
BssHII G'CGCG_C
Cuts at: 0 75 77 79 283 335 337 393 519
Size: 75 2 2 204 52 2 56 126
Fragments arranged by size:
204 126 75 56 52 2 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 207 519
Size: 207 312
BtgI C'CryG_G
Cuts at: 0 227 514 519
Size: 227 287 5
Fragments arranged by size:
287 227 5
EaeI y'GGCC_r
Cuts at: 0 368 511 519
Size: 368 143 8
Fragments arranged by size:
368 143 8
EagI C'GGCC_G
Cuts at: 0 511 519
Size: 511 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 375 519
Size: 46 329 144
Fragments arranged by size:
329 144 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 82 340 367 519
Size: 70 12 258 27 152
Fragments arranged by size:
258 152 70 27 12
FspI TGC'GCA
Cuts at: 0 40 519
Size: 40 479
HaeII r_GCGC'y
Cuts at: 0 72 238 275 377 436 519
Size: 72 166 37 102 59 83
Fragments arranged by size:
166 102 83 72 59 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 519
Size: 442 32 45
Fragments arranged by size:
442 45 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 519
Size: 170 349
MspA1I CmG'CkG
Cuts at: 0 57 126 519
Size: 57 69 393
Fragments arranged by size:
393 69 57
NaeI GCC'GGC
Cuts at: 0 73 519
Size: 73 446
NgoMIV G'CCGG_C
Cuts at: 0 71 519
Size: 71 448
PfoI T'CCnGG_A
Cuts at: 0 176 317 519
Size: 176 141 202
Fragments arranged by size:
202 176 141
PstI C_TGCA'G
Cuts at: 0 203 519
Size: 203 316
SbfI CC_TGCA'GG
Cuts at: 0 203 519
Size: 203 316
SfcI C'TryA_G
Cuts at: 0 199 267 519
Size: 199 68 252
Fragments arranged by size:
252 199 68
SgrAI Cr'CCGG_yG
Cuts at: 0 71 519
Size: 71 448
SmlI C'TyrA_G
Cuts at: 0 106 519
Size: 106 413
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 340 372 519
Size: 340 32 147
Fragments arranged by size:
340 147 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 519
Size: 378 141
ZraI GAC'GTC
Cuts at: 0 174 519
Size: 174 345
Enzymes that do cut:
AatII AcuI AflII AlwNI AscI BanI BanII BglI
Bme1580I BpmI Bpu10I BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BsrBI BsrFI BssHII BstAPI BtgI EaeI
EagI EciI Eco57MI FspI HaeII Hin4I MmeI MspA1I
NaeI NgoMIV PfoI PstI SbfI SfcI SgrAI SmlI
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BseRI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI