(Linear) (Six-base) MAPSORT of: KIBB7551.seq  Check: 6010  from: 1  to: 591



KIBB7551.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     591 
   Size:        329     262
 
ApoI r'AATT_y

Cuts at:      0     257     438     591 
   Size:        257     181     153

  Fragments arranged by size: 

                 257     181     153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     485     591 
   Size:        485     106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     591 
   Size:        493      98
 
Bpu10I CC'TnA_GC

Cuts at:      0     419     591 
   Size:        419     172
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     298     591 
   Size:        298     293
 
BsiEI CG_ry'CG

Cuts at:      0       8     591 
   Size:          8     583
 
BspHI T'CATG_A

Cuts at:      0     110     591 
   Size:        110     481
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     300     591 
   Size:        300     291
 
Bsu36I CC'TnA_GG

Cuts at:      0      77     591 
   Size:         77     514
 
EaeI y'GGCC_r

Cuts at:      0       5      38     527     591 
   Size:          5      33     489      64

  Fragments arranged by size: 

                 489      64      33       5
 
EagI C'GGCC_G

Cuts at:      0       5     591 
   Size:          5     586
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     493     591 
   Size:        329     164      98

  Fragments arranged by size: 

                 329     164      98
 
HindIII A'AGCT_T

Cuts at:      0     341     591 
   Size:        341     250
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     591 
   Size:        410     181
 
MscI TGG'CCA

Cuts at:      0      40     529     591 
   Size:         40     489      62

  Fragments arranged by size: 

                 489      62      40
 
NspI r_CATG'y

Cuts at:      0     273     591 
   Size:        273     318
 
PsiI TTA'TAA

Cuts at:      0     539     591 
   Size:        539      52
 
PstI C_TGCA'G

Cuts at:      0     138     591 
   Size:        138     453
 
SfcI C'TryA_G

Cuts at:      0     134     591 
   Size:        134     457
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     297     315     591 
   Size:        297      18     276

  Fragments arranged by size: 

                 297     276      18
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI     BpmI   Bpu10I     BsaI    BsiEI    BspHI
    BstXI   Bsu36I     EaeI     EagI  Eco57MI  HindIII     MmeI     MscI
     NspI     PsiI     PstI     SfcI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI