Restriction map for
pF1KB7590
(Linear) (Six-base) MAPSORT of: KIBB7590.seq Check: 5204 from: 1 to: 870
KIBB7590.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AfeI AGC'GCT
Cuts at: 0 367 870
Size: 367 503
AflIII A'CryG_T
Cuts at: 0 313 870
Size: 313 557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 240 256 870
Size: 240 16 614
Fragments arranged by size:
614 240 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 870
Size: 343 32 495
Fragments arranged by size:
495 343 32
AlwNI CAG_nnn'CTG
Cuts at: 0 851 870
Size: 851 19
ApaI G_GGCC'C
Cuts at: 0 34 209 591 870
Size: 34 175 382 279
Fragments arranged by size:
382 279 175 34
AvaI C'yCGr_G
Cuts at: 0 202 870
Size: 202 668
BanI G'GyrC_C
Cuts at: 0 13 646 870
Size: 13 633 224
Fragments arranged by size:
633 224 13
BanII G_rGCy'C
Cuts at: 0 34 209 591 870
Size: 34 175 382 279
Fragments arranged by size:
382 279 175 34
BbeI G_GCGC'C
Cuts at: 0 17 870
Size: 17 853
BbvCI CC'TCA_GC
Cuts at: 0 147 513 705 870
Size: 147 366 192 165
Fragments arranged by size:
366 192 165 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 511 545 870
Size: 511 34 325
Fragments arranged by size:
511 325 34
BglI GCCn_nnn'nGGC
Cuts at: 0 523 623 644 870
Size: 523 100 21 226
Fragments arranged by size:
523 226 100 21
Bme1580I G_kGCm'C
Cuts at: 0 34 209 584 591 649 870
Size: 34 175 375 7 58 221
Fragments arranged by size:
375 221 175 58 34 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 799 870
Size: 799 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 153 370 514 577 870
Size: 138 15 217 144 63 293
Fragments arranged by size:
293 217 144 138 63 15
Bpu10I CC'TnA_GC
Cuts at: 0 147 513 705 870
Size: 147 366 192 165
Fragments arranged by size:
366 192 165 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 71 421 482 870
Size: 71 350 61 388
Fragments arranged by size:
388 350 71 61
BsaAI yAC'GTr
Cuts at: 0 316 870
Size: 316 554
BsaHI Gr'CG_yC
Cuts at: 0 14 505 870
Size: 14 491 365
Fragments arranged by size:
491 365 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 870
Size: 343 30 497
Fragments arranged by size:
497 343 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 501 663 870
Size: 501 162 207
Fragments arranged by size:
501 207 162
BsiEI CG_ry'CG
Cuts at: 0 27 870
Size: 27 843
Bsp1286I G_dGCh'C
Cuts at: 0 34 209 584 591 649 870
Size: 34 175 375 7 58 221
Fragments arranged by size:
375 221 175 58 34 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 639 870
Size: 639 231
BsrBI CCG'CTC
Cuts at: 0 91 762 870
Size: 91 671 108
Fragments arranged by size:
671 108 91
BsrFI r'CCGG_y
Cuts at: 0 299 566 778 870
Size: 299 267 212 92
Fragments arranged by size:
299 267 212 92
BssHII G'CGCG_C
Cuts at: 0 37 870
Size: 37 833
BstEII G'GTnAC_C
Cuts at: 0 658 870
Size: 658 212
BstXI CCAn_nnnn'nTGG
Cuts at: 0 818 870
Size: 818 52
BstYI r'GATC_y
Cuts at: 0 768 870
Size: 768 102
Bsu36I CC'TnA_GG
Cuts at: 0 281 654 870
Size: 281 373 216
Fragments arranged by size:
373 281 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 20 870
Size: 20 850
BtsI GCAGTG_nn'
Cuts at: 0 222 326 718 870
Size: 222 104 392 152
Fragments arranged by size:
392 222 152 104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 116 151 870
Size: 116 35 719
Fragments arranged by size:
719 116 35
EaeI y'GGCC_r
Cuts at: 0 117 137 870
Size: 117 20 733
Fragments arranged by size:
733 117 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 61 870
Size: 61 809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 153 370 514 577 870
Size: 138 15 217 144 63 293
Fragments arranged by size:
293 217 144 138 63 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 870
Size: 280 590
EcoO109I rG'GnC_Cy
Cuts at: 0 30 31 143 285 587 588 870
Size: 30 1 112 142 302 1 282
Fragments arranged by size:
302 282 142 112 30 1 1
HaeII r_GCGC'y
Cuts at: 0 17 72 369 607 845 870
Size: 17 55 297 238 238 25
Fragments arranged by size:
297 238 238 55 25 17
KasI G'GCGC_C
Cuts at: 0 13 870
Size: 13 857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 870
Size: 563 307
MscI TGG'CCA
Cuts at: 0 119 870
Size: 119 751
MslI CAynn'nnrTG
Cuts at: 0 238 870
Size: 238 632
MspA1I CmG'CkG
Cuts at: 0 86 339 414 693 739 818 851 870
Size: 86 253 75 279 46 79 33 19
Fragments arranged by size:
279 253 86 79 75 46 33 19
NaeI GCC'GGC
Cuts at: 0 568 780 870
Size: 568 212 90
Fragments arranged by size:
568 212 90
NarI GG'CG_CC
Cuts at: 0 14 870
Size: 14 856
NgoMIV G'CCGG_C
Cuts at: 0 566 778 870
Size: 566 212 92
Fragments arranged by size:
566 212 92
PasI CC'CwG_GG
Cuts at: 0 584 870
Size: 584 286
PflMI CCAn_nnn'nTGG
Cuts at: 0 378 870
Size: 378 492
PfoI T'CCnGG_A
Cuts at: 0 764 870
Size: 764 106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 870
Size: 343 32 495
Fragments arranged by size:
495 343 32
PpuMI rG'GwC_Cy
Cuts at: 0 143 870
Size: 143 727
PspOMI G'GGCC_C
Cuts at: 0 30 205 587 870
Size: 30 175 382 283
Fragments arranged by size:
382 283 175 30
PvuII CAG'CTG
Cuts at: 0 339 414 739 818 851 870
Size: 339 75 325 79 33 19
Fragments arranged by size:
339 325 79 75 33 19
SfoI GGC'GCC
Cuts at: 0 15 870
Size: 15 855
SmaI CCC'GGG
Cuts at: 0 204 870
Size: 204 666
SrfI GCCC'GGGC
Cuts at: 0 204 870
Size: 204 666
StuI AGG'CCT
Cuts at: 0 526 626 870
Size: 526 100 244
Fragments arranged by size:
526 244 100
XmaI C'CCGG_G
Cuts at: 0 202 870
Size: 202 668
Enzymes that do cut:
AfeI AflIII AlfI AloI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BcgI BglI Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BsiEI Bsp1286I
BspMI BsrBI BsrFI BssHII BstEII BstXI BstYI Bsu36I
BtgZI BtsI CspCI EaeI EciI Eco57MI EcoNI EcoO109I
HaeII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV PasI PflMI PfoI PpiI PpuMI PspOMI PvuII
SfoI SmaI SrfI StuI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BpuEI BsaBI BsaWI BseYI BsgI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssSI BstAPI BstBI BstZ17I BtgI ClaI DraI DraIII
DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI