(Linear) (Six-base) MAPSORT of: KIBB7539.seq  Check: 6292  from: 1  to: 531



KIBB7539.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     531 
   Size:        190     341
 
AleI CACnn'nnGTG

Cuts at:      0     400     531 
   Size:        400     131
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     525     531 
   Size:        525       6
 
ApaI G_GGCC'C

Cuts at:      0      48     531 
   Size:         48     483
 
ApoI r'AATT_y

Cuts at:      0     370     531 
   Size:        370     161
 
AvaI C'yCGr_G

Cuts at:      0      47     531 
   Size:         47     484
 
BanI G'GyrC_C

Cuts at:      0     494     531 
   Size:        494      37
 
BanII G_rGCy'C

Cuts at:      0      48     269     531 
   Size:         48     221     262

  Fragments arranged by size: 

                 262     221      48
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     269     303     531 
   Size:        269      34     228

  Fragments arranged by size: 

                 269     228      34
 
Bme1580I G_kGCm'C

Cuts at:      0      48     497     531 
   Size:         48     449      34

  Fragments arranged by size: 

                 449      48      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      51     531 
   Size:         51     480
 
BsaBI GATnn'nnATC

Cuts at:      0     200     531 
   Size:        200     331
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     378     531 
   Size:        378     153
 
BsmI GAATG_Cn'

Cuts at:      0     514     531 
   Size:        514      17
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     149     531 
   Size:        149     382
 
Bsp1286I G_dGCh'C

Cuts at:      0      48     269     497     531 
   Size:         48     221     228      34

  Fragments arranged by size: 

                 228     221      48      34
 
BsrDI GCAATG_nn'

Cuts at:      0      15     245     292     531 
   Size:         15     230      47     239

  Fragments arranged by size: 

                 239     230      47      15
 
BssSI C'ACGA_G

Cuts at:      0     323     531 
   Size:        323     208
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      14     531 
   Size:         14     517
 
BtgI C'CryG_G

Cuts at:      0      72      93     531 
   Size:         72      21     438

  Fragments arranged by size: 

                 438      72      21
 
EaeI y'GGCC_r

Cuts at:      0      36     240     531 
   Size:         36     204     291

  Fragments arranged by size: 

                 291     204      36
 
EarI CTCTTCn'nnn_

Cuts at:      0     152     531 
   Size:        152     379
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     531 
   Size:        190     341
 
EcoRI G'AATT_C

Cuts at:      0     370     531 
   Size:        370     161
 
HincII GTy'rAC

Cuts at:      0     468     531 
   Size:        468      63
 
MfeI C'AATT_G

Cuts at:      0     515     531 
   Size:        515      16
 
MscI TGG'CCA

Cuts at:      0      38     242     531 
   Size:         38     204     289

  Fragments arranged by size: 

                 289     204      38
 
MslI CAynn'nnrTG

Cuts at:      0     341     400     507     531 
   Size:        341      59     107      24

  Fragments arranged by size: 

                 341     107      59      24
 
MspA1I CmG'CkG

Cuts at:      0     479     531 
   Size:        479      52
 
PspOMI G'GGCC_C

Cuts at:      0      44     531 
   Size:         44     487
 
PstI C_TGCA'G

Cuts at:      0     451     531 
   Size:        451      80
 
SfcI C'TryA_G

Cuts at:      0     411     447     524     531 
   Size:        411      36      77       7

  Fragments arranged by size: 

                 411      77      36       7
 
SmaI CCC'GGG

Cuts at:      0      49     531 
   Size:         49     482
 
StyI C'CwwG_G

Cuts at:      0     191     251     531 
   Size:        191      60     280

  Fragments arranged by size: 

                 280     191      60
 
XmaI C'CCGG_G

Cuts at:      0      47     531 
   Size:         47     484
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     ApaI     ApoI     AvaI     BanI    BanII
     BcgI Bme1580I     BmrI    BsaBI     BsgI     BsmI    BsmBI Bsp1286I
    BsrDI    BssSI   BstAPI     BtgI     EaeI     EarI  Eco57MI    EcoRI
   HincII     MfeI     MscI     MslI   MspA1I   PspOMI     PstI     SfcI
     SmaI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI