Restriction map for
pF1KB7538
(Linear) (Six-base) MAPSORT of: KIBB7538.seq Check: 6372 from: 1 to: 774
KIBB7538.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 616 774
Size: 616 158
ApoI r'AATT_y
Cuts at: 0 69 774
Size: 69 705
AvaI C'yCGr_G
Cuts at: 0 180 774
Size: 180 594
BanI G'GyrC_C
Cuts at: 0 412 458 774
Size: 412 46 316
Fragments arranged by size:
412 316 46
BanII G_rGCy'C
Cuts at: 0 144 163 476 774
Size: 144 19 313 298
Fragments arranged by size:
313 298 144 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 22 774
Size: 22 752
Bme1580I G_kGCm'C
Cuts at: 0 415 774
Size: 415 359
BmrI ACTGGGnnnn_n'
Cuts at: 0 750 774
Size: 750 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 499 774
Size: 120 379 275
Fragments arranged by size:
379 275 120
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 390 502 774
Size: 390 112 272
Fragments arranged by size:
390 272 112
BseYI C'CCAG_C
Cuts at: 0 476 774
Size: 476 298
BsiHKAI G_wGCw'C
Cuts at: 0 144 774
Size: 144 630
BsmBI CGTCTCn'nnnn_
Cuts at: 0 254 774
Size: 254 520
Bsp1286I G_dGCh'C
Cuts at: 0 144 163 415 476 774
Size: 144 19 252 61 298
Fragments arranged by size:
298 252 144 61 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 770 774
Size: 620 150 4
Fragments arranged by size:
620 150 4
BsrBI CCG'CTC
Cuts at: 0 454 774
Size: 454 320
BssSI C'ACGA_G
Cuts at: 0 653 774
Size: 653 121
BstXI CCAn_nnnn'nTGG
Cuts at: 0 236 484 586 740 774
Size: 236 248 102 154 34
Fragments arranged by size:
248 236 154 102 34
BstYI r'GATC_y
Cuts at: 0 658 774
Size: 658 116
Bsu36I CC'TnA_GG
Cuts at: 0 542 774
Size: 542 232
BtgI C'CryG_G
Cuts at: 0 253 326 774
Size: 253 73 448
Fragments arranged by size:
448 253 73
ClaI AT'CG_AT
Cuts at: 0 756 774
Size: 756 18
EaeI y'GGCC_r
Cuts at: 0 576 774
Size: 576 198
EarI CTCTTCn'nnn_
Cuts at: 0 610 774
Size: 610 164
EcoICRI GAG'CTC
Cuts at: 0 142 774
Size: 142 632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 499 774
Size: 120 379 275
Fragments arranged by size:
379 275 120
EcoO109I rG'GnC_Cy
Cuts at: 0 365 774
Size: 365 409
EcoRI G'AATT_C
Cuts at: 0 69 774
Size: 69 705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 774
Size: 208 32 534
Fragments arranged by size:
534 208 32
MscI TGG'CCA
Cuts at: 0 578 774
Size: 578 196
MslI CAynn'nnrTG
Cuts at: 0 331 774
Size: 331 443
NcoI C'CATG_G
Cuts at: 0 253 326 774
Size: 253 73 448
Fragments arranged by size:
448 253 73
NspI r_CATG'y
Cuts at: 0 225 316 774
Size: 225 91 458
Fragments arranged by size:
458 225 91
PspXI vC'TCGA_Gb
Cuts at: 0 180 774
Size: 180 594
PstI C_TGCA'G
Cuts at: 0 767 774
Size: 767 7
SacI G_AGCT'C
Cuts at: 0 144 774
Size: 144 630
SapI GCTCTTCn'nnn_
Cuts at: 0 610 774
Size: 610 164
SexAI A'CCwGG_T
Cuts at: 0 461 774
Size: 461 313
SfcI C'TryA_G
Cuts at: 0 763 774
Size: 763 11
SmlI C'TyrA_G
Cuts at: 0 180 774
Size: 180 594
StyI C'CwwG_G
Cuts at: 0 99 253 326 774
Size: 99 154 73 448
Fragments arranged by size:
448 154 99 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 764 774
Size: 764 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 737 774
Size: 737 37
XhoI C'TCGA_G
Cuts at: 0 180 774
Size: 180 594
XmnI GAAnn'nnTTC
Cuts at: 0 340 774
Size: 340 434
Enzymes that do cut:
AlfI ApoI AvaI BanI BanII BbsI Bme1580I BmrI
BpmI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BssSI BstXI BstYI Bsu36I BtgI ClaI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I MscI MslI NcoI
NspI PspXI PstI SacI SapI SexAI SfcI SmlI
StyI TaqII XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
BtgZI BtsI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XmaI ZraI