(Linear) (Six-base) MAPSORT of: KIBB7543.seq  Check: 7086  from: 1  to: 546



KIBB7543.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     257     546 
   Size:        257     289
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     546 
   Size:         78     468
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     422     546 
   Size:        422     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0     513     523     546 
   Size:        513      10      23

  Fragments arranged by size: 

                 513      23      10
 
ApoI r'AATT_y

Cuts at:      0     454     546 
   Size:        454      92
 
BanII G_rGCy'C

Cuts at:      0     396     546 
   Size:        396     150
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     538     546 
   Size:        538       8
 
BfrBI ATG'CAT

Cuts at:      0     409     546 
   Size:        409     137
 
BglI GCCn_nnn'nGGC

Cuts at:      0     149     546 
   Size:        149     397
 
BlpI GC'TnA_GC

Cuts at:      0      61     546 
   Size:         61     485
 
Bme1580I G_kGCm'C

Cuts at:      0      17     546 
   Size:         17     529
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     394     546 
   Size:        181     213     152

  Fragments arranged by size: 

                 213     181     152
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     479     546 
   Size:        479      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     305     335     546 
   Size:        305      30     211

  Fragments arranged by size: 

                 305     211      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     400     546 
   Size:         58     342     146

  Fragments arranged by size: 

                 342     146      58
 
BseYI C'CCAG_C

Cuts at:      0     395     546 
   Size:        395     151
 
BsiEI CG_ry'CG

Cuts at:      0     153     546 
   Size:        153     393
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     396     546 
   Size:         17     379     150

  Fragments arranged by size: 

                 379     150      17
 
BsrBI CCG'CTC

Cuts at:      0     155     546 
   Size:        155     391
 
BsrFI r'CCGG_y

Cuts at:      0     101     546 
   Size:        101     445
 
BssSI C'ACGA_G

Cuts at:      0     109     546 
   Size:        109     437
 
EaeI y'GGCC_r

Cuts at:      0     150     173     209     546 
   Size:        150      23      36     337

  Fragments arranged by size: 

                 337     150      36      23
 
EagI C'GGCC_G

Cuts at:      0     150     546 
   Size:        150     396
 
EarI CTCTTCn'nnn_

Cuts at:      0     425     546 
   Size:        425     121
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     181     394     546 
   Size:         78     103     213     152

  Fragments arranged by size: 

                 213     152     103      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171     239     271     546 
   Size:        139      32      68      32     275

  Fragments arranged by size: 

                 275     139      68      32      32
 
HincII GTy'rAC

Cuts at:      0     258     546 
   Size:        258     288
 
MscI TGG'CCA

Cuts at:      0     175     546 
   Size:        175     371
 
MspA1I CmG'CkG

Cuts at:      0     215     510     546 
   Size:        215     295      36

  Fragments arranged by size: 

                 295     215      36
 
NsiI A_TGCA'T

Cuts at:      0     411     546 
   Size:        411     135
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     354     546 
   Size:        354     192
 
PvuII CAG'CTG

Cuts at:      0     215     510     546 
   Size:        215     295      36

  Fragments arranged by size: 

                 295     215      36
 
SalI G'TCGA_C

Cuts at:      0     256     546 
   Size:        256     290
 
StuI AGG'CCT

Cuts at:      0     424     546 
   Size:        424     122
 
TatI w'GTAC_w

Cuts at:      0      83     546 
   Size:         83     463
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     355     546 
   Size:        355     191
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI    BanII     BbsI    BfrBI
     BglI     BlpI Bme1580I     BpmI     BsaI    BsaXI    BseRI    BseYI
    BsiEI Bsp1286I    BsrBI    BsrFI    BssSI     EaeI     EagI     EarI
  Eco57MI    Hin4I   HincII     MscI   MspA1I     NsiI    PflMI    PvuII
     SalI     StuI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI