(Linear) (Six-base) MAPSORT of: KIBB7581.seq  Check: 5348  from: 1  to: 525



KIBB7581.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     525 
   Size:        121     404
 
AleI CACnn'nnGTG

Cuts at:      0     121     525 
   Size:        121     404
 
AlwNI CAG_nnn'CTG

Cuts at:      0      35     144     525 
   Size:         35     109     381

  Fragments arranged by size: 

                 381     109      35
 
BamHI G'GATC_C

Cuts at:      0     496     525 
   Size:        496      29
 
BanII G_rGCy'C

Cuts at:      0     481     510     525 
   Size:        481      29      15

  Fragments arranged by size: 

                 481      29      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      56     525 
   Size:         56     469
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     476     525 
   Size:        476      49
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     291     525 
   Size:        291     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     524     525 
   Size:        524       1
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     439     525 
   Size:        439      86
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     468     498     525 
   Size:        468      30      27

  Fragments arranged by size: 

                 468      30      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     304     525 
   Size:        100     204     221

  Fragments arranged by size: 

                 221     204     100
 
BseYI C'CCAG_C

Cuts at:      0     377     472     525 
   Size:        377      95      53

  Fragments arranged by size: 

                 377      95      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     423     525 
   Size:        405      18     102

  Fragments arranged by size: 

                 405     102      18
 
BsmI GAATG_Cn'

Cuts at:      0     321     525 
   Size:        321     204
 
Bsp1286I G_dGCh'C

Cuts at:      0     481     510     525 
   Size:        481      29      15

  Fragments arranged by size: 

                 481      29      15
 
BsrFI r'CCGG_y

Cuts at:      0     154     525 
   Size:        154     371
 
BstYI r'GATC_y

Cuts at:      0     496     525 
   Size:        496      29
 
BtgI C'CryG_G

Cuts at:      0     119     525 
   Size:        119     406
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     226     525 
   Size:        226     299
 
EaeI y'GGCC_r

Cuts at:      0       2     525 
   Size:          2     523
 
EarI CTCTTCn'nnn_

Cuts at:      0     437     525 
   Size:        437      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     524     525 
   Size:        121     403       1

  Fragments arranged by size: 

                 403     121       1
 
HaeII r_GCGC'y

Cuts at:      0      20     457     525 
   Size:         20     437      68

  Fragments arranged by size: 

                 437      68      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     468     500     525 
   Size:         46      32     390      32      25

  Fragments arranged by size: 

                 390      46      32      32      25
 
MslI CAynn'nnrTG

Cuts at:      0     121     525 
   Size:        121     404
 
MspA1I CmG'CkG

Cuts at:      0      32     381     525 
   Size:         32     349     144

  Fragments arranged by size: 

                 349     144      32
 
NaeI GCC'GGC

Cuts at:      0     156     525 
   Size:        156     369
 
NcoI C'CATG_G

Cuts at:      0     119     525 
   Size:        119     406
 
NgoMIV G'CCGG_C

Cuts at:      0     154     525 
   Size:        154     371
 
PfoI T'CCnGG_A

Cuts at:      0     263     525 
   Size:        263     262
 
StyI C'CwwG_G

Cuts at:      0     119     320     525 
   Size:        119     201     205

  Fragments arranged by size: 

                 205     201     119
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI    BamHI    BanII     BbsI     BmrI     BplI
     BpmI     BsaI    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I
    BsrFI    BstYI     BtgI    BtgZI     EaeI     EarI  Eco57MI    HaeII
    Hin4I     MslI   MspA1I     NaeI     NcoI   NgoMIV     PfoI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NarI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI