(Linear) (Six-base) MAPSORT of: KIBB7624.seq  Check: 8328  from: 1  to: 1104



KIBB7624.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     485    1104 
   Size:        322     163     619

  Fragments arranged by size: 

                 619     322     163
 
AflIII A'CryG_T

Cuts at:      0     155     573    1104 
   Size:        155     418     531

  Fragments arranged by size: 

                 531     418     155
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     377    1104 
   Size:        377     727
 
ApoI r'AATT_y

Cuts at:      0     141     325     452     478     744    1104 
   Size:        141     184     127      26     266     360

  Fragments arranged by size: 

                 360     266     184     141     127      26
 
BanII G_rGCy'C

Cuts at:      0      95    1104 
   Size:         95    1009
 
BbvCI CC'TCA_GC

Cuts at:      0     613    1104 
   Size:        613     491
 
BclI T'GATC_A

Cuts at:      0     552    1104 
   Size:        552     552
 
BlpI GC'TnA_GC

Cuts at:      0      43    1104 
   Size:         43    1061
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117    1104 
   Size:        117     987
 
Bpu10I CC'TnA_GC

Cuts at:      0     613    1104 
   Size:        613     491
 
BsaBI GATnn'nnATC

Cuts at:      0     557    1104 
   Size:        557     547
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1003    1104 
   Size:       1003     101
 
BsiHKAI G_wGCw'C

Cuts at:      0      95    1104 
   Size:         95    1009
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      52    1104 
   Size:         52    1052
 
Bsp1286I G_dGCh'C

Cuts at:      0      95    1104 
   Size:         95    1009
 
BspHI T'CATG_A

Cuts at:      0     549    1104 
   Size:        549     555
 
BsrDI GCAATG_nn'

Cuts at:      0     422     984    1104 
   Size:        422     562     120

  Fragments arranged by size: 

                 562     422     120
 
BtsI GCAGTG_nn'

Cuts at:      0     679    1076    1104 
   Size:        679     397      28

  Fragments arranged by size: 

                 679     397      28
 
DraI TTT'AAA

Cuts at:      0     323    1104 
   Size:        323     781
 
EarI CTCTTCn'nnn_

Cuts at:      0     870    1040    1084    1104 
   Size:        870     170      44      20

  Fragments arranged by size: 

                 870     170      44      20
 
EcoICRI GAG'CTC

Cuts at:      0      93    1104 
   Size:         93    1011
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     322     485    1104 
   Size:        117     205     163     619

  Fragments arranged by size: 

                 619     205     163     117
 
EcoO109I rG'GnC_Cy

Cuts at:      0     486    1104 
   Size:        486     618
 
HaeII r_GCGC'y

Cuts at:      0      40    1104 
   Size:         40    1064
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     868     900    1104 
   Size:        868      32     204

  Fragments arranged by size: 

                 868     204      32
 
HincII GTy'rAC

Cuts at:      0     274    1104 
   Size:        274     830
 
HindIII A'AGCT_T

Cuts at:      0     170    1104 
   Size:        170     934
 
MslI CAynn'nnrTG

Cuts at:      0     359    1104 
   Size:        359     745
 
NspI r_CATG'y

Cuts at:      0     159     577    1104 
   Size:        159     418     527

  Fragments arranged by size: 

                 527     418     159
 
PciI A'CATG_T

Cuts at:      0     155     573    1104 
   Size:        155     418     531

  Fragments arranged by size: 

                 531     418     155
 
PfoI T'CCnGG_A

Cuts at:      0      53    1104 
   Size:         53    1051
 
PpuMI rG'GwC_Cy

Cuts at:      0     486    1104 
   Size:        486     618
 
PstI C_TGCA'G

Cuts at:      0      23    1104 
   Size:         23    1081
 
SacI G_AGCT'C

Cuts at:      0      95    1104 
   Size:         95    1009
 
SfcI C'TryA_G

Cuts at:      0      19    1104 
   Size:         19    1085
 
SspI AAT'ATT

Cuts at:      0     645    1104 
   Size:        645     459
 
StuI AGG'CCT

Cuts at:      0     805    1104 
   Size:        805     299
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      40    1104 
   Size:         40    1064
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     223    1104 
   Size:        223     881
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI    BanII    BbvCI     BclI     BlpI
     BpmI   Bpu10I    BsaBI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BsrDI     BtsI     DraI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII  HindIII     MslI     NspI     PciI     PfoI    PpuMI
     PstI     SacI     SfcI     SspI     StuI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI