Restriction map for
pF1KB7546
(Linear) (Six-base) MAPSORT of: KIBB7546.seq Check: 2585 from: 1 to: 792
KIBB7546.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 125 792
Size: 125 667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 792
Size: 332 460
ApaI G_GGCC'C
Cuts at: 0 675 792
Size: 675 117
BanI G'GyrC_C
Cuts at: 0 195 499 792
Size: 195 304 293
Fragments arranged by size:
304 293 195
BanII G_rGCy'C
Cuts at: 0 675 792
Size: 675 117
BbeI G_GCGC'C
Cuts at: 0 199 792
Size: 199 593
BbvCI CC'TCA_GC
Cuts at: 0 490 792
Size: 490 302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 392 792
Size: 358 34 400
Fragments arranged by size:
400 358 34
Bme1580I G_kGCm'C
Cuts at: 0 407 504 675 792
Size: 407 97 171 117
Fragments arranged by size:
407 171 117 97
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 282 792
Size: 282 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 427 664 792
Size: 220 207 237 128
Fragments arranged by size:
237 220 207 128
Bpu10I CC'TnA_GC
Cuts at: 0 490 777 792
Size: 490 287 15
Fragments arranged by size:
490 287 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 792
Size: 490 302
BsaBI GATnn'nnATC
Cuts at: 0 514 792
Size: 514 278
BsaHI Gr'CG_yC
Cuts at: 0 196 792
Size: 196 596
BsaWI w'CCGG_w
Cuts at: 0 138 596 792
Size: 138 458 196
Fragments arranged by size:
458 196 138
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 330 792
Size: 330 462
BseYI C'CCAG_C
Cuts at: 0 206 299 307 419 792
Size: 206 93 8 112 373
Fragments arranged by size:
373 206 112 93 8
BsiHKAI G_wGCw'C
Cuts at: 0 726 792
Size: 726 66
Bsp1286I G_dGCh'C
Cuts at: 0 407 504 675 726 792
Size: 407 97 171 51 66
Fragments arranged by size:
407 171 97 66 51
BspEI T'CCGG_A
Cuts at: 0 138 596 792
Size: 138 458 196
Fragments arranged by size:
458 196 138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 559 674 792
Size: 125 434 115 118
Fragments arranged by size:
434 125 118 115
BsrBI CCG'CTC
Cuts at: 0 659 792
Size: 659 133
BsrFI r'CCGG_y
Cuts at: 0 321 792
Size: 321 471
BstAPI GCAn_nnn'nTGC
Cuts at: 0 473 792
Size: 473 319
BstXI CCAn_nnnn'nTGG
Cuts at: 0 218 414 792
Size: 218 196 378
Fragments arranged by size:
378 218 196
BstYI r'GATC_y
Cuts at: 0 143 438 792
Size: 143 295 354
Fragments arranged by size:
354 295 143
BtsI GCAGTG_nn'
Cuts at: 0 633 792
Size: 633 159
DrdI GACnn_nn'nnGTC
Cuts at: 0 270 792
Size: 270 522
EarI CTCTTCn'nnn_
Cuts at: 0 360 792
Size: 360 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 332 427 664 792
Size: 220 112 95 237 128
Fragments arranged by size:
237 220 128 112 95
EcoNI CCTnn'n_nnAGG
Cuts at: 0 567 792
Size: 567 225
EcoO109I rG'GnC_Cy
Cuts at: 0 572 792
Size: 572 220
HaeII r_GCGC'y
Cuts at: 0 199 792
Size: 199 593
KasI G'GCGC_C
Cuts at: 0 195 792
Size: 195 597
MfeI C'AATT_G
Cuts at: 0 212 792
Size: 212 580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 686 792
Size: 56 630 106
Fragments arranged by size:
630 106 56
MslI CAynn'nnrTG
Cuts at: 0 692 792
Size: 692 100
MspA1I CmG'CkG
Cuts at: 0 414 792
Size: 414 378
NarI GG'CG_CC
Cuts at: 0 196 792
Size: 196 596
PasI CC'CwG_GG
Cuts at: 0 248 792
Size: 248 544
PflMI CCAn_nnn'nTGG
Cuts at: 0 120 792
Size: 120 672
PfoI T'CCnGG_A
Cuts at: 0 236 792
Size: 236 556
PspOMI G'GGCC_C
Cuts at: 0 671 792
Size: 671 121
PstI C_TGCA'G
Cuts at: 0 68 640 792
Size: 68 572 152
Fragments arranged by size:
572 152 68
PvuII CAG'CTG
Cuts at: 0 414 792
Size: 414 378
SfcI C'TryA_G
Cuts at: 0 54 64 636 792
Size: 54 10 572 156
Fragments arranged by size:
572 156 54 10
SfoI GGC'GCC
Cuts at: 0 197 792
Size: 197 595
SmlI C'TyrA_G
Cuts at: 0 505 792
Size: 505 287
StuI AGG'CCT
Cuts at: 0 373 457 562 792
Size: 373 84 105 230
Fragments arranged by size:
373 230 105 84
StyI C'CwwG_G
Cuts at: 0 359 434 792
Size: 359 75 358
Fragments arranged by size:
359 358 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 487 792
Size: 487 305
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 751 783 792
Size: 751 32 9
Fragments arranged by size:
751 32 9
Tth111I GACn'n_nGTC
Cuts at: 0 187 792
Size: 187 605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 176 792
Size: 176 616
XmnI GAAnn'nnTTC
Cuts at: 0 562 619 792
Size: 562 57 173
Fragments arranged by size:
562 173 57
Enzymes that do cut:
AarI AcuI ApaI BanI BanII BbeI BbvCI BcgI
Bme1580I BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI
BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BstAPI BstXI BstYI BtsI DrdI EarI Eco57MI EcoNI
EcoO109I HaeII KasI MfeI MmeI MslI MspA1I NarI
PasI PflMI PfoI PspOMI PstI PvuII SfcI SfoI
SmlI StuI StyI TaqII TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BsaI BsaAI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EaeI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XhoI XmaI ZraI