(Linear) (Six-base) MAPSORT of: KIBB7549.seq  Check: 4030  from: 1  to: 579



KIBB7549.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     579 
   Size:        268     311
 
AflIII A'CryG_T

Cuts at:      0      11     579 
   Size:         11     568
 
AhdI GACnn_n'nnGTC

Cuts at:      0     126     579 
   Size:        126     453
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      79     485     579 
   Size:         79     406      94

  Fragments arranged by size: 

                 406      94      79
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     579 
   Size:        132     447
 
AvaI C'yCGr_G

Cuts at:      0     335     579 
   Size:        335     244
 
BanI G'GyrC_C

Cuts at:      0     433     579 
   Size:        433     146
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     205     579 
   Size:        205     374
 
BglI GCCn_nnn'nGGC

Cuts at:      0     369     579 
   Size:        369     210
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     550     579 
   Size:        550      29
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     442     579 
   Size:        442     137
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     536     566     579 
   Size:        536      30      13

  Fragments arranged by size: 

                 536      30      13
 
BseYI C'CCAG_C

Cuts at:      0     204     579 
   Size:        204     375
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     339     481     579 
   Size:        292      47     142      98

  Fragments arranged by size: 

                 292     142      98      47
 
BsiHKAI G_wGCw'C

Cuts at:      0     331     579 
   Size:        331     248
 
Bsp1286I G_dGCh'C

Cuts at:      0     331     579 
   Size:        331     248
 
BspHI T'CATG_A

Cuts at:      0     230     579 
   Size:        230     349
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     264     579 
   Size:        264     315
 
BsrBI CCG'CTC

Cuts at:      0      23     579 
   Size:         23     556
 
BsrFI r'CCGG_y

Cuts at:      0     531     579 
   Size:        531      48
 
BstBI TT'CG_AA

Cuts at:      0     266     579 
   Size:        266     313
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     372     579 
   Size:        372     207
 
BstYI r'GATC_y

Cuts at:      0     464     579 
   Size:        464     115
 
EaeI y'GGCC_r

Cuts at:      0      93     579 
   Size:         93     486
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     579 
   Size:        268     311
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     449     481     579 
   Size:        112      32     305      32      98

  Fragments arranged by size: 

                 305     112      98      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     510     579 
   Size:        510      69
 
MscI TGG'CCA

Cuts at:      0      95     579 
   Size:         95     484
 
MslI CAynn'nnrTG

Cuts at:      0     364     579 
   Size:        364     215
 
NaeI GCC'GGC

Cuts at:      0     533     579 
   Size:        533      46
 
NgoMIV G'CCGG_C

Cuts at:      0     531     579 
   Size:        531      48
 
NspI r_CATG'y

Cuts at:      0     182     579 
   Size:        182     397
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     132     579 
   Size:        132     447
 
PstI C_TGCA'G

Cuts at:      0     189     579 
   Size:        189     390
 
SfcI C'TryA_G

Cuts at:      0      42     185     579 
   Size:         42     143     394

  Fragments arranged by size: 

                 394     143      42
 
SmaI CCC'GGG

Cuts at:      0     337     579 
   Size:        337     242
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     201     579 
   Size:        201     378
 
XmaI C'CCGG_G

Cuts at:      0     335     579 
   Size:        335     244
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AlfI    AlwNI     AvaI     BanI     BbsI
     BglI     BmrI     BsaI    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI    BstBI    BstXI    BstYI     EaeI
  Eco57MI    Hin4I     MmeI     MscI     MslI     NaeI   NgoMIV     NspI
    PflMI     PstI     SfcI     SmaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI