(Linear) (Six-base) MAPSORT of: KIBB7592.seq  Check: 5606  from: 1  to: 873



KIBB7592.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AclI AA'CG_TT

Cuts at:      0      72     873 
   Size:         72     801
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     862     873 
   Size:        730     132      11

  Fragments arranged by size: 

                 730     132      11
 
AlwNI CAG_nnn'CTG

Cuts at:      0     654     873 
   Size:        654     219
 
ApoI r'AATT_y

Cuts at:      0     159     302     494     643     873 
   Size:        159     143     192     149     230

  Fragments arranged by size: 

                 230     192     159     149     143
 
BamHI G'GATC_C

Cuts at:      0       3     873 
   Size:          3     870
 
BanII G_rGCy'C

Cuts at:      0      48     873 
   Size:         48     825
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     262     512     873 
   Size:        262     250     361

  Fragments arranged by size: 

                 361     262     250
 
BclI T'GATC_A

Cuts at:      0     165     873 
   Size:        165     708
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     854     873 
   Size:        854      19
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     873 
   Size:        322     551
 
BsaBI GATnn'nnATC

Cuts at:      0     281     873 
   Size:        281     592
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     287     675     703     873 
   Size:        287     388      28     170

  Fragments arranged by size: 

                 388     287     170      28
 
BsiHKAI G_wGCw'C

Cuts at:      0      48     873 
   Size:         48     825
 
Bsp1286I G_dGCh'C

Cuts at:      0      48     873 
   Size:         48     825
 
BsrDI GCAATG_nn'

Cuts at:      0     180     873 
   Size:        180     693
 
BstYI r'GATC_y

Cuts at:      0       3     873 
   Size:          3     870
 
BtgI C'CryG_G

Cuts at:      0     680     873 
   Size:        680     193
 
BtsI GCAGTG_nn'

Cuts at:      0     520     873 
   Size:        520     353
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     737     772     873 
   Size:        737      35     101

  Fragments arranged by size: 

                 737     101      35
 
EcoICRI GAG'CTC

Cuts at:      0      46     873 
   Size:         46     827
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     854     862     873 
   Size:        730     124       8      11

  Fragments arranged by size: 

                 730     124      11       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0     447     873 
   Size:        447     426
 
EcoRI G'AATT_C

Cuts at:      0     494     873 
   Size:        494     379
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     822     873 
   Size:        822      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     249     281     873 
   Size:         79      32     138      32     592

  Fragments arranged by size: 

                 592     138      79      32      32
 
HincII GTy'rAC

Cuts at:      0     375     873 
   Size:        375     498
 
HindIII A'AGCT_T

Cuts at:      0     194     873 
   Size:        194     679
 
MslI CAynn'nnrTG

Cuts at:      0     173     873 
   Size:        173     700
 
NcoI C'CATG_G

Cuts at:      0     680     873 
   Size:        680     193
 
SacI G_AGCT'C

Cuts at:      0      48     873 
   Size:         48     825
 
SfcI C'TryA_G

Cuts at:      0      59     671     873 
   Size:         59     612     202

  Fragments arranged by size: 

                 612     202      59
 
SmlI C'TyrA_G

Cuts at:      0     337     873 
   Size:        337     536
 
StyI C'CwwG_G

Cuts at:      0     680     873 
   Size:        680     193
 
TatI w'GTAC_w

Cuts at:      0     425     873 
   Size:        425     448
 
XmnI GAAnn'nnTTC

Cuts at:      0     644     873 
   Size:        644     229
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI    BamHI    BanII     BbsI     BclI
     BpmI    BpuEI    BsaBI    BseRI  BsiHKAI Bsp1286I    BsrDI    BstYI
     BtgI     BtsI    CspCI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
    Hin4I   HincII  HindIII     MslI     NcoI     SacI     SfcI     SmlI
     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI    EcoNI    EcoRV     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI