Restriction map for
pF1KB7542
(Linear) (Six-base) MAPSORT of: KIBB7542.seq Check: 2560 from: 1 to: 774
KIBB7542.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 653 774
Size: 653 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 670 774
Size: 352 318 104
Fragments arranged by size:
352 318 104
AflIII A'CryG_T
Cuts at: 0 122 209 774
Size: 122 87 565
Fragments arranged by size:
565 122 87
AleI CACnn'nnGTG
Cuts at: 0 544 774
Size: 544 230
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 287 319 774
Size: 287 32 455
Fragments arranged by size:
455 287 32
ApoI r'AATT_y
Cuts at: 0 145 774
Size: 145 629
BamHI G'GATC_C
Cuts at: 0 231 774
Size: 231 543
BanI G'GyrC_C
Cuts at: 0 25 774
Size: 25 749
BanII G_rGCy'C
Cuts at: 0 527 774
Size: 527 247
BbeI G_GCGC'C
Cuts at: 0 29 774
Size: 29 745
BbvCI CC'TCA_GC
Cuts at: 0 86 749 774
Size: 86 663 25
Fragments arranged by size:
663 86 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 453 487 774
Size: 453 34 287
Fragments arranged by size:
453 287 34
BclI T'GATC_A
Cuts at: 0 431 774
Size: 431 343
BlpI GC'TnA_GC
Cuts at: 0 521 774
Size: 521 253
BmtI G_CTAG'C
Cuts at: 0 100 774
Size: 100 674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 774
Size: 160 614
Bpu10I CC'TnA_GC
Cuts at: 0 86 749 774
Size: 86 663 25
Fragments arranged by size:
663 86 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 607 774
Size: 607 167
BsaAI yAC'GTr
Cuts at: 0 576 774
Size: 576 198
BsaHI Gr'CG_yC
Cuts at: 0 26 774
Size: 26 748
BsaWI w'CCGG_w
Cuts at: 0 173 774
Size: 173 601
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 715 745 774
Size: 715 30 29
Fragments arranged by size:
715 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 766 774
Size: 766 8
BseYI C'CCAG_C
Cuts at: 0 270 774
Size: 270 504
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 774
Size: 217 557
BsiHKAI G_wGCw'C
Cuts at: 0 527 774
Size: 527 247
Bsp1286I G_dGCh'C
Cuts at: 0 527 774
Size: 527 247
BspEI T'CCGG_A
Cuts at: 0 173 774
Size: 173 601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 653 774
Size: 653 121
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 774
Size: 228 546
BstYI r'GATC_y
Cuts at: 0 170 231 774
Size: 170 61 543
Fragments arranged by size:
543 170 61
BtgI C'CryG_G
Cuts at: 0 9 542 774
Size: 9 533 232
Fragments arranged by size:
533 232 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 15 774
Size: 15 759
DraIII CAC_nnn'GTG
Cuts at: 0 543 774
Size: 543 231
EarI CTCTTCn'nnn_
Cuts at: 0 721 774
Size: 721 53
EcoICRI GAG'CTC
Cuts at: 0 525 774
Size: 525 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 352 670 774
Size: 160 192 318 104
Fragments arranged by size:
318 192 160 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 774
Size: 649 125
EcoO109I rG'GnC_Cy
Cuts at: 0 681 774
Size: 681 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 619 774
Size: 619 155
HaeII r_GCGC'y
Cuts at: 0 29 331 774
Size: 29 302 443
Fragments arranged by size:
443 302 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 715 747 774
Size: 62 32 621 32 27
Fragments arranged by size:
621 62 32 32 27
KasI G'GCGC_C
Cuts at: 0 25 774
Size: 25 749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 774
Size: 98 676
MslI CAynn'nnrTG
Cuts at: 0 226 341 484 544 774
Size: 226 115 143 60 230
Fragments arranged by size:
230 226 143 115 60
MspA1I CmG'CkG
Cuts at: 0 11 237 520 555 748 774
Size: 11 226 283 35 193 26
Fragments arranged by size:
283 226 193 35 26 11
NarI GG'CG_CC
Cuts at: 0 26 774
Size: 26 748
NheI G'CTAG_C
Cuts at: 0 96 774
Size: 96 678
NspI r_CATG'y
Cuts at: 0 126 213 774
Size: 126 87 561
Fragments arranged by size:
561 126 87
PasI CC'CwG_GG
Cuts at: 0 685 774
Size: 685 89
PciI A'CATG_T
Cuts at: 0 122 209 774
Size: 122 87 565
Fragments arranged by size:
565 122 87
PfoI T'CCnGG_A
Cuts at: 0 203 774
Size: 203 571
PstI C_TGCA'G
Cuts at: 0 554 774
Size: 554 220
PvuII CAG'CTG
Cuts at: 0 520 555 748 774
Size: 520 35 193 26
Fragments arranged by size:
520 193 35 26
SacI G_AGCT'C
Cuts at: 0 527 774
Size: 527 247
SacII CC_GC'GG
Cuts at: 0 12 774
Size: 12 762
SfcI C'TryA_G
Cuts at: 0 550 774
Size: 550 224
SfoI GGC'GCC
Cuts at: 0 27 774
Size: 27 747
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 631 774
Size: 631 143
Enzymes that do cut:
AarI AcuI AflIII AleI AloI ApoI BamHI BanI
BanII BbeI BbvCI BcgI BclI BlpI BmtI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BstXI BstYI BtgI
BtgZI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MmeI MslI MspA1I NarI NheI
NspI PasI PciI PfoI PstI PvuII SacI SacII
SfcI SfoI TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbsI BciVI BfrBI BglI BglII Bme1580I
BmgBI BmrI BplI BpuEI BsaBI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EaeI EagI EciI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NaeI NcoI NdeI NgoMIV NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI