(Linear) (Six-base) MAPSORT of: KIBB7561.seq  Check: 6039  from: 1  to: 621



KIBB7561.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     367     621 
   Size:        367     254
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     397     621 
   Size:        317      80     224

  Fragments arranged by size: 

                 317     224      80
 
ApoI r'AATT_y

Cuts at:      0     418     608     621 
   Size:        418     190      13

  Fragments arranged by size: 

                 418     190      13
 
BanI G'GyrC_C

Cuts at:      0     311     621 
   Size:        311     310
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134     621 
   Size:        134     487
 
BclI T'GATC_A

Cuts at:      0     120     621 
   Size:        120     501
 
BsaBI GATnn'nnATC

Cuts at:      0     170     621 
   Size:        170     451
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     514     621 
   Size:        514     107
 
BspHI T'CATG_A

Cuts at:      0      93     123     621 
   Size:         93      30     498

  Fragments arranged by size: 

                 498      93      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     241     621 
   Size:        241     380
 
EarI CTCTTCn'nnn_

Cuts at:      0     482     621 
   Size:        482     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     397     621 
   Size:        317      80     224

  Fragments arranged by size: 

                 317     224      80
 
HincII GTy'rAC

Cuts at:      0     550     621 
   Size:        550      71
 
MspA1I CmG'CkG

Cuts at:      0     526     621 
   Size:        526      95
 
PsiI TTA'TAA

Cuts at:      0     289     621 
   Size:        289     332
 
PstI C_TGCA'G

Cuts at:      0      57     621 
   Size:         57     564
 
PvuII CAG'CTG

Cuts at:      0     526     621 
   Size:        526      95
 
SapI GCTCTTCn'nnn_

Cuts at:      0     482     621 
   Size:        482     139
 
SfcI C'TryA_G

Cuts at:      0      53     470     621 
   Size:         53     417     151

  Fragments arranged by size: 

                 417     151      53
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     523     621 
   Size:        523      98
 
XmnI GAAnn'nnTTC

Cuts at:      0     254     350     621 
   Size:        254      96     271

  Fragments arranged by size: 

                 271     254      96
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BanI     BbsI     BclI    BsaBI    BseRI
    BspHI   BstAPI     EarI  Eco57MI   HincII   MspA1I     PsiI     PstI
    PvuII     SapI     SfcI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI