Restriction map for
pF1KB7653
(Linear) (Six-base) MAPSORT of: KIBB7653.seq Check: 227 from: 1 to: 1002
KIBB7653.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 878 1002
Size: 119 759 124
Fragments arranged by size:
759 124 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 828 1002
Size: 828 174
AlwNI CAG_nnn'CTG
Cuts at: 0 522 651 1002
Size: 522 129 351
Fragments arranged by size:
522 351 129
ApoI r'AATT_y
Cuts at: 0 55 601 1002
Size: 55 546 401
Fragments arranged by size:
546 401 55
BanI G'GyrC_C
Cuts at: 0 13 1002
Size: 13 989
BanII G_rGCy'C
Cuts at: 0 895 968 1002
Size: 895 73 34
Fragments arranged by size:
895 73 34
BbeI G_GCGC'C
Cuts at: 0 17 1002
Size: 17 985
BbsI GAAGACnn'nnnn_
Cuts at: 0 855 1002
Size: 855 147
BbvCI CC'TCA_GC
Cuts at: 0 20 1002
Size: 20 982
BglII A'GATC_T
Cuts at: 0 975 1002
Size: 975 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 387 1002
Size: 172 215 615
Fragments arranged by size:
615 215 172
Bpu10I CC'TnA_GC
Cuts at: 0 20 1002
Size: 20 982
BsaHI Gr'CG_yC
Cuts at: 0 14 1002
Size: 14 988
BsaWI w'CCGG_w
Cuts at: 0 887 1002
Size: 887 115
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 452 1002
Size: 37 415 550
Fragments arranged by size:
550 415 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1002
Size: 283 719
BsiHKAI G_wGCw'C
Cuts at: 0 239 629 895 968 1002
Size: 239 390 266 73 34
Fragments arranged by size:
390 266 239 73 34
Bsp1286I G_dGCh'C
Cuts at: 0 239 629 895 968 1002
Size: 239 390 266 73 34
Fragments arranged by size:
390 266 239 73 34
BspEI T'CCGG_A
Cuts at: 0 887 1002
Size: 887 115
BsrDI GCAATG_nn'
Cuts at: 0 926 1002
Size: 926 76
BsrFI r'CCGG_y
Cuts at: 0 32 1002
Size: 32 970
BsrGI T'GTAC_A
Cuts at: 0 358 1002
Size: 358 644
BstAPI GCAn_nnn'nTGC
Cuts at: 0 339 1002
Size: 339 663
BstYI r'GATC_y
Cuts at: 0 78 975 1002
Size: 78 897 27
Fragments arranged by size:
897 78 27
BtsI GCAGTG_nn'
Cuts at: 0 414 1002
Size: 414 588
DraI TTT'AAA
Cuts at: 0 243 1002
Size: 243 759
DrdI GACnn_nn'nnGTC
Cuts at: 0 511 1002
Size: 511 491
EarI CTCTTCn'nnn_
Cuts at: 0 308 1002
Size: 308 694
EcoICRI GAG'CTC
Cuts at: 0 893 966 1002
Size: 893 73 36
Fragments arranged by size:
893 73 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 172 387 878 1002
Size: 119 53 215 491 124
Fragments arranged by size:
491 215 124 119 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 343 1002
Size: 343 659
EcoRI G'AATT_C
Cuts at: 0 601 1002
Size: 601 401
HaeII r_GCGC'y
Cuts at: 0 17 1002
Size: 17 985
HindIII A'AGCT_T
Cuts at: 0 446 1002
Size: 446 556
KasI G'GCGC_C
Cuts at: 0 13 1002
Size: 13 989
MspA1I CmG'CkG
Cuts at: 0 19 219 1002
Size: 19 200 783
Fragments arranged by size:
783 200 19
NaeI GCC'GGC
Cuts at: 0 34 1002
Size: 34 968
NarI GG'CG_CC
Cuts at: 0 14 1002
Size: 14 988
NgoMIV G'CCGG_C
Cuts at: 0 32 1002
Size: 32 970
NspI r_CATG'y
Cuts at: 0 593 1002
Size: 593 409
PflMI CCAn_nnn'nTGG
Cuts at: 0 522 843 1002
Size: 522 321 159
Fragments arranged by size:
522 321 159
PfoI T'CCnGG_A
Cuts at: 0 515 759 968 1002
Size: 515 244 209 34
Fragments arranged by size:
515 244 209 34
PstI C_TGCA'G
Cuts at: 0 344 1002
Size: 344 658
PvuII CAG'CTG
Cuts at: 0 219 1002
Size: 219 783
SacI G_AGCT'C
Cuts at: 0 895 968 1002
Size: 895 73 34
Fragments arranged by size:
895 73 34
SapI GCTCTTCn'nnn_
Cuts at: 0 308 1002
Size: 308 694
SfcI C'TryA_G
Cuts at: 0 340 584 1002
Size: 340 244 418
Fragments arranged by size:
418 340 244
SfoI GGC'GCC
Cuts at: 0 15 1002
Size: 15 987
StuI AGG'CCT
Cuts at: 0 28 1002
Size: 28 974
TatI w'GTAC_w
Cuts at: 0 358 822 1002
Size: 358 464 180
Fragments arranged by size:
464 358 180
XmnI GAAnn'nnTTC
Cuts at: 0 457 1002
Size: 457 545
Enzymes that do cut:
AcuI AlfI AlwNI ApoI BanI BanII BbeI BbsI
BbvCI BglII BpmI Bpu10I BsaHI BsaWI BseRI BsgI
BsiHKAI Bsp1286I BspEI BsrDI BsrFI BsrGI BstAPI BstYI
BtsI DraI DrdI EarI EcoICRI Eco57MI EcoNI EcoRI
HaeII HindIII KasI MspA1I NaeI NarI NgoMIV NspI
PflMI PfoI PstI PvuII SacI SapI SfcI SfoI
StuI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsiEI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII EaeI EagI EciI EcoO109I EcoRV FalI FseI
FspI FspAI Hin4I HincII HpaI KpnI MfeI MluI
MmeI MscI MslI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI