(Linear) (Six-base) MAPSORT of: KIBB7565.seq  Check: 5411  from: 1  to: 627



KIBB7565.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

ApaI G_GGCC'C

Cuts at:      0     523     627 
   Size:        523     104
 
ApoI r'AATT_y

Cuts at:      0     118     627 
   Size:        118     509
 
BanII G_rGCy'C

Cuts at:      0     523     627 
   Size:        523     104
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     154     627 
   Size:        154     473
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     363     627 
   Size:        363     264
 
BlpI GC'TnA_GC

Cuts at:      0     446     627 
   Size:        446     181
 
Bme1580I G_kGCm'C

Cuts at:      0     523     627 
   Size:        523     104
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       5     627 
   Size:          5     622
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     583     586     589     592     616     627 
   Size:        583       3       3       3      24      11

  Fragments arranged by size: 

                 583      24      11       3       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     627 
   Size:         79     548
 
BsiHKAI G_wGCw'C

Cuts at:      0      80     627 
   Size:         80     547
 
BsiWI C'GTAC_G

Cuts at:      0      44     627 
   Size:         44     583
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     523     627 
   Size:         80     443     104

  Fragments arranged by size: 

                 443     104      80
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      73     627 
   Size:         73     554
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     487     627 
   Size:        487     140
 
BtgI C'CryG_G

Cuts at:      0      25     627 
   Size:         25     602
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     310     627 
   Size:        310     317
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     103     627 
   Size:        103     524
 
EarI CTCTTCn'nnn_

Cuts at:      0      68     105     602     627 
   Size:         68      37     497      25

  Fragments arranged by size: 

                 497      68      37      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     242     627 
   Size:        242     385
 
EcoO109I rG'GnC_Cy

Cuts at:      0     270     519     520     627 
   Size:        270     249       1     107

  Fragments arranged by size: 

                 270     249     107       1
 
EcoRI G'AATT_C

Cuts at:      0     118     627 
   Size:        118     509
 
MspA1I CmG'CkG

Cuts at:      0      27     627 
   Size:         27     600
 
NdeI CA'TA_TG

Cuts at:      0     429     627 
   Size:        429     198
 
PpuMI rG'GwC_Cy

Cuts at:      0     270     627 
   Size:        270     357
 
PspOMI G'GGCC_C

Cuts at:      0     519     627 
   Size:        519     108
 
SacII CC_GC'GG

Cuts at:      0      28     627 
   Size:         28     599
 
SapI GCTCTTCn'nnn_

Cuts at:      0      68     627 
   Size:         68     559
 
StyI C'CwwG_G

Cuts at:      0     491     627 
   Size:        491     136
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     376     627 
   Size:        376     251
 
Tth111I GACn'n_nGTC

Cuts at:      0     153     627 
   Size:        153     474
 

 Enzymes that do cut:

     ApaI     ApoI    BanII     BbsI    BciVI     BlpI Bme1580I     BsaI
    BseRI     BsgI  BsiHKAI    BsiWI Bsp1286I    BspMI   BstAPI     BtgI
    BtgZI     DrdI     EarI    EcoNI EcoO109I    EcoRI   MspA1I     NdeI
    PpuMI   PspOMI    SacII     SapI     StyI    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI