Restriction map for
pF1KB7641
(Linear) (Six-base) MAPSORT of: KIBB7641.seq Check: 8788 from: 1 to: 978
KIBB7641.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 584 668 978
Size: 584 84 310
Fragments arranged by size:
584 310 84
AflIII A'CryG_T
Cuts at: 0 746 978
Size: 746 232
AlwNI CAG_nnn'CTG
Cuts at: 0 204 978
Size: 204 774
ApaI G_GGCC'C
Cuts at: 0 100 361 710 940 978
Size: 100 261 349 230 38
Fragments arranged by size:
349 261 230 100 38
ApaLI G'TGCA_C
Cuts at: 0 157 978
Size: 157 821
AvaI C'yCGr_G
Cuts at: 0 151 522 541 600 703 813 978
Size: 151 371 19 59 103 110 165
Fragments arranged by size:
371 165 151 110 103 59 19
BanI G'GyrC_C
Cuts at: 0 12 59 336 406 432 492 526 665
Size: 12 47 277 70 26 60 34 139
Cuts at: 665 860 978
Size: 195 118
Fragments arranged by size:
277 195 139 118 70 60 47 34
26 12
BanII G_rGCy'C
Cuts at: 0 100 293 361 476 710 940 978
Size: 100 193 68 115 234 230 38
Fragments arranged by size:
234 230 193 115 100 68 38
BbeI G_GCGC'C
Cuts at: 0 436 496 669 864 978
Size: 436 60 173 195 114
Fragments arranged by size:
436 195 173 114 60
BbvCI CC'TCA_GC
Cuts at: 0 422 978
Size: 422 556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 837 871 978
Size: 837 34 107
Fragments arranged by size:
837 107 34
BglI GCCn_nnn'nGGC
Cuts at: 0 418 602 978
Size: 418 184 376
Fragments arranged by size:
418 376 184
Bme1580I G_kGCm'C
Cuts at: 0 17 100 161 361 529 710 940 978
Size: 17 83 61 200 168 181 230 38
Fragments arranged by size:
230 200 181 168 83 61 38 17
BmgBI CAC'GTC
Cuts at: 0 533 978
Size: 533 445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 356 978
Size: 356 622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 978
Size: 304 674
Bpu10I CC'TnA_GC
Cuts at: 0 205 422 978
Size: 205 217 556
Fragments arranged by size:
556 217 205
BsaAI yAC'GTr
Cuts at: 0 873 978
Size: 873 105
BsaHI Gr'CG_yC
Cuts at: 0 433 493 666 861 978
Size: 433 60 173 195 117
Fragments arranged by size:
433 195 173 117 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 978
Size: 160 30 788
Fragments arranged by size:
788 160 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 301 978
Size: 280 21 677
Fragments arranged by size:
677 280 21
BseYI C'CCAG_C
Cuts at: 0 111 475 836 978
Size: 111 364 361 142
Fragments arranged by size:
364 361 142 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 978
Size: 261 717
BsiEI CG_ry'CG
Cuts at: 0 900 978
Size: 900 78
BsiHKAI G_wGCw'C
Cuts at: 0 161 293 659 788 872 978
Size: 161 132 366 129 84 106
Fragments arranged by size:
366 161 132 129 106 84
Bsp1286I G_dGCh'C
Cuts at: 0 17 100 161 293 361 476 529 659
Size: 17 83 61 132 68 115 53 130
Cuts at: 659 710 788 872 940 978
Size: 51 78 84 68 38
Fragments arranged by size:
132 130 115 84 83 78 68 68
61 53 51 38 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 584 668 978
Size: 584 84 310
Fragments arranged by size:
584 310 84
BsrBI CCG'CTC
Cuts at: 0 260 978
Size: 260 718
BsrFI r'CCGG_y
Cuts at: 0 245 429 587 978
Size: 245 184 158 391
Fragments arranged by size:
391 245 184 158
BssHII G'CGCG_C
Cuts at: 0 209 914 978
Size: 209 705 64
Fragments arranged by size:
705 209 64
BstAPI GCAn_nnn'nTGC
Cuts at: 0 792 876 978
Size: 792 84 102
Fragments arranged by size:
792 102 84
Bsu36I CC'TnA_GG
Cuts at: 0 341 402 978
Size: 341 61 576
Fragments arranged by size:
576 341 61
BtgI C'CryG_G
Cuts at: 0 55 100 894 978
Size: 55 45 794 84
Fragments arranged by size:
794 84 55 45
DraIII CAC_nnn'GTG
Cuts at: 0 792 978
Size: 792 186
EaeI y'GGCC_r
Cuts at: 0 897 908 978
Size: 897 11 70
Fragments arranged by size:
897 70 11
EagI C'GGCC_G
Cuts at: 0 897 978
Size: 897 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 978
Size: 58 920
EcoICRI GAG'CTC
Cuts at: 0 291 978
Size: 291 687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 978
Size: 304 674
EcoNI CCTnn'n_nnAGG
Cuts at: 0 283 978
Size: 283 695
EcoO109I rG'GnC_Cy
Cuts at: 0 36 357 358 952 978
Size: 36 321 1 594 26
Fragments arranged by size:
594 321 36 26 1
HaeII r_GCGC'y
Cuts at: 0 46 112 436 496 669 864 978
Size: 46 66 324 60 173 195 114
Fragments arranged by size:
324 195 173 114 66 60 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 978
Size: 307 32 639
Fragments arranged by size:
639 307 32
KasI G'GCGC_C
Cuts at: 0 432 492 665 860 978
Size: 432 60 173 195 118
Fragments arranged by size:
432 195 173 118 60
MluI A'CGCG_T
Cuts at: 0 746 978
Size: 746 232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 978
Size: 158 820
MspA1I CmG'CkG
Cuts at: 0 102 489 907 978
Size: 102 387 418 71
Fragments arranged by size:
418 387 102 71
NaeI GCC'GGC
Cuts at: 0 247 431 978
Size: 247 184 547
Fragments arranged by size:
547 247 184
NarI GG'CG_CC
Cuts at: 0 433 493 666 861 978
Size: 433 60 173 195 117
Fragments arranged by size:
433 195 173 117 60
NgoMIV G'CCGG_C
Cuts at: 0 245 429 978
Size: 245 184 549
Fragments arranged by size:
549 245 184
PasI CC'CwG_GG
Cuts at: 0 361 415 978
Size: 361 54 563
Fragments arranged by size:
563 361 54
PmlI CAC'GTG
Cuts at: 0 873 978
Size: 873 105
PspOMI G'GGCC_C
Cuts at: 0 96 357 706 936 978
Size: 96 261 349 230 42
Fragments arranged by size:
349 261 230 96 42
SacI G_AGCT'C
Cuts at: 0 293 978
Size: 293 685
SacII CC_GC'GG
Cuts at: 0 103 978
Size: 103 875
SexAI A'CCwGG_T
Cuts at: 0 788 978
Size: 788 190
SfoI GGC'GCC
Cuts at: 0 434 494 667 862 978
Size: 434 60 173 195 116
Fragments arranged by size:
434 195 173 116 60
SmaI CCC'GGG
Cuts at: 0 524 705 978
Size: 524 181 273
Fragments arranged by size:
524 273 181
StuI AGG'CCT
Cuts at: 0 232 640 978
Size: 232 408 338
Fragments arranged by size:
408 338 232
StyI C'CwwG_G
Cuts at: 0 164 971 978
Size: 164 807 7
Fragments arranged by size:
807 164 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 978
Size: 225 753
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 978
Size: 40 32 906
Fragments arranged by size:
906 40 32
XmaI C'CCGG_G
Cuts at: 0 522 703 978
Size: 522 181 275
Fragments arranged by size:
522 275 181
Enzymes that do cut:
AarI AflIII AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BbvCI BcgI BglI Bme1580I BmgBI BplI BpmI
Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI Bsu36I
BtgI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MluI MmeI MspA1I NaeI
NarI NgoMIV PasI PmlI PspOMI SacI SacII SexAI
SfoI SmaI StuI StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BpuEI BsaI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EarI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI