Restriction map for
pF1KB7571
(Linear) (Six-base) MAPSORT of: KIBB7571.seq Check: 3840 from: 1 to: 615
KIBB7571.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 615
Size: 229 386
AlwNI CAG_nnn'CTG
Cuts at: 0 445 583 615
Size: 445 138 32
Fragments arranged by size:
445 138 32
ApaI G_GGCC'C
Cuts at: 0 574 615
Size: 574 41
AvaI C'yCGr_G
Cuts at: 0 116 124 567 615
Size: 116 8 443 48
Fragments arranged by size:
443 116 48 8
BanI G'GyrC_C
Cuts at: 0 99 319 615
Size: 99 220 296
Fragments arranged by size:
296 220 99
BanII G_rGCy'C
Cuts at: 0 131 514 574 615
Size: 131 383 60 41
Fragments arranged by size:
383 131 60 41
BbeI G_GCGC'C
Cuts at: 0 323 615
Size: 323 292
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 299 615
Size: 193 106 316
Fragments arranged by size:
316 193 106
BbvCI CC'TCA_GC
Cuts at: 0 576 615
Size: 576 39
Bme1580I G_kGCm'C
Cuts at: 0 574 615
Size: 574 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 615
Size: 165 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 615
Size: 247 368
Bpu10I CC'TnA_GC
Cuts at: 0 576 615
Size: 576 39
BsaHI Gr'CG_yC
Cuts at: 0 320 615
Size: 320 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 615
Size: 220 30 365
Fragments arranged by size:
365 220 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 615
Size: 472 143
BseYI C'CCAG_C
Cuts at: 0 558 615
Size: 558 57
BsiEI CG_ry'CG
Cuts at: 0 354 615
Size: 354 261
BsiHKAI G_wGCw'C
Cuts at: 0 131 615
Size: 131 484
Bsp1286I G_dGCh'C
Cuts at: 0 131 514 574 615
Size: 131 383 60 41
Fragments arranged by size:
383 131 60 41
BsrFI r'CCGG_y
Cuts at: 0 39 615
Size: 39 576
BstEII G'GTnAC_C
Cuts at: 0 165 615
Size: 165 450
BstXI CCAn_nnnn'nTGG
Cuts at: 0 307 615
Size: 307 308
BtsI GCAGTG_nn'
Cuts at: 0 220 615
Size: 220 395
EaeI y'GGCC_r
Cuts at: 0 351 615
Size: 351 264
EagI C'GGCC_G
Cuts at: 0 351 615
Size: 351 264
EarI CTCTTCn'nnn_
Cuts at: 0 68 136 449 584 615
Size: 68 68 313 135 31
Fragments arranged by size:
313 135 68 68 31
EcoICRI GAG'CTC
Cuts at: 0 129 615
Size: 129 486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 247 615
Size: 229 18 368
Fragments arranged by size:
368 229 18
EcoO109I rG'GnC_Cy
Cuts at: 0 252 571 615
Size: 252 319 44
Fragments arranged by size:
319 252 44
HaeII r_GCGC'y
Cuts at: 0 124 323 565 615
Size: 124 199 242 50
Fragments arranged by size:
242 199 124 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 448 480 595 615
Size: 412 32 4 32 115 20
Fragments arranged by size:
412 115 32 32 20 4
KasI G'GCGC_C
Cuts at: 0 319 615
Size: 319 296
MslI CAynn'nnrTG
Cuts at: 0 305 615
Size: 305 310
MspA1I CmG'CkG
Cuts at: 0 347 615
Size: 347 268
NaeI GCC'GGC
Cuts at: 0 41 615
Size: 41 574
NarI GG'CG_CC
Cuts at: 0 320 615
Size: 320 295
NgoMIV G'CCGG_C
Cuts at: 0 39 615
Size: 39 576
NspI r_CATG'y
Cuts at: 0 279 615
Size: 279 336
PfoI T'CCnGG_A
Cuts at: 0 400 615
Size: 400 215
PpuMI rG'GwC_Cy
Cuts at: 0 252 615
Size: 252 363
PspOMI G'GGCC_C
Cuts at: 0 570 615
Size: 570 45
PspXI vC'TCGA_Gb
Cuts at: 0 124 615
Size: 124 491
SacI G_AGCT'C
Cuts at: 0 131 615
Size: 131 484
SapI GCTCTTCn'nnn_
Cuts at: 0 136 615
Size: 136 479
SexAI A'CCwGG_T
Cuts at: 0 254 615
Size: 254 361
SfoI GGC'GCC
Cuts at: 0 321 615
Size: 321 294
SgrAI Cr'CCGG_yG
Cuts at: 0 39 615
Size: 39 576
SmaI CCC'GGG
Cuts at: 0 118 569 615
Size: 118 451 46
Fragments arranged by size:
451 118 46
SmlI C'TyrA_G
Cuts at: 0 124 615
Size: 124 491
StuI AGG'CCT
Cuts at: 0 157 615
Size: 157 458
TatI w'GTAC_w
Cuts at: 0 20 615
Size: 20 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 348 380 615
Size: 348 32 235
Fragments arranged by size:
348 235 32
Tth111I GACn'n_nGTC
Cuts at: 0 171 615
Size: 171 444
XhoI C'TCGA_G
Cuts at: 0 124 615
Size: 124 491
XmaI C'CCGG_G
Cuts at: 0 116 567 615
Size: 116 451 48
Fragments arranged by size:
451 116 48
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BbvCI Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BsrFI BstEII BstXI BtsI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
KasI MslI MspA1I NaeI NarI NgoMIV NspI PfoI
PpuMI PspOMI PspXI SacI SapI SexAI SfoI SgrAI
SmaI SmlI StuI TatI TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
XbaI XcmI XmnI ZraI