Restriction map for
pF1KB7600
(Linear) (Six-base) MAPSORT of: KIBB7600.seq Check: 2235 from: 1 to: 1302
KIBB7600.orf
With 182 enzymes: SgfI *
September 25, 2008 15:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 121 617 1302
Size: 121 496 685
Fragments arranged by size:
685 496 121
AatII G_ACGT'C
Cuts at: 0 668 1302
Size: 668 634
AccI GT'mk_AC
Cuts at: 0 566 1302
Size: 566 736
AgeI A'CCGG_T
Cuts at: 0 54 1302
Size: 54 1248
AhdI GACnn_n'nnGTC
Cuts at: 0 123 669 1302
Size: 123 546 633
Fragments arranged by size:
633 546 123
AleI CACnn'nnGTG
Cuts at: 0 37 1060 1302
Size: 37 1023 242
Fragments arranged by size:
1023 242 37
AlwNI CAG_nnn'CTG
Cuts at: 0 22 867 1008 1143 1302
Size: 22 845 141 135 159
Fragments arranged by size:
845 159 141 135 22
ApaI G_GGCC'C
Cuts at: 0 396 934 1302
Size: 396 538 368
Fragments arranged by size:
538 396 368
AvaI C'yCGr_G
Cuts at: 0 934 971 1302
Size: 934 37 331
Fragments arranged by size:
934 331 37
BanII G_rGCy'C
Cuts at: 0 234 260 396 535 721 934 1302
Size: 234 26 136 139 186 213 368
Fragments arranged by size:
368 234 213 186 139 136 26
BclI T'GATC_A
Cuts at: 0 101 858 1302
Size: 101 757 444
Fragments arranged by size:
757 444 101
BglI GCCn_nnn'nGGC
Cuts at: 0 13 631 1195 1302
Size: 13 618 564 107
Fragments arranged by size:
618 564 107 13
BlpI GC'TnA_GC
Cuts at: 0 233 904 1302
Size: 233 671 398
Fragments arranged by size:
671 398 233
Bme1580I G_kGCm'C
Cuts at: 0 396 795 934 1302
Size: 396 399 139 368
Fragments arranged by size:
399 396 368 139
BmrI ACTGGGnnnn_n'
Cuts at: 0 62 1302
Size: 62 1240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 910 1090 1302
Size: 274 636 180 212
Fragments arranged by size:
636 274 212 180
Bpu10I CC'TnA_GC
Cuts at: 0 826 1302
Size: 826 476
BsaAI yAC'GTr
Cuts at: 0 1244 1302
Size: 1244 58
BsaHI Gr'CG_yC
Cuts at: 0 515 665 1302
Size: 515 150 637
Fragments arranged by size:
637 515 150
BsaWI w'CCGG_w
Cuts at: 0 54 1302
Size: 54 1248
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 1302
Size: 358 30 914
Fragments arranged by size:
914 358 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 604 1302
Size: 325 279 698
Fragments arranged by size:
698 325 279
BseYI C'CCAG_C
Cuts at: 0 576 762 1001 1302
Size: 576 186 239 301
Fragments arranged by size:
576 301 239 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 697 874 1096 1302
Size: 493 204 177 222 206
Fragments arranged by size:
493 222 206 204 177
BsiHKAI G_wGCw'C
Cuts at: 0 758 1302
Size: 758 544
BsiWI C'GTAC_G
Cuts at: 0 708 1302
Size: 708 594
BsmI GAATG_Cn'
Cuts at: 0 1040 1154 1302
Size: 1040 114 148
Fragments arranged by size:
1040 148 114
Bsp1286I G_dGCh'C
Cuts at: 0 234 260 396 535 721 758 795 934
Size: 234 26 136 139 186 37 37 139
Cuts at: 934 1302
Size: 368
Fragments arranged by size:
368 234 186 139 139 136 37 37
26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 617 951 1302
Size: 121 496 334 351
Fragments arranged by size:
496 351 334 121
BsrBI CCG'CTC
Cuts at: 0 479 1302
Size: 479 823
BsrDI GCAATG_nn'
Cuts at: 0 995 1302
Size: 995 307
BsrFI r'CCGG_y
Cuts at: 0 54 508 1302
Size: 54 454 794
Fragments arranged by size:
794 454 54
BssSI C'ACGA_G
Cuts at: 0 1249 1302
Size: 1249 53
BstAPI GCAn_nnn'nTGC
Cuts at: 0 136 1302
Size: 136 1166
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 651 1302
Size: 75 576 651
Fragments arranged by size:
651 576 75
BtgI C'CryG_G
Cuts at: 0 338 462 1302
Size: 338 124 840
Fragments arranged by size:
840 338 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 624 1302
Size: 624 678
DraIII CAC_nnn'GTG
Cuts at: 0 1212 1302
Size: 1212 90
EaeI y'GGCC_r
Cuts at: 0 86 428 1302
Size: 86 342 874
Fragments arranged by size:
874 342 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 370 613 1302
Size: 370 243 689
Fragments arranged by size:
689 370 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 910 1090 1302
Size: 274 636 180 212
Fragments arranged by size:
636 274 212 180
EcoO109I rG'GnC_Cy
Cuts at: 0 203 348 392 822 930 931 1047 1101
Size: 203 145 44 430 108 1 116 54
Cuts at: 1101 1140 1197 1255 1302
Size: 39 57 58 47
Fragments arranged by size:
430 203 145 116 108 58 57 54
47 44 39 1
HaeII r_GCGC'y
Cuts at: 0 416 979 1190 1302
Size: 416 563 211 112
Fragments arranged by size:
563 416 211 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 358 390 391 423 655 687
Size: 109 32 217 32 1 32 232 32
Cuts at: 687 1302
Size: 615
Fragments arranged by size:
615 232 217 109 32 32 32 32
1
HincII GTy'rAC
Cuts at: 0 567 1302
Size: 567 735
HindIII A'AGCT_T
Cuts at: 0 769 1302
Size: 769 533
MfeI C'AATT_G
Cuts at: 0 436 1302
Size: 436 866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 422 566 707 1302
Size: 287 135 144 141 595
Fragments arranged by size:
595 287 144 141 135
MscI TGG'CCA
Cuts at: 0 88 430 1302
Size: 88 342 872
Fragments arranged by size:
872 342 88
MslI CAynn'nnrTG
Cuts at: 0 37 749 1060 1302
Size: 37 712 311 242
Fragments arranged by size:
712 311 242 37
MspA1I CmG'CkG
Cuts at: 0 227 464 687 813 908 1302
Size: 227 237 223 126 95 394
Fragments arranged by size:
394 237 227 223 126 95
NspI r_CATG'y
Cuts at: 0 96 1302
Size: 96 1206
PasI CC'CwG_GG
Cuts at: 0 1029 1302
Size: 1029 273
PflMI CCAn_nnn'nTGG
Cuts at: 0 402 1212 1302
Size: 402 810 90
Fragments arranged by size:
810 402 90
PfoI T'CCnGG_A
Cuts at: 0 989 1136 1302
Size: 989 147 166
Fragments arranged by size:
989 166 147
PmlI CAC'GTG
Cuts at: 0 1244 1302
Size: 1244 58
PpuMI rG'GwC_Cy
Cuts at: 0 203 348 1101 1140 1255 1302
Size: 203 145 753 39 115 47
Fragments arranged by size:
753 203 145 115 47 39
PspOMI G'GGCC_C
Cuts at: 0 392 930 1302
Size: 392 538 372
Fragments arranged by size:
538 392 372
PstI C_TGCA'G
Cuts at: 0 332 812 1013 1302
Size: 332 480 201 289
Fragments arranged by size:
480 332 289 201
PvuII CAG'CTG
Cuts at: 0 813 908 1302
Size: 813 95 394
Fragments arranged by size:
813 394 95
SacII CC_GC'GG
Cuts at: 0 465 1302
Size: 465 837
SalI G'TCGA_C
Cuts at: 0 565 1302
Size: 565 737
SanDI GG'GwC_CC
Cuts at: 0 1255 1302
Size: 1255 47
SexAI A'CCwGG_T
Cuts at: 0 1070 1302
Size: 1070 232
SfcI C'TryA_G
Cuts at: 0 328 808 1009 1302
Size: 328 480 201 293
Fragments arranged by size:
480 328 293 201
SmaI CCC'GGG
Cuts at: 0 973 1302
Size: 973 329
SphI G_CATG'C
Cuts at: 0 96 1302
Size: 96 1206
StyI C'CwwG_G
Cuts at: 0 146 362 416 1302
Size: 146 216 54 886
Fragments arranged by size:
886 216 146 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 448 1302
Size: 49 399 854
Fragments arranged by size:
854 399 49
Tth111I GACn'n_nGTC
Cuts at: 0 495 1302
Size: 495 807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 117 423 1155 1302
Size: 117 306 732 147
Fragments arranged by size:
732 306 147 117
XmaI C'CCGG_G
Cuts at: 0 971 1302
Size: 971 331
ZraI GAC'GTC
Cuts at: 0 666 1302
Size: 666 636
Enzymes that do cut:
AarI AatII AccI AgeI AhdI AleI AlwNI ApaI
AvaI BanII BclI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BtgI BtgZI DraIII EaeI EciI
Eco57MI EcoO109I HaeII Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacII SalI SanDI SexAI
SfcI SmaI SphI StyI TaqII Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BciVI BfrBI BglII
BmgBI BmtI BplI BpuEI BsaI BsaBI BsiEI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstEII BstYI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DrdI EagI EarI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KasI KpnI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TatI TstI
XbaI XhoI XmnI