Restriction map for
pF1KB7550
(Linear) (Six-base) MAPSORT of: KIBB7550.seq Check: 539 from: 1 to: 801
KIBB7550.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 364 801
Size: 364 437
AatII G_ACGT'C
Cuts at: 0 428 801
Size: 428 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 715 801
Size: 272 443 86
Fragments arranged by size:
443 272 86
AfeI AGC'GCT
Cuts at: 0 556 801
Size: 556 245
AflIII A'CryG_T
Cuts at: 0 489 608 638 801
Size: 489 119 30 163
Fragments arranged by size:
489 163 119 30
AleI CACnn'nnGTG
Cuts at: 0 508 801
Size: 508 293
ApaI G_GGCC'C
Cuts at: 0 227 801
Size: 227 574
AvaI C'yCGr_G
Cuts at: 0 220 801
Size: 220 581
BanI G'GyrC_C
Cuts at: 0 434 801
Size: 434 367
BanII G_rGCy'C
Cuts at: 0 65 227 801
Size: 65 162 574
Fragments arranged by size:
574 162 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 260 801
Size: 260 541
BglI GCCn_nnn'nGGC
Cuts at: 0 677 801
Size: 677 124
BglII A'GATC_T
Cuts at: 0 86 801
Size: 86 715
Bme1580I G_kGCm'C
Cuts at: 0 227 801
Size: 227 574
BmgBI CAC'GTC
Cuts at: 0 492 684 801
Size: 492 192 117
Fragments arranged by size:
492 192 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 332 801
Size: 332 469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 586 801
Size: 75 511 215
Fragments arranged by size:
511 215 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 562 801
Size: 466 96 239
Fragments arranged by size:
466 239 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 281 338 801
Size: 281 57 463
Fragments arranged by size:
463 281 57
BsaAI yAC'GTr
Cuts at: 0 41 93 446 639 801
Size: 41 52 353 193 162
Fragments arranged by size:
353 193 162 52 41
BsaHI Gr'CG_yC
Cuts at: 0 425 801
Size: 425 376
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 661 801
Size: 88 573 140
Fragments arranged by size:
573 140 88
BseYI C'CCAG_C
Cuts at: 0 239 801
Size: 239 562
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 546 616 642 673 801
Size: 426 120 70 26 31 128
Fragments arranged by size:
426 128 120 70 31 26
BsiHKAI G_wGCw'C
Cuts at: 0 65 704 801
Size: 65 639 97
Fragments arranged by size:
639 97 65
BsmBI CGTCTCn'nnnn_
Cuts at: 0 39 801
Size: 39 762
Bsp1286I G_dGCh'C
Cuts at: 0 65 227 704 801
Size: 65 162 477 97
Fragments arranged by size:
477 162 97 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 364 801
Size: 364 437
BsrBI CCG'CTC
Cuts at: 0 621 801
Size: 621 180
BstAPI GCAn_nnn'nTGC
Cuts at: 0 449 801
Size: 449 352
BstEII G'GTnAC_C
Cuts at: 0 318 801
Size: 318 483
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 801
Size: 744 57
BstYI r'GATC_y
Cuts at: 0 86 584 801
Size: 86 498 217
Fragments arranged by size:
498 217 86
BtgI C'CryG_G
Cuts at: 0 291 588 749 801
Size: 291 297 161 52
Fragments arranged by size:
297 291 161 52
EaeI y'GGCC_r
Cuts at: 0 152 746 801
Size: 152 594 55
Fragments arranged by size:
594 152 55
EcoICRI GAG'CTC
Cuts at: 0 63 801
Size: 63 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 272 586 715 801
Size: 75 197 314 129 86
Fragments arranged by size:
314 197 129 86 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 781 801
Size: 781 20
EcoO109I rG'GnC_Cy
Cuts at: 0 224 801
Size: 224 577
FspI TGC'GCA
Cuts at: 0 304 727 801
Size: 304 423 74
Fragments arranged by size:
423 304 74
HaeII r_GCGC'y
Cuts at: 0 558 801
Size: 558 243
MluI A'CGCG_T
Cuts at: 0 608 801
Size: 608 193
MslI CAynn'nnrTG
Cuts at: 0 310 508 801
Size: 310 198 293
Fragments arranged by size:
310 293 198
MspA1I CmG'CkG
Cuts at: 0 174 243 720 801
Size: 174 69 477 81
Fragments arranged by size:
477 174 81 69
NcoI C'CATG_G
Cuts at: 0 291 588 801
Size: 291 297 213
Fragments arranged by size:
297 291 213
NspI r_CATG'y
Cuts at: 0 396 801
Size: 396 405
PmlI CAC'GTG
Cuts at: 0 41 801
Size: 41 760
PspOMI G'GGCC_C
Cuts at: 0 223 801
Size: 223 578
PstI C_TGCA'G
Cuts at: 0 785 801
Size: 785 16
PvuII CAG'CTG
Cuts at: 0 243 801
Size: 243 558
SacI G_AGCT'C
Cuts at: 0 65 801
Size: 65 736
SfcI C'TryA_G
Cuts at: 0 207 757 781 801
Size: 207 550 24 20
Fragments arranged by size:
550 207 24 20
SmaI CCC'GGG
Cuts at: 0 222 801
Size: 222 579
SmlI C'TyrA_G
Cuts at: 0 481 577 801
Size: 481 96 224
Fragments arranged by size:
481 224 96
SphI G_CATG'C
Cuts at: 0 396 801
Size: 396 405
StuI AGG'CCT
Cuts at: 0 376 544 801
Size: 376 168 257
Fragments arranged by size:
376 257 168
StyI C'CwwG_G
Cuts at: 0 291 324 588 801
Size: 291 33 264 213
Fragments arranged by size:
291 264 213 33
XmaI C'CCGG_G
Cuts at: 0 220 801
Size: 220 581
XmnI GAAnn'nnTTC
Cuts at: 0 376 460 801
Size: 376 84 341
Fragments arranged by size:
376 341 84
ZraI GAC'GTC
Cuts at: 0 426 801
Size: 426 375
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI ApaI AvaI
BanI BanII BbsI BglI BglII Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BstAPI BstEII BstXI
BstYI BtgI EaeI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII MluI MslI MspA1I NcoI NspI PmlI PspOMI
PstI PvuII SacI SfcI SmaI SmlI SphI StuI
StyI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI BplI Bpu10I BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoRI EcoRV FalI
FseI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI