Restriction map for
pF1KB7554
(Linear) (Six-base) MAPSORT of: KIBB7554.seq Check: 4174 from: 1 to: 810
KIBB7554.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 641 810
Size: 641 169
ApaI G_GGCC'C
Cuts at: 0 125 295 810
Size: 125 170 515
Fragments arranged by size:
515 170 125
ApoI r'AATT_y
Cuts at: 0 463 810
Size: 463 347
AvaI C'yCGr_G
Cuts at: 0 16 493 810
Size: 16 477 317
Fragments arranged by size:
477 317 16
BanI G'GyrC_C
Cuts at: 0 31 42 810
Size: 31 11 768
Fragments arranged by size:
768 31 11
BanII G_rGCy'C
Cuts at: 0 125 215 295 382 810
Size: 125 90 80 87 428
Fragments arranged by size:
428 125 90 87 80
BbeI G_GCGC'C
Cuts at: 0 35 810
Size: 35 775
BbsI GAAGACnn'nnnn_
Cuts at: 0 737 810
Size: 737 73
BclI T'GATC_A
Cuts at: 0 645 762 810
Size: 645 117 48
Fragments arranged by size:
645 117 48
BglI GCCn_nnn'nGGC
Cuts at: 0 259 810
Size: 259 551
BlpI GC'TnA_GC
Cuts at: 0 526 810
Size: 526 284
Bme1580I G_kGCm'C
Cuts at: 0 125 295 810
Size: 125 170 515
Fragments arranged by size:
515 170 125
BmrI ACTGGGnnnn_n'
Cuts at: 0 659 810
Size: 659 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 475 810
Size: 396 79 335
Fragments arranged by size:
396 335 79
BsaBI GATnn'nnATC
Cuts at: 0 761 810
Size: 761 49
BsaHI Gr'CG_yC
Cuts at: 0 32 88 810
Size: 32 56 722
Fragments arranged by size:
722 56 32
BsaWI w'CCGG_w
Cuts at: 0 194 810
Size: 194 616
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 591 810
Size: 156 435 219
Fragments arranged by size:
435 219 156
BsmBI CGTCTCn'nnnn_
Cuts at: 0 463 810
Size: 463 347
Bsp1286I G_dGCh'C
Cuts at: 0 125 215 295 382 810
Size: 125 90 80 87 428
Fragments arranged by size:
428 125 90 87 80
BsrFI r'CCGG_y
Cuts at: 0 21 810
Size: 21 789
BssSI C'ACGA_G
Cuts at: 0 181 810
Size: 181 629
BstAPI GCAn_nnn'nTGC
Cuts at: 0 339 660 810
Size: 339 321 150
Fragments arranged by size:
339 321 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 810
Size: 453 357
BtgI C'CryG_G
Cuts at: 0 35 117 310 810
Size: 35 82 193 500
Fragments arranged by size:
500 193 82 35
EaeI y'GGCC_r
Cuts at: 0 260 417 509 810
Size: 260 157 92 301
Fragments arranged by size:
301 260 157 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 237 334 479 810
Size: 237 97 145 331
Fragments arranged by size:
331 237 145 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 475 810
Size: 396 79 335
Fragments arranged by size:
396 335 79
EcoO109I rG'GnC_Cy
Cuts at: 0 291 292 810
Size: 291 1 518
Fragments arranged by size:
518 291 1
HaeII r_GCGC'y
Cuts at: 0 35 810
Size: 35 775
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 489 521 724 756 810
Size: 489 32 203 32 54
Fragments arranged by size:
489 203 54 32 32
KasI G'GCGC_C
Cuts at: 0 31 810
Size: 31 779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 810
Size: 458 352
MscI TGG'CCA
Cuts at: 0 511 810
Size: 511 299
MspA1I CmG'CkG
Cuts at: 0 48 66 119 265 312 810
Size: 48 18 53 146 47 498
Fragments arranged by size:
498 146 53 48 47 18
NaeI GCC'GGC
Cuts at: 0 23 810
Size: 23 787
NarI GG'CG_CC
Cuts at: 0 32 810
Size: 32 778
NgoMIV G'CCGG_C
Cuts at: 0 21 810
Size: 21 789
PasI CC'CwG_GG
Cuts at: 0 357 810
Size: 357 453
PspOMI G'GGCC_C
Cuts at: 0 121 291 810
Size: 121 170 519
Fragments arranged by size:
519 170 121
PstI C_TGCA'G
Cuts at: 0 524 810
Size: 524 286
SacII CC_GC'GG
Cuts at: 0 120 313 810
Size: 120 193 497
Fragments arranged by size:
497 193 120
SexAI A'CCwGG_T
Cuts at: 0 547 810
Size: 547 263
SfcI C'TryA_G
Cuts at: 0 520 810
Size: 520 290
SfoI GGC'GCC
Cuts at: 0 33 810
Size: 33 777
SmaI CCC'GGG
Cuts at: 0 18 810
Size: 18 792
StuI AGG'CCT
Cuts at: 0 396 810
Size: 396 414
XmaI C'CCGG_G
Cuts at: 0 16 810
Size: 16 794
XmnI GAAnn'nnTTC
Cuts at: 0 463 810
Size: 463 347
Enzymes that do cut:
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BclI BglI BlpI Bme1580I BmrI BpmI BsaBI BsaHI
BsaWI BseRI BsmBI Bsp1286I BsrFI BssSI BstAPI BstXI
BtgI EaeI EciI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MscI MspA1I NaeI NarI NgoMIV PasI PspOMI
PstI SacII SexAI SfcI SfoI SmaI StuI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MslI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI ZraI