Restriction map for
pF1KB7614
(Linear) (Six-base) MAPSORT of: KIBB7614.seq Check: 3567 from: 1 to: 1080
KIBB7614.orf
With 182 enzymes: SgfI *
June 8, 2009 13:41 ..
Acc65I G'GTAC_C
Cuts at: 0 961 1080
Size: 961 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 634 1080
Size: 478 156 446
Fragments arranged by size:
478 446 156
AflIII A'CryG_T
Cuts at: 0 587 1080
Size: 587 493
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 727 759 1080
Size: 727 32 321
Fragments arranged by size:
727 321 32
AlwNI CAG_nnn'CTG
Cuts at: 0 163 1080
Size: 163 917
ApaI G_GGCC'C
Cuts at: 0 233 953 1080
Size: 233 720 127
Fragments arranged by size:
720 233 127
ApoI r'AATT_y
Cuts at: 0 738 1080
Size: 738 342
AvaI C'yCGr_G
Cuts at: 0 436 805 1080
Size: 436 369 275
Fragments arranged by size:
436 369 275
BanI G'GyrC_C
Cuts at: 0 358 842 961 1080
Size: 358 484 119 119
Fragments arranged by size:
484 358 119 119
BanII G_rGCy'C
Cuts at: 0 233 341 506 806 953 982 1063 1080
Size: 233 108 165 300 147 29 81 17
Fragments arranged by size:
300 233 165 147 108 81 29 17
BbeI G_GCGC'C
Cuts at: 0 846 1080
Size: 846 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 461 1080
Size: 427 34 619
Fragments arranged by size:
619 427 34
BfrBI ATG'CAT
Cuts at: 0 1071 1080
Size: 1071 9
BglI GCCn_nnn'nGGC
Cuts at: 0 69 851 1080
Size: 69 782 229
Fragments arranged by size:
782 229 69
BlpI GC'TnA_GC
Cuts at: 0 106 574 1080
Size: 106 468 506
Fragments arranged by size:
506 468 106
Bme1580I G_kGCm'C
Cuts at: 0 233 257 757 953 1080
Size: 233 24 500 196 127
Fragments arranged by size:
500 233 196 127 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 333 1080
Size: 333 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 146 391 406 754 1080
Size: 26 120 245 15 348 326
Fragments arranged by size:
348 326 245 120 26 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 265 911 1080
Size: 265 646 169
Fragments arranged by size:
646 265 169
BsaHI Gr'CG_yC
Cuts at: 0 843 1080
Size: 843 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 290 320 727 757 1080
Size: 290 30 407 30 323
Fragments arranged by size:
407 323 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 993 1080
Size: 415 578 87
Fragments arranged by size:
578 415 87
BseYI C'CCAG_C
Cuts at: 0 83 791 901 1080
Size: 83 708 110 179
Fragments arranged by size:
708 179 110 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 700 977 1080
Size: 327 373 277 103
Fragments arranged by size:
373 327 277 103
BsmI GAATG_Cn'
Cuts at: 0 1071 1080
Size: 1071 9
Bsp1286I G_dGCh'C
Cuts at: 0 233 257 341 506 757 806 953 982
Size: 233 24 84 165 251 49 147 29
Cuts at: 982 1063 1080
Size: 81 17
Fragments arranged by size:
251 233 165 147 84 81 49 29
24 17
BsrFI r'CCGG_y
Cuts at: 0 839 1080
Size: 839 241
BssSI C'ACGA_G
Cuts at: 0 676 1080
Size: 676 404
BstXI CCAn_nnnn'nTGG
Cuts at: 0 799 1080
Size: 799 281
Bsu36I CC'TnA_GG
Cuts at: 0 284 1019 1080
Size: 284 735 61
Fragments arranged by size:
735 284 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 771 1080
Size: 771 309
BtsI GCAGTG_nn'
Cuts at: 0 757 766 989 1080
Size: 757 9 223 91
Fragments arranged by size:
757 223 91 9
EarI CTCTTCn'nnn_
Cuts at: 0 460 1080
Size: 460 620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 146 391 406 478 634 754 1080
Size: 26 120 245 15 72 156 120 326
Fragments arranged by size:
326 245 156 120 120 72 26 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 125 679 914 1080
Size: 125 554 235 166
Fragments arranged by size:
554 235 166 125
EcoO109I rG'GnC_Cy
Cuts at: 0 118 230 326 949 950 1080
Size: 118 112 96 623 1 130
Fragments arranged by size:
623 130 118 112 96 1
HaeII r_GCGC'y
Cuts at: 0 846 1080
Size: 846 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 1080
Size: 313 32 735
Fragments arranged by size:
735 313 32
HindIII A'AGCT_T
Cuts at: 0 108 1080
Size: 108 972
KasI G'GCGC_C
Cuts at: 0 842 1080
Size: 842 238
KpnI G_GTAC'C
Cuts at: 0 965 1080
Size: 965 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 1080
Size: 219 861
NarI GG'CG_CC
Cuts at: 0 843 1080
Size: 843 237
NsiI A_TGCA'T
Cuts at: 0 1073 1080
Size: 1073 7
NspI r_CATG'y
Cuts at: 0 591 1080
Size: 591 489
PasI CC'CwG_GG
Cuts at: 0 477 510 946 1080
Size: 477 33 436 134
Fragments arranged by size:
477 436 134 33
PciI A'CATG_T
Cuts at: 0 587 1080
Size: 587 493
PflMI CCAn_nnn'nTGG
Cuts at: 0 49 973 1080
Size: 49 924 107
Fragments arranged by size:
924 107 49
PfoI T'CCnGG_A
Cuts at: 0 123 1080
Size: 123 957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 1080
Size: 727 32 321
Fragments arranged by size:
727 321 32
PshAI GACnn'nnGTC
Cuts at: 0 927 1080
Size: 927 153
PspOMI G'GGCC_C
Cuts at: 0 229 949 1080
Size: 229 720 131
Fragments arranged by size:
720 229 131
PspXI vC'TCGA_Gb
Cuts at: 0 436 1080
Size: 436 644
PstI C_TGCA'G
Cuts at: 0 428 572 614 752 773 1080
Size: 428 144 42 138 21 307
Fragments arranged by size:
428 307 144 138 42 21
SfcI C'TryA_G
Cuts at: 0 424 568 610 748 769 1080
Size: 424 144 42 138 21 311
Fragments arranged by size:
424 311 144 138 42 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 69 1080
Size: 69 1011
SfoI GGC'GCC
Cuts at: 0 844 1080
Size: 844 236
SmlI C'TyrA_G
Cuts at: 0 436 805 1080
Size: 436 369 275
Fragments arranged by size:
436 369 275
StyI C'CwwG_G
Cuts at: 0 114 258 1080
Size: 114 144 822
Fragments arranged by size:
822 144 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 609 996 1080
Size: 609 387 84
Fragments arranged by size:
609 387 84
TatI w'GTAC_w
Cuts at: 0 998 1080
Size: 998 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 290 322 1080
Size: 290 32 758
Fragments arranged by size:
758 290 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 121 1080
Size: 121 959
XhoI C'TCGA_G
Cuts at: 0 436 805 1080
Size: 436 369 275
Fragments arranged by size:
436 369 275
XmnI GAAnn'nnTTC
Cuts at: 0 532 647 742 1031 1080
Size: 532 115 95 289 49
Fragments arranged by size:
532 289 115 95 49
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BcgI BfrBI BglI BlpI Bme1580I
BplI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsmI Bsp1286I BsrFI BssSI BstXI Bsu36I BtgZI BtsI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI
KpnI MmeI NarI NsiI NspI PasI PciI PflMI
PfoI PpiI PshAI PspOMI PspXI PstI SfcI SfiI
SfoI SmlI StyI TaqII TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BglII BmgBI BmrI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I BtgI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
MfeI MluI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI PmeI PmlI PpuMI
PsiI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XmaI ZraI