(Linear) (Six-base) MAPSORT of: KIBB7617.seq  Check: 7297  from: 1  to: 921



KIBB7617.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AflII C'TTAA_G

Cuts at:      0      34     921 
   Size:         34     887
 
AhdI GACnn_n'nnGTC

Cuts at:      0     594     921 
   Size:        594     327
 
AleI CACnn'nnGTG

Cuts at:      0     619     921 
   Size:        619     302
 
ApoI r'AATT_y

Cuts at:      0      29      55     169     208     426     488     921 
   Size:         29      26     114      39     218      62     433

  Fragments arranged by size: 

                 433     218     114      62      39      29      26
 
BanII G_rGCy'C

Cuts at:      0     280     921 
   Size:        280     641
 
BlpI GC'TnA_GC

Cuts at:      0     535     921 
   Size:        535     386
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     921 
   Size:        418     503
 
BsaBI GATnn'nnATC

Cuts at:      0     392     921 
   Size:        392     529
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     690     720     921 
   Size:        690      30     201

  Fragments arranged by size: 

                 690     201      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     626     921 
   Size:        626     295
 
BsiHKAI G_wGCw'C

Cuts at:      0     280     921 
   Size:        280     641
 
BsmI GAATG_Cn'

Cuts at:      0      42     791     921 
   Size:         42     749     130

  Fragments arranged by size: 

                 749     130      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     280     921 
   Size:        280     641
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     694     921 
   Size:        694     227
 
BsrDI GCAATG_nn'

Cuts at:      0     499     921 
   Size:        499     422
 
BsrGI T'GTAC_A

Cuts at:      0     375     921 
   Size:        375     546
 
BstYI r'GATC_y

Cuts at:      0     128     921 
   Size:        128     793
 
EaeI y'GGCC_r

Cuts at:      0     407     921 
   Size:        407     514
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     578     921 
   Size:        578     343
 
EcoICRI GAG'CTC

Cuts at:      0     278     921 
   Size:        278     643
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     921 
   Size:        418     503
 
EcoRI G'AATT_C

Cuts at:      0     169     921 
   Size:        169     752
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     354     386     580     612     921 
   Size:        354      32     194      32     309

  Fragments arranged by size: 

                 354     309     194      32      32
 
HincII GTy'rAC

Cuts at:      0     895     921 
   Size:        895      26
 
HindIII A'AGCT_T

Cuts at:      0     136     921 
   Size:        136     785
 
MslI CAynn'nnrTG

Cuts at:      0     619     796     921 
   Size:        619     177     125

  Fragments arranged by size: 

                 619     177     125
 
PacI TTA_AT'TAA

Cuts at:      0     827     921 
   Size:        827      94
 
PfoI T'CCnGG_A

Cuts at:      0     598     921 
   Size:        598     323
 
PsiI TTA'TAA

Cuts at:      0      61     921 
   Size:         61     860
 
SacI G_AGCT'C

Cuts at:      0     280     921 
   Size:        280     641
 
SmlI C'TyrA_G

Cuts at:      0      34     921 
   Size:         34     887
 
SspI AAT'ATT

Cuts at:      0     111     921 
   Size:        111     810
 
TatI w'GTAC_w

Cuts at:      0     375     921 
   Size:        375     546
 
XmnI GAAnn'nnTTC

Cuts at:      0     791     921 
   Size:        791     130
 

 Enzymes that do cut:

    AflII     AhdI     AleI     ApoI    BanII     BlpI     BpmI    BsaBI
    BsaXI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrGI
    BstYI     EaeI     EciI  EcoICRI  Eco57MI    EcoRI    Hin4I   HincII
  HindIII     MslI     PacI     PfoI     PsiI     SacI     SmlI     SspI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI