Restriction map for
pF1KB7632
(Linear) (Six-base) MAPSORT of: KIBB7632.seq Check: 3533 from: 1 to: 1092
KIBB7632.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 406 1092
Size: 406 686
AclI AA'CG_TT
Cuts at: 0 430 692 1092
Size: 430 262 400
Fragments arranged by size:
430 400 262
AflII C'TTAA_G
Cuts at: 0 285 1092
Size: 285 807
AhdI GACnn_n'nnGTC
Cuts at: 0 887 1092
Size: 887 205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 1092
Size: 67 32 993
Fragments arranged by size:
993 67 32
ApaI G_GGCC'C
Cuts at: 0 482 629 1021 1061 1092
Size: 482 147 392 40 31
Fragments arranged by size:
482 392 147 40 31
AvaI C'yCGr_G
Cuts at: 0 577 622 938 1061 1092
Size: 577 45 316 123 31
Fragments arranged by size:
577 316 123 45 31
BanI G'GyrC_C
Cuts at: 0 21 1092
Size: 21 1071
BanII G_rGCy'C
Cuts at: 0 482 629 1021 1043 1061 1092
Size: 482 147 392 22 18 31
Fragments arranged by size:
482 392 147 31 22 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 37 1092
Size: 37 1055
BbvCI CC'TCA_GC
Cuts at: 0 1008 1092
Size: 1008 84
BlpI GC'TnA_GC
Cuts at: 0 413 1092
Size: 413 679
Bme1580I G_kGCm'C
Cuts at: 0 24 482 622 629 1021 1061 1092
Size: 24 458 140 7 392 40 31
Fragments arranged by size:
458 392 140 40 31 24 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1092
Size: 580 512
Bpu10I CC'TnA_GC
Cuts at: 0 1008 1037 1092
Size: 1008 29 55
Fragments arranged by size:
1008 55 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1092
Size: 262 830
BsaAI yAC'GTr
Cuts at: 0 787 1092
Size: 787 305
BsaBI GATnn'nnATC
Cuts at: 0 134 1092
Size: 134 958
BsaWI w'CCGG_w
Cuts at: 0 121 1092
Size: 121 971
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 731 1092
Size: 85 646 361
Fragments arranged by size:
646 361 85
BseYI C'CCAG_C
Cuts at: 0 10 37 748 1092
Size: 10 27 711 344
Fragments arranged by size:
711 344 27 10
Bsp1286I G_dGCh'C
Cuts at: 0 24 482 622 629 1021 1043 1061 1092
Size: 24 458 140 7 392 22 18 31
Fragments arranged by size:
458 392 140 31 24 22 18 7
BspEI T'CCGG_A
Cuts at: 0 121 1092
Size: 121 971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 588 1092
Size: 588 504
BsrBI CCG'CTC
Cuts at: 0 492 573 1092
Size: 492 81 519
Fragments arranged by size:
519 492 81
BsrFI r'CCGG_y
Cuts at: 0 44 1092
Size: 44 1048
BtgI C'CryG_G
Cuts at: 0 987 1092
Size: 987 105
BtsI GCAGTG_nn'
Cuts at: 0 932 1001 1092
Size: 932 69 91
Fragments arranged by size:
932 91 69
EaeI y'GGCC_r
Cuts at: 0 493 1092
Size: 493 599
EarI CTCTTCn'nnn_
Cuts at: 0 221 332 533 680 1092
Size: 221 111 201 147 412
Fragments arranged by size:
412 221 201 147 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1092
Size: 580 512
EcoO109I rG'GnC_Cy
Cuts at: 0 478 479 676 906 1018 1058 1092
Size: 478 1 197 230 112 40 34
Fragments arranged by size:
478 230 197 112 40 34 1
HaeII r_GCGC'y
Cuts at: 0 246 1092
Size: 246 846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 361 393 873 905 1092
Size: 190 32 139 32 480 32 187
Fragments arranged by size:
480 190 187 139 32 32 32
HincII GTy'rAC
Cuts at: 0 303 696 765 1092
Size: 303 393 69 327
Fragments arranged by size:
393 327 303 69
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1092
Size: 367 725
MspA1I CmG'CkG
Cuts at: 0 37 412 1092
Size: 37 375 680
Fragments arranged by size:
680 375 37
NcoI C'CATG_G
Cuts at: 0 987 1092
Size: 987 105
NdeI CA'TA_TG
Cuts at: 0 567 1092
Size: 567 525
PasI CC'CwG_GG
Cuts at: 0 1014 1092
Size: 1014 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 664 1092
Size: 664 428
PfoI T'CCnGG_A
Cuts at: 0 518 1092
Size: 518 574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 1092
Size: 814 32 246
Fragments arranged by size:
814 246 32
PpuMI rG'GwC_Cy
Cuts at: 0 906 1092
Size: 906 186
PspOMI G'GGCC_C
Cuts at: 0 478 625 1017 1057 1092
Size: 478 147 392 40 35
Fragments arranged by size:
478 392 147 40 35
PspXI vC'TCGA_Gb
Cuts at: 0 577 938 1092
Size: 577 361 154
Fragments arranged by size:
577 361 154
PvuII CAG'CTG
Cuts at: 0 412 1092
Size: 412 680
SanDI GG'GwC_CC
Cuts at: 0 906 1092
Size: 906 186
SapI GCTCTTCn'nnn_
Cuts at: 0 221 533 1092
Size: 221 312 559
Fragments arranged by size:
559 312 221
SfcI C'TryA_G
Cuts at: 0 407 1092
Size: 407 685
SmaI CCC'GGG
Cuts at: 0 624 1092
Size: 624 468
SmlI C'TyrA_G
Cuts at: 0 277 285 577 938 1092
Size: 277 8 292 361 154
Fragments arranged by size:
361 292 277 154 8
StuI AGG'CCT
Cuts at: 0 262 291 1092
Size: 262 29 801
Fragments arranged by size:
801 262 29
StyI C'CwwG_G
Cuts at: 0 257 680 837 987 1092
Size: 257 423 157 150 105
Fragments arranged by size:
423 257 157 150 105
TatI w'GTAC_w
Cuts at: 0 974 1092
Size: 974 118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 97 1092
Size: 97 995
XhoI C'TCGA_G
Cuts at: 0 577 938 1092
Size: 577 361 154
Fragments arranged by size:
577 361 154
XmaI C'CCGG_G
Cuts at: 0 622 1092
Size: 622 470
Enzymes that do cut:
AccI AclI AflII AhdI AloI ApaI AvaI BanI
BanII BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaAI BsaBI BsaWI BseRI BseYI Bsp1286I BspEI BspMI
BsrBI BsrFI BtgI BtsI EaeI EarI Eco57MI EcoO109I
HaeII Hin4I HincII MmeI MspA1I NcoI NdeI PasI
PflMI PfoI PpiI PpuMI PspOMI PspXI PvuII SanDI
SapI SfcI SmaI SmlI StuI StyI TatI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BmtI BplI BsaI BsaHI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PshAI PsiI
PsrI PstI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XmnI ZraI