Restriction map for
pF1KB7619
(Linear) (Six-base) MAPSORT of: KIBB7619.seq Check: 728 from: 1 to: 924
KIBB7619.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 924
Size: 664 260
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 76 108 924
Size: 76 32 816
Fragments arranged by size:
816 76 32
ApoI r'AATT_y
Cuts at: 0 135 268 869 924
Size: 135 133 601 55
Fragments arranged by size:
601 135 133 55
BamHI G'GATC_C
Cuts at: 0 403 924
Size: 403 521
BanI G'GyrC_C
Cuts at: 0 648 924
Size: 648 276
BanII G_rGCy'C
Cuts at: 0 605 924
Size: 605 319
BbsI GAAGACnn'nnnn_
Cuts at: 0 509 924
Size: 509 415
BbvCI CC'TCA_GC
Cuts at: 0 315 924
Size: 315 609
BfrBI ATG'CAT
Cuts at: 0 110 924
Size: 110 814
BglII A'GATC_T
Cuts at: 0 348 712 924
Size: 348 364 212
Fragments arranged by size:
364 348 212
BmrI ACTGGGnnnn_n'
Cuts at: 0 730 924
Size: 730 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 924
Size: 866 58
Bpu10I CC'TnA_GC
Cuts at: 0 315 924
Size: 315 609
BsaI GGTCTCn'nnnn_
Cuts at: 0 242 924
Size: 242 682
BsaAI yAC'GTr
Cuts at: 0 851 924
Size: 851 73
BsaBI GATnn'nnATC
Cuts at: 0 704 924
Size: 704 220
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 924
Size: 595 329
BseYI C'CCAG_C
Cuts at: 0 129 924
Size: 129 795
Bsp1286I G_dGCh'C
Cuts at: 0 605 924
Size: 605 319
BspHI T'CATG_A
Cuts at: 0 149 530 924
Size: 149 381 394
Fragments arranged by size:
394 381 149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 678 924
Size: 678 246
BstXI CCAn_nnnn'nTGG
Cuts at: 0 62 924
Size: 62 862
BstYI r'GATC_y
Cuts at: 0 348 403 712 924
Size: 348 55 309 212
Fragments arranged by size:
348 309 212 55
DraI TTT'AAA
Cuts at: 0 867 924
Size: 867 57
EaeI y'GGCC_r
Cuts at: 0 410 924
Size: 410 514
EarI CTCTTCn'nnn_
Cuts at: 0 332 671 924
Size: 332 339 253
Fragments arranged by size:
339 332 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 866 924
Size: 664 202 58
Fragments arranged by size:
664 202 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 610 924
Size: 610 314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 168 200 333 365 580 612 648 680
Size: 168 32 133 32 215 32 36 32
Cuts at: 680 697 729 924
Size: 17 32 195
Fragments arranged by size:
215 195 168 133 36 32 32 32
32 32 17
HincII GTy'rAC
Cuts at: 0 379 747 924
Size: 379 368 177
Fragments arranged by size:
379 368 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 617 633 924
Size: 185 432 16 291
Fragments arranged by size:
432 291 185 16
MscI TGG'CCA
Cuts at: 0 412 924
Size: 412 512
NsiI A_TGCA'T
Cuts at: 0 112 924
Size: 112 812
PfoI T'CCnGG_A
Cuts at: 0 213 924
Size: 213 711
PstI C_TGCA'G
Cuts at: 0 501 674 924
Size: 501 173 250
Fragments arranged by size:
501 250 173
SfcI C'TryA_G
Cuts at: 0 497 670 750 924
Size: 497 173 80 174
Fragments arranged by size:
497 174 173 80
SspI AAT'ATT
Cuts at: 0 564 821 924
Size: 564 257 103
Fragments arranged by size:
564 257 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 675 924
Size: 675 249
TatI w'GTAC_w
Cuts at: 0 844 924
Size: 844 80
Enzymes that do cut:
AcuI AloI ApoI BamHI BanI BanII BbsI BbvCI
BfrBI BglII BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BseRI BseYI Bsp1286I BspHI BstAPI BstXI BstYI DraI
EaeI EarI Eco57MI EcoNI Hin4I HincII MmeI MscI
NsiI PfoI PstI SfcI SspI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI
BclI BglI BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI