Restriction map for
pF1KB7541
(Linear) (Six-base) MAPSORT of: KIBB7541.seq Check: 2225 from: 1 to: 534
KIBB7541.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 449 534
Size: 249 200 85
Fragments arranged by size:
249 200 85
AgeI A'CCGG_T
Cuts at: 0 218 534
Size: 218 316
AlwNI CAG_nnn'CTG
Cuts at: 0 162 534
Size: 162 372
ApaI G_GGCC'C
Cuts at: 0 44 56 534
Size: 44 12 478
Fragments arranged by size:
478 44 12
AvaI C'yCGr_G
Cuts at: 0 37 74 534
Size: 37 37 460
Fragments arranged by size:
460 37 37
BamHI G'GATC_C
Cuts at: 0 318 534
Size: 318 216
BanI G'GyrC_C
Cuts at: 0 33 534
Size: 33 501
BanII G_rGCy'C
Cuts at: 0 44 56 534
Size: 44 12 478
Fragments arranged by size:
478 44 12
BbeI G_GCGC'C
Cuts at: 0 37 534
Size: 37 497
BglI GCCn_nnn'nGGC
Cuts at: 0 520 534
Size: 520 14
BglII A'GATC_T
Cuts at: 0 149 534
Size: 149 385
BlpI GC'TnA_GC
Cuts at: 0 62 534
Size: 62 472
Bme1580I G_kGCm'C
Cuts at: 0 44 56 534
Size: 44 12 478
Fragments arranged by size:
478 44 12
BsaHI Gr'CG_yC
Cuts at: 0 34 91 534
Size: 34 57 443
Fragments arranged by size:
443 57 34
BsaWI w'CCGG_w
Cuts at: 0 218 534
Size: 218 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 534
Size: 142 392
Bsp1286I G_dGCh'C
Cuts at: 0 44 56 534
Size: 44 12 478
Fragments arranged by size:
478 44 12
BsrDI GCAATG_nn'
Cuts at: 0 303 530 534
Size: 303 227 4
Fragments arranged by size:
303 227 4
BsrFI r'CCGG_y
Cuts at: 0 218 534
Size: 218 316
BstYI r'GATC_y
Cuts at: 0 149 318 534
Size: 149 169 216
Fragments arranged by size:
216 169 149
Bsu36I CC'TnA_GG
Cuts at: 0 163 534
Size: 163 371
BtgI C'CryG_G
Cuts at: 0 143 534
Size: 143 391
EaeI y'GGCC_r
Cuts at: 0 17 534
Size: 17 517
EarI CTCTTCn'nnn_
Cuts at: 0 268 404 534
Size: 268 136 130
Fragments arranged by size:
268 136 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 449 534
Size: 249 200 85
Fragments arranged by size:
249 200 85
EcoO109I rG'GnC_Cy
Cuts at: 0 41 53 534
Size: 41 12 481
Fragments arranged by size:
481 41 12
HaeII r_GCGC'y
Cuts at: 0 37 534
Size: 37 497
HincII GTy'rAC
Cuts at: 0 84 224 534
Size: 84 140 310
Fragments arranged by size:
310 140 84
HpaI GTT'AAC
Cuts at: 0 224 534
Size: 224 310
KasI G'GCGC_C
Cuts at: 0 33 534
Size: 33 501
MspA1I CmG'CkG
Cuts at: 0 177 465 534
Size: 177 288 69
Fragments arranged by size:
288 177 69
NarI GG'CG_CC
Cuts at: 0 34 534
Size: 34 500
NcoI C'CATG_G
Cuts at: 0 143 534
Size: 143 391
NdeI CA'TA_TG
Cuts at: 0 348 534
Size: 348 186
NspI r_CATG'y
Cuts at: 0 174 534
Size: 174 360
PspOMI G'GGCC_C
Cuts at: 0 40 52 534
Size: 40 12 482
Fragments arranged by size:
482 40 12
PvuII CAG'CTG
Cuts at: 0 177 465 534
Size: 177 288 69
Fragments arranged by size:
288 177 69
SfcI C'TryA_G
Cuts at: 0 232 534
Size: 232 302
SfoI GGC'GCC
Cuts at: 0 35 534
Size: 35 499
SmaI CCC'GGG
Cuts at: 0 39 76 534
Size: 39 37 458
Fragments arranged by size:
458 39 37
SrfI GCCC'GGGC
Cuts at: 0 39 534
Size: 39 495
StyI C'CwwG_G
Cuts at: 0 143 534
Size: 143 391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 534
Size: 12 522
XmaI C'CCGG_G
Cuts at: 0 37 74 534
Size: 37 37 460
Fragments arranged by size:
460 37 37
Enzymes that do cut:
AcuI AgeI AlwNI ApaI AvaI BamHI BanI BanII
BbeI BglI BglII BlpI Bme1580I BsaHI BsaWI BsgI
Bsp1286I BsrDI BsrFI BstYI Bsu36I BtgI EaeI EarI
Eco57MI EcoO109I HaeII HincII HpaI KasI MspA1I NarI
NcoI NdeI NspI PspOMI PvuII SfcI SfoI SmaI
SrfI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII KpnI MfeI MluI MmeI MscI MslI
NaeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI