(Linear) (Six-base) MAPSORT of: KIBB7563.seq  Check: 8274  from: 1  to: 621



KIBB7563.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     544     621 
   Size:        544      77
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     621 
   Size:        160     461
 
ApoI r'AATT_y

Cuts at:      0     436     621 
   Size:        436     185
 
AvaI C'yCGr_G

Cuts at:      0      48     621 
   Size:         48     573
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      13     598     621 
   Size:         13     585      23

  Fragments arranged by size: 

                 585      23      13
 
BmtI G_CTAG'C

Cuts at:      0     418     621 
   Size:        418     203
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     345     621 
   Size:        345     276
 
BsaHI Gr'CG_yC

Cuts at:      0      92     621 
   Size:         92     529
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     251     621 
   Size:        251     370
 
BsiWI C'GTAC_G

Cuts at:      0      11     621 
   Size:         11     610
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      73     544     621 
   Size:         73     471      77

  Fragments arranged by size: 

                 471      77      73
 
BssSI C'ACGA_G

Cuts at:      0     497     621 
   Size:        497     124
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      65     621 
   Size:         65     556
 
BtgI C'CryG_G

Cuts at:      0     242     621 
   Size:        242     379
 
DraI TTT'AAA

Cuts at:      0     135     621 
   Size:        135     486
 
EarI CTCTTCn'nnn_

Cuts at:      0      28     314     336     470     621 
   Size:         28     286      22     134     151

  Fragments arranged by size: 

                 286     151     134      28      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     621 
   Size:        160     461
 
HindIII A'AGCT_T

Cuts at:      0     172     621 
   Size:        172     449
 
MfeI C'AATT_G

Cuts at:      0     419     621 
   Size:        419     202
 
MspA1I CmG'CkG

Cuts at:      0     193     621 
   Size:        193     428
 
NcoI C'CATG_G

Cuts at:      0     242     621 
   Size:        242     379
 
NheI G'CTAG_C

Cuts at:      0     414     621 
   Size:        414     207
 
NspI r_CATG'y

Cuts at:      0     328     621 
   Size:        328     293
 
PshAI GACnn'nnGTC

Cuts at:      0     390     621 
   Size:        390     231
 
SapI GCTCTTCn'nnn_

Cuts at:      0     314     621 
   Size:        314     307
 
SfcI C'TryA_G

Cuts at:      0     231     621 
   Size:        231     390
 
SphI G_CATG'C

Cuts at:      0     328     621 
   Size:        328     293
 
SspI AAT'ATT

Cuts at:      0     294     621 
   Size:        294     327
 
StyI C'CwwG_G

Cuts at:      0     242     621 
   Size:        242     379
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     AvaI     BbsI     BmtI     BplI    BsaHI
    BseRI    BsiWI    BspMI    BssSI   BstAPI     BtgI     DraI     EarI
  Eco57MI  HindIII     MfeI   MspA1I     NcoI     NheI     NspI    PshAI
     SapI     SfcI     SphI     SspI     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI