Restriction map for
pF1KB7601
(Linear) (Six-base) MAPSORT of: KIBB7601.seq Check: 8271 from: 1 to: 876
KIBB7601.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 522 536 559 637 876
Size: 522 14 23 78 239
Fragments arranged by size:
522 239 78 23 14
AccI GT'mk_AC
Cuts at: 0 278 876
Size: 278 598
AgeI A'CCGG_T
Cuts at: 0 794 876
Size: 794 82
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 876
Size: 223 653
AlwNI CAG_nnn'CTG
Cuts at: 0 662 876
Size: 662 214
ApaI G_GGCC'C
Cuts at: 0 388 762 874 876
Size: 388 374 112 2
Fragments arranged by size:
388 374 112 2
AvaI C'yCGr_G
Cuts at: 0 128 284 437 444 867 876
Size: 128 156 153 7 423 9
Fragments arranged by size:
423 156 153 128 9 7
BanI G'GyrC_C
Cuts at: 0 85 114 506 586 797 876
Size: 85 29 392 80 211 79
Fragments arranged by size:
392 211 85 80 79 29
BanII G_rGCy'C
Cuts at: 0 388 405 444 762 874 876
Size: 388 17 39 318 112 2
Fragments arranged by size:
388 318 112 39 17 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 537 876
Size: 537 339
BglI GCCn_nnn'nGGC
Cuts at: 0 295 499 876
Size: 295 204 377
Fragments arranged by size:
377 295 204
BglII A'GATC_T
Cuts at: 0 635 876
Size: 635 241
BlpI GC'TnA_GC
Cuts at: 0 425 876
Size: 425 451
Bme1580I G_kGCm'C
Cuts at: 0 119 155 388 591 762 874 876
Size: 119 36 233 203 171 112 2
Fragments arranged by size:
233 203 171 119 112 36 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 82 639 876
Size: 15 67 557 237
Fragments arranged by size:
557 237 67 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 876
Size: 577 299
BsaBI GATnn'nnATC
Cuts at: 0 601 876
Size: 601 275
BsaHI Gr'CG_yC
Cuts at: 0 257 782 876
Size: 257 525 94
Fragments arranged by size:
525 257 94
BsaWI w'CCGG_w
Cuts at: 0 405 794 876
Size: 405 389 82
Fragments arranged by size:
405 389 82
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 174 865 876
Size: 112 62 691 11
Fragments arranged by size:
691 112 62 11
BseYI C'CCAG_C
Cuts at: 0 167 876
Size: 167 709
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 609 792 876
Size: 502 107 183 84
Fragments arranged by size:
502 183 107 84
BsiEI CG_ry'CG
Cuts at: 0 191 744 876
Size: 191 553 132
Fragments arranged by size:
553 191 132
BsmBI CGTCTCn'nnnn_
Cuts at: 0 58 876
Size: 58 818
Bsp1286I G_dGCh'C
Cuts at: 0 119 155 388 405 444 591 762 874
Size: 119 36 233 17 39 147 171 112
Cuts at: 874 876
Size: 2
Fragments arranged by size:
233 171 147 119 112 39 36 17
2
BspEI T'CCGG_A
Cuts at: 0 405 876
Size: 405 471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 522 536 559 637 876
Size: 347 175 14 23 78 239
Fragments arranged by size:
347 239 175 78 23 14
BsrBI CCG'CTC
Cuts at: 0 182 429 746 876
Size: 182 247 317 130
Fragments arranged by size:
317 247 182 130
BsrFI r'CCGG_y
Cuts at: 0 53 392 509 794 800 876
Size: 53 339 117 285 6 76
Fragments arranged by size:
339 285 117 76 53 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 560 876
Size: 560 316
BstEII G'GTnAC_C
Cuts at: 0 157 876
Size: 157 719
BstYI r'GATC_y
Cuts at: 0 635 876
Size: 635 241
BtgI C'CryG_G
Cuts at: 0 220 876
Size: 220 656
BtsI GCAGTG_nn'
Cuts at: 0 346 465 519 720 876
Size: 346 119 54 201 156
Fragments arranged by size:
346 201 156 119 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 621 656 876
Size: 621 35 220
Fragments arranged by size:
621 220 35
DraIII CAC_nnn'GTG
Cuts at: 0 368 876
Size: 368 508
EaeI y'GGCC_r
Cuts at: 0 188 491 500 876
Size: 188 303 9 376
Fragments arranged by size:
376 303 188 9
EagI C'GGCC_G
Cuts at: 0 188 876
Size: 188 688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 82 639 876
Size: 15 67 557 237
Fragments arranged by size:
557 237 67 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 654 876
Size: 654 222
EcoO109I rG'GnC_Cy
Cuts at: 0 42 141 242 659 731 871 876
Size: 42 99 101 417 72 140 5
Fragments arranged by size:
417 140 101 99 72 42 5
HaeII r_GCGC'y
Cuts at: 0 807 876
Size: 807 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 876
Size: 169 32 675
Fragments arranged by size:
675 169 32
HincII GTy'rAC
Cuts at: 0 279 876
Size: 279 597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 876
Size: 773 103
MscI TGG'CCA
Cuts at: 0 502 876
Size: 502 374
MspA1I CmG'CkG
Cuts at: 0 516 876
Size: 516 360
NaeI GCC'GGC
Cuts at: 0 802 876
Size: 802 74
NgoMIV G'CCGG_C
Cuts at: 0 800 876
Size: 800 76
NspI r_CATG'y
Cuts at: 0 831 876
Size: 831 45
PasI CC'CwG_GG
Cuts at: 0 173 876
Size: 173 703
PflMI CCAn_nnn'nTGG
Cuts at: 0 368 489 662 876
Size: 368 121 173 214
Fragments arranged by size:
368 214 173 121
PpuMI rG'GwC_Cy
Cuts at: 0 42 876
Size: 42 834
PspOMI G'GGCC_C
Cuts at: 0 384 758 870 876
Size: 384 374 112 6
Fragments arranged by size:
384 374 112 6
PstI C_TGCA'G
Cuts at: 0 533 674 727 876
Size: 533 141 53 149
Fragments arranged by size:
533 149 141 53
PvuII CAG'CTG
Cuts at: 0 516 876
Size: 516 360
SalI G'TCGA_C
Cuts at: 0 277 876
Size: 277 599
SanDI GG'GwC_CC
Cuts at: 0 42 876
Size: 42 834
SbfI CC_TGCA'GG
Cuts at: 0 533 876
Size: 533 343
ScaI AGT'ACT
Cuts at: 0 559 876
Size: 559 317
SfcI C'TryA_G
Cuts at: 0 529 670 723 876
Size: 529 141 53 153
Fragments arranged by size:
529 153 141 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 295 499 876
Size: 295 204 377
Fragments arranged by size:
377 295 204
SmaI CCC'GGG
Cuts at: 0 446 869 876
Size: 446 423 7
Fragments arranged by size:
446 423 7
SmlI C'TyrA_G
Cuts at: 0 592 876
Size: 592 284
SrfI GCCC'GGGC
Cuts at: 0 869 876
Size: 869 7
StuI AGG'CCT
Cuts at: 0 649 876
Size: 649 227
StyI C'CwwG_G
Cuts at: 0 260 380 876
Size: 260 120 496
Fragments arranged by size:
496 260 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 574 703 876
Size: 574 129 173
Fragments arranged by size:
574 173 129
TatI w'GTAC_w
Cuts at: 0 557 572 876
Size: 557 15 304
Fragments arranged by size:
557 304 15
XmaI C'CCGG_G
Cuts at: 0 444 867 876
Size: 444 423 9
Fragments arranged by size:
444 423 9
Enzymes that do cut:
AarI AccI AgeI AlfI AlwNI ApaI AvaI BanI
BanII BbsI BglI BglII BlpI Bme1580I BpmI BpuEI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstEII BstYI
BtgI BtsI CspCI DraIII EaeI EagI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MmeI MscI MspA1I NaeI
NgoMIV NspI PasI PflMI PpuMI PspOMI PstI PvuII
SalI SanDI SbfI ScaI SfcI SfiI SmaI SmlI
SrfI StuI StyI TaqII TatI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaXI
BsiHKAI BsiWI BsmI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MslI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SapI SexAI SfoI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI