(Linear) (Six-base) MAPSORT of: KIBB7567.seq  Check: 2815  from: 1  to: 630



KIBB7567.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     374     469     524     630 
   Size:        158     216      95      55     106

  Fragments arranged by size: 

                 216     158     106      95      55
 
AfeI AGC'GCT

Cuts at:      0     616     630 
   Size:        616      14
 
AvaI C'yCGr_G

Cuts at:      0     365     630 
   Size:        365     265
 
BanII G_rGCy'C

Cuts at:      0      23     209     462     630 
   Size:         23     186     253     168

  Fragments arranged by size: 

                 253     186     168      23
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     576     630 
   Size:        576      54
 
BglII A'GATC_T

Cuts at:      0      96     630 
   Size:         96     534
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     175     630 
   Size:        175     455
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     477     630 
   Size:         97     380     153

  Fragments arranged by size: 

                 380     153      97
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      70     630 
   Size:         70     560
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157     630 
   Size:        157     473
 
BsiEI CG_ry'CG

Cuts at:      0       8     630 
   Size:          8     622
 
BsiHKAI G_wGCw'C

Cuts at:      0      23     209     462     630 
   Size:         23     186     253     168

  Fragments arranged by size: 

                 253     186     168      23
 
BsmI GAATG_Cn'

Cuts at:      0     311     630 
   Size:        311     319
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       4     630 
   Size:          4     626
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     209     462     630 
   Size:         23     186     253     168

  Fragments arranged by size: 

                 253     186     168      23
 
BssSI C'ACGA_G

Cuts at:      0      65     630 
   Size:         65     565
 
BstYI r'GATC_y

Cuts at:      0      96     630 
   Size:         96     534
 
EarI CTCTTCn'nnn_

Cuts at:      0     134     307     489     592     630 
   Size:        134     173     182     103      38

  Fragments arranged by size: 

                 182     173     134     103      38
 
EcoICRI GAG'CTC

Cuts at:      0      21     207     460     630 
   Size:         21     186     253     170

  Fragments arranged by size: 

                 253     186     170      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     158     374     469     477     524     630 
   Size:         97      61     216      95       8      47     106

  Fragments arranged by size: 

                 216     106      97      95      61      47       8
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      76     630 
   Size:         76     554
 
HaeII r_GCGC'y

Cuts at:      0     618     630 
   Size:        618      12
 
HincII GTy'rAC

Cuts at:      0     435     630 
   Size:        435     195
 
HindIII A'AGCT_T

Cuts at:      0      34     630 
   Size:         34     596
 
HpaI GTT'AAC

Cuts at:      0     435     630 
   Size:        435     195
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     630 
   Size:        421     209
 
MspA1I CmG'CkG

Cuts at:      0     562     630 
   Size:        562      68
 
NspI r_CATG'y

Cuts at:      0     136     630 
   Size:        136     494
 
PvuII CAG'CTG

Cuts at:      0     562     630 
   Size:        562      68
 
SacI G_AGCT'C

Cuts at:      0      23     209     462     630 
   Size:         23     186     253     168

  Fragments arranged by size: 

                 253     186     168      23
 
StyI C'CwwG_G

Cuts at:      0      80     630 
   Size:         80     550
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      22     630 
   Size:         22     608
 
TatI w'GTAC_w

Cuts at:      0     162     630 
   Size:        162     468
 

 Enzymes that do cut:

     AcuI     AfeI     AvaI    BanII    BciVI    BglII     BmrI     BpmI
     BsaI    BseRI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BssSI
    BstYI     EarI  EcoICRI  Eco57MI     FalI    HaeII   HincII  HindIII
     HpaI     MmeI   MspA1I     NspI    PvuII     SacI     StyI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiWI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI