Restriction map for
pF1KB7627
(Linear) (Six-base) MAPSORT of: KIBB7627.seq Check: 218 from: 1 to: 891
KIBB7627.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 58 891
Size: 58 833
AhdI GACnn_n'nnGTC
Cuts at: 0 43 891
Size: 43 848
ApaLI G'TGCA_C
Cuts at: 0 318 891
Size: 318 573
AvaI C'yCGr_G
Cuts at: 0 652 891
Size: 652 239
BanII G_rGCy'C
Cuts at: 0 470 863 891
Size: 470 393 28
Fragments arranged by size:
470 393 28
Bme1580I G_kGCm'C
Cuts at: 0 322 398 872 891
Size: 322 76 474 19
Fragments arranged by size:
474 322 76 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 747 891
Size: 33 714 144
Fragments arranged by size:
714 144 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 747 891
Size: 747 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 866 877 891
Size: 827 39 11 14
Fragments arranged by size:
827 39 14 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 583 808 891
Size: 583 225 83
Fragments arranged by size:
583 225 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 891
Size: 125 766
BseYI C'CCAG_C
Cuts at: 0 155 580 632 883 891
Size: 155 425 52 251 8
Fragments arranged by size:
425 251 155 52 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 207 699 891
Size: 105 102 492 192
Fragments arranged by size:
492 192 105 102
BsiHKAI G_wGCw'C
Cuts at: 0 322 863 891
Size: 322 541 28
Fragments arranged by size:
541 322 28
BsmI GAATG_Cn'
Cuts at: 0 763 891
Size: 763 128
Bsp1286I G_dGCh'C
Cuts at: 0 322 398 470 863 872 891
Size: 322 76 72 393 9 19
Fragments arranged by size:
393 322 76 72 19 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 58 131 782 891
Size: 58 73 651 109
Fragments arranged by size:
651 109 73 58
BsrDI GCAATG_nn'
Cuts at: 0 504 891
Size: 504 387
BstAPI GCAn_nnn'nTGC
Cuts at: 0 128 317 891
Size: 128 189 574
Fragments arranged by size:
574 189 128
BstXI CCAn_nnnn'nTGG
Cuts at: 0 61 891
Size: 61 830
Bsu36I CC'TnA_GG
Cuts at: 0 401 891
Size: 401 490
BtgI C'CryG_G
Cuts at: 0 704 891
Size: 704 187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 72 107 399 434 891
Size: 72 35 292 35 457
Fragments arranged by size:
457 292 72 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 475 571 891
Size: 475 96 320
Fragments arranged by size:
475 320 96
EcoICRI GAG'CTC
Cuts at: 0 861 891
Size: 861 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 866 877 891
Size: 827 39 11 14
Fragments arranged by size:
827 39 14 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 510 891
Size: 510 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 652 684 891
Size: 29 32 591 32 207
Fragments arranged by size:
591 207 32 32 29
HincII GTy'rAC
Cuts at: 0 219 891
Size: 219 672
MspA1I CmG'CkG
Cuts at: 0 155 891
Size: 155 736
NcoI C'CATG_G
Cuts at: 0 704 891
Size: 704 187
PfoI T'CCnGG_A
Cuts at: 0 66 891
Size: 66 825
PspXI vC'TCGA_Gb
Cuts at: 0 652 891
Size: 652 239
PstI C_TGCA'G
Cuts at: 0 299 530 667 891
Size: 299 231 137 224
Fragments arranged by size:
299 231 224 137
PvuII CAG'CTG
Cuts at: 0 155 891
Size: 155 736
SacI G_AGCT'C
Cuts at: 0 863 891
Size: 863 28
SapI GCTCTTCn'nnn_
Cuts at: 0 475 891
Size: 475 416
SbfI CC_TGCA'GG
Cuts at: 0 299 891
Size: 299 592
ScaI AGT'ACT
Cuts at: 0 22 891
Size: 22 869
SfcI C'TryA_G
Cuts at: 0 295 526 663 891
Size: 295 231 137 228
Fragments arranged by size:
295 231 228 137
SmlI C'TyrA_G
Cuts at: 0 652 891
Size: 652 239
SpeI A'CTAG_T
Cuts at: 0 673 891
Size: 673 218
StuI AGG'CCT
Cuts at: 0 293 891
Size: 293 598
StyI C'CwwG_G
Cuts at: 0 83 704 818 891
Size: 83 621 114 73
Fragments arranged by size:
621 114 83 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 402 594 891
Size: 402 192 297
Fragments arranged by size:
402 297 192
TatI w'GTAC_w
Cuts at: 0 20 891
Size: 20 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 891
Size: 292 32 567
Fragments arranged by size:
567 292 32
XhoI C'TCGA_G
Cuts at: 0 652 891
Size: 652 239
Enzymes that do cut:
AarI AhdI ApaLI AvaI BanII Bme1580I BmrI BplI
BpmI BsaI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstAPI BstXI Bsu36I BtgI CspCI EarI
EcoICRI Eco57MI FalI Hin4I HincII MspA1I NcoI PfoI
PspXI PstI PvuII SacI SapI SbfI ScaI SfcI
SmlI SpeI StuI StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XmaI XmnI ZraI