Restriction map for
pF1KB7631
(Linear) (Six-base) MAPSORT of: KIBB7631.seq Check: 3557 from: 1 to: 960
KIBB7631.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 32 960
Size: 32 928
AfeI AGC'GCT
Cuts at: 0 400 960
Size: 400 560
AflIII A'CryG_T
Cuts at: 0 593 960
Size: 593 367
AhdI GACnn_n'nnGTC
Cuts at: 0 753 960
Size: 753 207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 869 938 960
Size: 869 69 22
Fragments arranged by size:
869 69 22
AlwNI CAG_nnn'CTG
Cuts at: 0 486 960
Size: 486 474
ApaI G_GGCC'C
Cuts at: 0 547 960
Size: 547 413
ApaLI G'TGCA_C
Cuts at: 0 205 960
Size: 205 755
AvaI C'yCGr_G
Cuts at: 0 216 555 648 718 960
Size: 216 339 93 70 242
Fragments arranged by size:
339 242 216 93 70
BanI G'GyrC_C
Cuts at: 0 257 356 517 655 682 699 795 960
Size: 257 99 161 138 27 17 96 165
Fragments arranged by size:
257 165 161 138 99 96 27 17
BanII G_rGCy'C
Cuts at: 0 547 790 960
Size: 547 243 170
Fragments arranged by size:
547 243 170
BbeI G_GCGC'C
Cuts at: 0 360 521 659 686 703 799 960
Size: 360 161 138 27 17 96 161
Fragments arranged by size:
360 161 161 138 96 27 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 137 960
Size: 137 823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 772 806 960
Size: 772 34 154
Fragments arranged by size:
772 154 34
BglI GCCn_nnn'nGGC
Cuts at: 0 255 295 566 960
Size: 255 40 271 394
Fragments arranged by size:
394 271 255 40
Bme1580I G_kGCm'C
Cuts at: 0 209 260 547 922 960
Size: 209 51 287 375 38
Fragments arranged by size:
375 287 209 51 38
BmgBI CAC'GTC
Cuts at: 0 407 819 960
Size: 407 412 141
Fragments arranged by size:
412 407 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 888 960
Size: 888 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 792 960
Size: 792 168
Bpu10I CC'TnA_GC
Cuts at: 0 364 960
Size: 364 596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 960
Size: 379 581
BsaAI yAC'GTr
Cuts at: 0 156 226 960
Size: 156 70 734
Fragments arranged by size:
734 156 70
BsaHI Gr'CG_yC
Cuts at: 0 357 503 518 656 683 700 796 905
Size: 357 146 15 138 27 17 96 109
Cuts at: 905 960
Size: 55
Fragments arranged by size:
357 146 138 109 96 55 27 17
15
BsaWI w'CCGG_w
Cuts at: 0 900 960
Size: 900 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 960
Size: 25 935
BseYI C'CCAG_C
Cuts at: 0 250 703 862 960
Size: 250 453 159 98
Fragments arranged by size:
453 250 159 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 960
Size: 387 573
BsiEI CG_ry'CG
Cuts at: 0 327 960
Size: 327 633
BsiHKAI G_wGCw'C
Cuts at: 0 185 209 575 959 960
Size: 185 24 366 384 1
Fragments arranged by size:
384 366 185 24 1
Bsp1286I G_dGCh'C
Cuts at: 0 185 209 260 547 575 790 922 959
Size: 185 24 51 287 28 215 132 37
Cuts at: 959 960
Size: 1
Fragments arranged by size:
287 215 185 132 51 37 28 24
1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 960
Size: 125 835
BsrBI CCG'CTC
Cuts at: 0 21 354 653 960
Size: 21 333 299 307
Fragments arranged by size:
333 307 299 21
BssHII G'CGCG_C
Cuts at: 0 161 243 245 960
Size: 161 82 2 715
Fragments arranged by size:
715 161 82 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 299 486 960
Size: 299 187 474
Fragments arranged by size:
474 299 187
BstBI TT'CG_AA
Cuts at: 0 128 960
Size: 128 832
BstXI CCAn_nnnn'nTGG
Cuts at: 0 765 960
Size: 765 195
BstYI r'GATC_y
Cuts at: 0 443 960
Size: 443 517
BtgI C'CryG_G
Cuts at: 0 197 561 960
Size: 197 364 399
Fragments arranged by size:
399 364 197
BtsI GCAGTG_nn'
Cuts at: 0 922 960
Size: 922 38
EarI CTCTTCn'nnn_
Cuts at: 0 173 718 960
Size: 173 545 242
Fragments arranged by size:
545 242 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 681 829 960
Size: 681 148 131
Fragments arranged by size:
681 148 131
EcoO109I rG'GnC_Cy
Cuts at: 0 474 960
Size: 474 486
FspI TGC'GCA
Cuts at: 0 451 960
Size: 451 509
HaeII r_GCGC'y
Cuts at: 0 360 402 521 659 686 703 799 960
Size: 360 42 119 138 27 17 96 161
Fragments arranged by size:
360 161 138 119 96 42 27 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 739 771 960
Size: 27 712 32 189
Fragments arranged by size:
712 189 32 27
KasI G'GCGC_C
Cuts at: 0 356 517 655 682 699 795 960
Size: 356 161 138 27 17 96 165
Fragments arranged by size:
356 165 161 138 96 27 17
MluI A'CGCG_T
Cuts at: 0 593 960
Size: 593 367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 635 644 960
Size: 614 21 9 316
Fragments arranged by size:
614 316 21 9
MspA1I CmG'CkG
Cuts at: 0 199 254 275 426 549 563 581 641
Size: 199 55 21 151 123 14 18 60
Cuts at: 641 881 960
Size: 240 79
Fragments arranged by size:
240 199 151 123 79 60 55 21
18 14
NarI GG'CG_CC
Cuts at: 0 357 518 656 683 700 796 960
Size: 357 161 138 27 17 96 164
Fragments arranged by size:
357 164 161 138 96 27 17
NspI r_CATG'y
Cuts at: 0 241 960
Size: 241 719
PmlI CAC'GTG
Cuts at: 0 156 226 960
Size: 156 70 734
Fragments arranged by size:
734 156 70
PspOMI G'GGCC_C
Cuts at: 0 543 960
Size: 543 417
PstI C_TGCA'G
Cuts at: 0 482 960
Size: 482 478
SacII CC_GC'GG
Cuts at: 0 200 564 960
Size: 200 364 396
Fragments arranged by size:
396 364 200
SapI GCTCTTCn'nnn_
Cuts at: 0 173 960
Size: 173 787
SbfI CC_TGCA'GG
Cuts at: 0 482 960
Size: 482 478
SexAI A'CCwGG_T
Cuts at: 0 950 960
Size: 950 10
SfcI C'TryA_G
Cuts at: 0 478 576 636 960
Size: 478 98 60 324
Fragments arranged by size:
478 324 98 60
SfoI GGC'GCC
Cuts at: 0 358 519 657 684 701 797 960
Size: 358 161 138 27 17 96 163
Fragments arranged by size:
358 163 161 138 96 27 17
SmaI CCC'GGG
Cuts at: 0 218 557 720 960
Size: 218 339 163 240
Fragments arranged by size:
339 240 218 163
SmlI C'TyrA_G
Cuts at: 0 394 960
Size: 394 566
StuI AGG'CCT
Cuts at: 0 382 960
Size: 382 578
StyI C'CwwG_G
Cuts at: 0 434 960
Size: 434 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 907 960
Size: 907 53
XmaI C'CCGG_G
Cuts at: 0 216 555 718 960
Size: 216 339 163 242
Fragments arranged by size:
339 242 216 163
Enzymes that do cut:
AccI AfeI AflIII AhdI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BssHII BstAPI BstBI BstXI BstYI BtgI BtsI EarI
EciI EcoO109I FspI HaeII Hin4I KasI MluI MmeI
MspA1I NarI NspI PmlI PspOMI PstI SacII SapI
SbfI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AleI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BclI BfrBI BglII BlpI BmtI BpmI
BsaI BsaBI BseRI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssSI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI