Restriction map for
pF1KB7584
(Linear) (Six-base) MAPSORT of: KIBB7584.seq Check: 1729 from: 1 to: 861
KIBB7584.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AfeI AGC'GCT
Cuts at: 0 132 861
Size: 132 729
AhdI GACnn_n'nnGTC
Cuts at: 0 324 861
Size: 324 537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 706 861
Size: 706 155
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 526 558 861
Size: 526 32 303
Fragments arranged by size:
526 303 32
AlwNI CAG_nnn'CTG
Cuts at: 0 306 459 768 861
Size: 306 153 309 93
Fragments arranged by size:
309 306 153 93
ApaI G_GGCC'C
Cuts at: 0 836 861
Size: 836 25
AvaI C'yCGr_G
Cuts at: 0 97 153 469 829 861
Size: 97 56 316 360 32
Fragments arranged by size:
360 316 97 56 32
BanI G'GyrC_C
Cuts at: 0 576 861
Size: 576 285
BanII G_rGCy'C
Cuts at: 0 76 836 861
Size: 76 760 25
Fragments arranged by size:
760 76 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 861
Size: 95 766
BbvCI CC'TCA_GC
Cuts at: 0 800 861
Size: 800 61
BglI GCCn_nnn'nGGC
Cuts at: 0 71 484 861
Size: 71 413 377
Fragments arranged by size:
413 377 71
BlpI GC'TnA_GC
Cuts at: 0 25 861
Size: 25 836
Bme1580I G_kGCm'C
Cuts at: 0 836 861
Size: 836 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 316 447 861
Size: 316 131 414
Fragments arranged by size:
414 316 131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 393 604 861
Size: 33 360 211 257
Fragments arranged by size:
360 257 211 33
Bpu10I CC'TnA_GC
Cuts at: 0 58 800 861
Size: 58 742 61
Fragments arranged by size:
742 61 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 700 730 861
Size: 253 30 417 30 131
Fragments arranged by size:
417 253 131 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 861
Size: 352 509
BseYI C'CCAG_C
Cuts at: 0 137 495 721 861
Size: 137 358 226 140
Fragments arranged by size:
358 226 140 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 861
Size: 799 62
BsiEI CG_ry'CG
Cuts at: 0 18 861
Size: 18 843
BsiHKAI G_wGCw'C
Cuts at: 0 590 861
Size: 590 271
Bsp1286I G_dGCh'C
Cuts at: 0 76 590 836 861
Size: 76 514 246 25
Fragments arranged by size:
514 246 76 25
BsrBI CCG'CTC
Cuts at: 0 702 861
Size: 702 159
BsrFI r'CCGG_y
Cuts at: 0 275 861
Size: 275 586
BssHII G'CGCG_C
Cuts at: 0 474 861
Size: 474 387
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 861
Size: 306 555
BstXI CCAn_nnnn'nTGG
Cuts at: 0 166 729 861
Size: 166 563 132
Fragments arranged by size:
563 166 132
BtgI C'CryG_G
Cuts at: 0 611 861
Size: 611 250
EaeI y'GGCC_r
Cuts at: 0 168 422 485 861
Size: 168 254 63 376
Fragments arranged by size:
376 254 168 63
EarI CTCTTCn'nnn_
Cuts at: 0 660 861
Size: 660 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 393 604 861
Size: 33 360 211 257
Fragments arranged by size:
360 257 211 33
EcoO109I rG'GnC_Cy
Cuts at: 0 318 833 861
Size: 318 515 28
Fragments arranged by size:
515 318 28
FspI TGC'GCA
Cuts at: 0 358 861
Size: 358 503
FspAI rTGC'GCAy
Cuts at: 0 358 861
Size: 358 503
HaeII r_GCGC'y
Cuts at: 0 134 236 438 466 861
Size: 134 102 202 28 395
Fragments arranged by size:
395 202 134 102 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 310 342 861
Size: 136 32 142 32 519
Fragments arranged by size:
519 142 136 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 861
Size: 230 631
MscI TGG'CCA
Cuts at: 0 170 861
Size: 170 691
MspA1I CmG'CkG
Cuts at: 0 306 420 495 582 690 861
Size: 306 114 75 87 108 171
Fragments arranged by size:
306 171 114 108 87 75
NcoI C'CATG_G
Cuts at: 0 611 861
Size: 611 250
PasI CC'CwG_GG
Cuts at: 0 847 861
Size: 847 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 459 749 861
Size: 459 290 112
Fragments arranged by size:
459 290 112
PpuMI rG'GwC_Cy
Cuts at: 0 318 861
Size: 318 543
PspOMI G'GGCC_C
Cuts at: 0 832 861
Size: 832 29
PstI C_TGCA'G
Cuts at: 0 434 861
Size: 434 427
PvuI CG_AT'CG
Cuts at: 0 18 861
Size: 18 843
PvuII CAG'CTG
Cuts at: 0 420 495 690 861
Size: 420 75 195 171
Fragments arranged by size:
420 195 171 75
SfcI C'TryA_G
Cuts at: 0 126 430 861
Size: 126 304 431
Fragments arranged by size:
431 304 126
SmaI CCC'GGG
Cuts at: 0 831 861
Size: 831 30
StyI C'CwwG_G
Cuts at: 0 77 611 861
Size: 77 534 250
Fragments arranged by size:
534 250 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 417 861
Size: 417 444
XmaI C'CCGG_G
Cuts at: 0 829 861
Size: 829 32
Enzymes that do cut:
AfeI AhdI AlfI AloI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BsrBI BsrFI BssHII BstAPI BstXI BtgI EaeI EarI
Eco57MI EcoO109I FspI FspAI HaeII Hin4I MmeI MscI
MspA1I NcoI PasI PflMI PpuMI PspOMI PstI PvuI
PvuII SfcI SmaI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KasI KpnI MfeI MluI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmnI ZraI