(Linear) (Six-base) MAPSORT of: KIBB7547.seq  Check: 502  from: 1  to: 555



KIBB7547.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     151     555 
   Size:        151     404
 
AccI GT'mk_AC

Cuts at:      0     177     555 
   Size:        177     378
 
AflIII A'CryG_T

Cuts at:      0     180     555 
   Size:        180     375
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     159     555 
   Size:        159     396
 
AlwNI CAG_nnn'CTG

Cuts at:      0     131     555 
   Size:        131     424
 
ApoI r'AATT_y

Cuts at:      0     531     555 
   Size:        531      24
 
AseI AT'TA_AT

Cuts at:      0     112     555 
   Size:        112     443
 
AvaI C'yCGr_G

Cuts at:      0      31     555 
   Size:         31     524
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     333     422     452     555 
   Size:        333      89      30     103

  Fragments arranged by size: 

                 333     103      89      30
 
BlpI GC'TnA_GC

Cuts at:      0      26     555 
   Size:         26     529
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     191     555 
   Size:        191     364
 
BseYI C'CCAG_C

Cuts at:      0       7     555 
   Size:          7     548
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     151     555 
   Size:        151     404
 
BstBI TT'CG_AA

Cuts at:      0     253     555 
   Size:        253     302
 
ClaI AT'CG_AT

Cuts at:      0     404     555 
   Size:        404     151
 
EcoO109I rG'GnC_Cy

Cuts at:      0     128     494     555 
   Size:        128     366      61

  Fragments arranged by size: 

                 366     128      61
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     308     356     555 
   Size:        308      48     199

  Fragments arranged by size: 

                 308     199      48
 
HaeII r_GCGC'y

Cuts at:      0      26     555 
   Size:         26     529
 
MfeI C'AATT_G

Cuts at:      0      65     555 
   Size:         65     490
 
NspI r_CATG'y

Cuts at:      0     184     555 
   Size:        184     371
 
PasI CC'CwG_GG

Cuts at:      0     131     555 
   Size:        131     424
 
PciI A'CATG_T

Cuts at:      0     180     555 
   Size:        180     375
 
PpuMI rG'GwC_Cy

Cuts at:      0     494     555 
   Size:        494      61
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     327     359     555 
   Size:        327      32     196

  Fragments arranged by size: 

                 327     196      32
 
PstI C_TGCA'G

Cuts at:      0     148     555 
   Size:        148     407
 
SfcI C'TryA_G

Cuts at:      0     144     555 
   Size:        144     411
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     245     555 
   Size:        245     310
 
XbaI T'CTAG_A

Cuts at:      0     438     555 
   Size:        438     117
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AlfI    AlwNI     ApoI     AseI     AvaI
     BbsI     BlpI     BmrI    BseYI    BspMI    BstBI     ClaI EcoO109I
     FalI    HaeII     MfeI     NspI     PasI     PciI    PpuMI     PsrI
     PstI     SfcI    TaqII     XbaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI