Restriction map for
pF1KB7564
(Linear) (Six-base) MAPSORT of: KIBB7564.seq Check: 7696 from: 1 to: 825
KIBB7564.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 601 825
Size: 505 96 224
Fragments arranged by size:
505 224 96
AfeI AGC'GCT
Cuts at: 0 559 825
Size: 559 266
AleI CACnn'nnGTG
Cuts at: 0 359 825
Size: 359 466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 626 825
Size: 626 199
ApaI G_GGCC'C
Cuts at: 0 268 670 825
Size: 268 402 155
Fragments arranged by size:
402 268 155
ApaLI G'TGCA_C
Cuts at: 0 444 825
Size: 444 381
AvaI C'yCGr_G
Cuts at: 0 130 229 244 825
Size: 130 99 15 581
Fragments arranged by size:
581 130 99 15
AvrII C'CTAG_G
Cuts at: 0 105 825
Size: 105 720
BanII G_rGCy'C
Cuts at: 0 65 251 268 670 825
Size: 65 186 17 402 155
Fragments arranged by size:
402 186 155 65 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 3 825
Size: 3 822
Bme1580I G_kGCm'C
Cuts at: 0 268 448 670 708 825
Size: 268 180 222 38 117
Fragments arranged by size:
268 222 180 117 38
BmgBI CAC'GTC
Cuts at: 0 495 825
Size: 495 330
BmrI ACTGGGnnnn_n'
Cuts at: 0 91 825
Size: 91 734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 825
Size: 228 597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 589 825
Size: 215 374 236
Fragments arranged by size:
374 236 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 825
Size: 397 428
BsaI GGTCTCn'nnnn_
Cuts at: 0 45 825
Size: 45 780
BsaAI yAC'GTr
Cuts at: 0 335 696 825
Size: 335 361 129
Fragments arranged by size:
361 335 129
BsaHI Gr'CG_yC
Cuts at: 0 257 825
Size: 257 568
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 825
Size: 670 30 125
Fragments arranged by size:
670 125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 42 212 823 825
Size: 39 3 170 611 2
Fragments arranged by size:
611 170 39 3 2
BseYI C'CCAG_C
Cuts at: 0 53 554 825
Size: 53 501 271
Fragments arranged by size:
501 271 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 619 676 825
Size: 357 262 57 149
Fragments arranged by size:
357 262 149 57
BsiEI CG_ry'CG
Cuts at: 0 302 825
Size: 302 523
BsiHKAI G_wGCw'C
Cuts at: 0 65 448 825
Size: 65 383 377
Fragments arranged by size:
383 377 65
BsmI GAATG_Cn'
Cuts at: 0 512 825
Size: 512 313
Bsp1286I G_dGCh'C
Cuts at: 0 65 251 268 448 670 708 825
Size: 65 186 17 180 222 38 117
Fragments arranged by size:
222 186 180 117 65 38 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 698 825
Size: 698 127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 396 452 536 825
Size: 396 56 84 289
Fragments arranged by size:
396 289 84 56
BtgI C'CryG_G
Cuts at: 0 591 825
Size: 591 234
EarI CTCTTCn'nnn_
Cuts at: 0 16 481 825
Size: 16 465 344
Fragments arranged by size:
465 344 16
EcoICRI GAG'CTC
Cuts at: 0 63 825
Size: 63 762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 505 589 601 825
Size: 215 290 84 12 224
Fragments arranged by size:
290 224 215 84 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 805 825
Size: 760 45 20
Fragments arranged by size:
760 45 20
EcoO109I rG'GnC_Cy
Cuts at: 0 264 265 667 681 825
Size: 264 1 402 14 144
Fragments arranged by size:
402 264 144 14 1
HaeII r_GCGC'y
Cuts at: 0 297 561 825
Size: 297 264 264
Fragments arranged by size:
297 264 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 825
Size: 299 32 494
Fragments arranged by size:
494 299 32
MslI CAynn'nnrTG
Cuts at: 0 97 359 825
Size: 97 262 466
Fragments arranged by size:
466 262 97
MspA1I CmG'CkG
Cuts at: 0 53 164 170 359 624 825
Size: 53 111 6 189 265 201
Fragments arranged by size:
265 201 189 111 53 6
NcoI C'CATG_G
Cuts at: 0 591 825
Size: 591 234
NspI r_CATG'y
Cuts at: 0 399 825
Size: 399 426
PfoI T'CCnGG_A
Cuts at: 0 65 825
Size: 65 760
PmlI CAC'GTG
Cuts at: 0 335 696 825
Size: 335 361 129
Fragments arranged by size:
361 335 129
PpuMI rG'GwC_Cy
Cuts at: 0 681 825
Size: 681 144
PspOMI G'GGCC_C
Cuts at: 0 264 666 825
Size: 264 402 159
Fragments arranged by size:
402 264 159
PstI C_TGCA'G
Cuts at: 0 392 764 825
Size: 392 372 61
Fragments arranged by size:
392 372 61
PvuII CAG'CTG
Cuts at: 0 53 359 825
Size: 53 306 466
Fragments arranged by size:
466 306 53
RsrII CG'GwC_CG
Cuts at: 0 45 825
Size: 45 780
SacI G_AGCT'C
Cuts at: 0 65 825
Size: 65 760
SexAI A'CCwGG_T
Cuts at: 0 425 825
Size: 425 400
SfcI C'TryA_G
Cuts at: 0 388 760 825
Size: 388 372 65
Fragments arranged by size:
388 372 65
SmlI C'TyrA_G
Cuts at: 0 412 825
Size: 412 413
SphI G_CATG'C
Cuts at: 0 399 825
Size: 399 426
StyI C'CwwG_G
Cuts at: 0 105 591 825
Size: 105 486 234
Fragments arranged by size:
486 234 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 568 688 825
Size: 33 535 120 137
Fragments arranged by size:
535 137 120 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 825
Size: 12 813
Enzymes that do cut:
AcuI AfeI AleI AlfI ApaI ApaLI AvaI AvrII
BanII BbsI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BstAPI BtgI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I MslI MspA1I NcoI
NspI PfoI PmlI PpuMI PspOMI PstI PvuII RsrII
SacI SexAI SfcI SmlI SphI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI AlwNI ApoI AscI AseI AsiSI BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmtI Bpu10I BsaBI BsaWI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI