(Linear) (Six-base) MAPSORT of: KIBB7648.seq  Check: 4933  from: 1  to: 1176



KIBB7648.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

Acc65I G'GTAC_C

Cuts at:      0    1132    1176 
   Size:       1132      44
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91    1176 
   Size:         91    1085
 
AfeI AGC'GCT

Cuts at:      0     343    1176 
   Size:        343     833
 
AflIII A'CryG_T

Cuts at:      0    1023    1176 
   Size:       1023     153
 
AvaI C'yCGr_G

Cuts at:      0     683    1176 
   Size:        683     493
 
BanI G'GyrC_C

Cuts at:      0    1132    1176 
   Size:       1132      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     249     700    1176 
   Size:        249     451     476

  Fragments arranged by size: 

                 476     451     249
 
BbvCI CC'TCA_GC

Cuts at:      0     104    1176 
   Size:        104    1072
 
BglI GCCn_nnn'nGGC

Cuts at:      0     748    1176 
   Size:        748     428
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     330     835    1176 
   Size:        330     505     341

  Fragments arranged by size: 

                 505     341     330
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1077    1092    1176 
   Size:       1077      15      84

  Fragments arranged by size: 

                1077      84      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     754    1176 
   Size:        571     183     422

  Fragments arranged by size: 

                 571     422     183
 
Bpu10I CC'TnA_GC

Cuts at:      0     104    1176 
   Size:        104    1072
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     544     574    1176 
   Size:        544      30     602

  Fragments arranged by size: 

                 602     544      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97    1084    1176 
   Size:         97     987      92

  Fragments arranged by size: 

                 987      97      92
 
BseYI C'CCAG_C

Cuts at:      0     872    1003    1176 
   Size:        872     131     173

  Fragments arranged by size: 

                 872     173     131
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613    1176 
   Size:        613     563
 
BsmI GAATG_Cn'

Cuts at:      0     335    1176 
   Size:        335     841
 
BspHI T'CATG_A

Cuts at:      0     711    1176 
   Size:        711     465
 
BsrDI GCAATG_nn'

Cuts at:      0     489    1176 
   Size:        489     687
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     757    1176 
   Size:        757     419
 
BstBI TT'CG_AA

Cuts at:      0     330    1176 
   Size:        330     846
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     315     825    1176 
   Size:        315     510     351

  Fragments arranged by size: 

                 510     351     315
 
BstYI r'GATC_y

Cuts at:      0       8    1176 
   Size:          8    1168
 
BtgI C'CryG_G

Cuts at:      0     803    1166    1176 
   Size:        803     363      10

  Fragments arranged by size: 

                 803     363      10
 
BtsI GCAGTG_nn'

Cuts at:      0     260    1103    1176 
   Size:        260     843      73

  Fragments arranged by size: 

                 843     260      73
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     846     881    1176 
   Size:        846      35     295

  Fragments arranged by size: 

                 846     295      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     734    1176 
   Size:        734     442
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      79     221    1176 
   Size:         79     142     955

  Fragments arranged by size: 

                 955     142      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     571     754    1176 
   Size:         91     480     183     422

  Fragments arranged by size: 

                 480     422     183      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0     108    1176 
   Size:        108    1068
 
EcoRV GAT'ATC

Cuts at:      0     597    1176 
   Size:        597     579
 
HaeII r_GCGC'y

Cuts at:      0     345    1176 
   Size:        345     831
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     567     599    1039    1071    1176 
   Size:        567      32     440      32     105

  Fragments arranged by size: 

                 567     440     105      32      32
 
HincII GTy'rAC

Cuts at:      0     672    1176 
   Size:        672     504
 
KpnI G_GTAC'C

Cuts at:      0    1136    1176 
   Size:       1136      40
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     864    1176 
   Size:        864     312
 
MslI CAynn'nnrTG

Cuts at:      0     281    1176 
   Size:        281     895
 
MspA1I CmG'CkG

Cuts at:      0     721    1176 
   Size:        721     455
 
NcoI C'CATG_G

Cuts at:      0    1166    1176 
   Size:       1166      10
 
NdeI CA'TA_TG

Cuts at:      0     666    1176 
   Size:        666     510
 
NspI r_CATG'y

Cuts at:      0     408    1027    1176 
   Size:        408     619     149

  Fragments arranged by size: 

                 619     408     149
 
PciI A'CATG_T

Cuts at:      0    1023    1176 
   Size:       1023     153
 
PfoI T'CCnGG_A

Cuts at:      0     548    1086    1176 
   Size:        548     538      90

  Fragments arranged by size: 

                 548     538      90
 
PpuMI rG'GwC_Cy

Cuts at:      0     108    1176 
   Size:        108    1068
 
PvuII CAG'CTG

Cuts at:      0     721    1176 
   Size:        721     455
 
SapI GCTCTTCn'nnn_

Cuts at:      0     734    1176 
   Size:        734     442
 
StuI AGG'CCT

Cuts at:      0     302    1176 
   Size:        302     874
 
StyI C'CwwG_G

Cuts at:      0    1031    1166    1176 
   Size:       1031     135      10

  Fragments arranged by size: 

                1031     135      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     834    1176 
   Size:        834     342
 
TatI w'GTAC_w

Cuts at:      0     930    1176 
   Size:        930     246
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     243    1176 
   Size:        243     933
 
XmnI GAAnn'nnTTC

Cuts at:      0     946    1176 
   Size:        946     230
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI   AflIII     AvaI     BanI     BbsI    BbvCI
     BglI     BmrI     BplI     BpmI   Bpu10I    BsaXI    BseRI    BseYI
     BsgI     BsmI    BspHI    BsrDI   BstAPI    BstBI    BstXI    BstYI
     BtgI     BtsI    CspCI     EarI     EciI  Eco57MI EcoO109I    EcoRV
    HaeII    Hin4I   HincII     KpnI     MmeI     MslI   MspA1I     NcoI
     NdeI     NspI     PciI     PfoI    PpuMI    PvuII     SapI     StuI
     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     MfeI     MluI     MscI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI