Restriction map for
pF1KB7654
(Linear) (Six-base) MAPSORT of: KIBB7654.seq Check: 6280 from: 1 to: 1113
KIBB7654.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 694 1113
Size: 694 419
Acc65I G'GTAC_C
Cuts at: 0 129 1113
Size: 129 984
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1113
Size: 559 554
AflIII A'CryG_T
Cuts at: 0 456 1113
Size: 456 657
AleI CACnn'nnGTG
Cuts at: 0 876 1113
Size: 876 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 365 1113
Size: 365 748
AlwNI CAG_nnn'CTG
Cuts at: 0 594 686 1113
Size: 594 92 427
Fragments arranged by size:
594 427 92
ApaI G_GGCC'C
Cuts at: 0 99 1113
Size: 99 1014
ApoI r'AATT_y
Cuts at: 0 446 606 1113
Size: 446 160 507
Fragments arranged by size:
507 446 160
BanI G'GyrC_C
Cuts at: 0 75 129 1113
Size: 75 54 984
Fragments arranged by size:
984 75 54
BanII G_rGCy'C
Cuts at: 0 99 1113
Size: 99 1014
BglI GCCn_nnn'nGGC
Cuts at: 0 94 1113
Size: 94 1019
BglII A'GATC_T
Cuts at: 0 484 1113
Size: 484 629
BlpI GC'TnA_GC
Cuts at: 0 770 1113
Size: 770 343
Bme1580I G_kGCm'C
Cuts at: 0 99 804 1113
Size: 99 705 309
Fragments arranged by size:
705 309 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 117 150 803 950 1113
Size: 117 33 653 147 163
Fragments arranged by size:
653 163 147 117 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 622 850 889 994 1113
Size: 616 6 228 39 105 119
Fragments arranged by size:
616 228 119 105 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1113
Size: 476 637
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 1113
Size: 226 30 857
Fragments arranged by size:
857 226 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 195 343 388 709 732 1113
Size: 195 148 45 321 23 381
Fragments arranged by size:
381 321 195 148 45 23
BseYI C'CCAG_C
Cuts at: 0 180 1113
Size: 180 933
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1113
Size: 980 133
BsiHKAI G_wGCw'C
Cuts at: 0 166 1113
Size: 166 947
Bsp1286I G_dGCh'C
Cuts at: 0 99 166 804 1113
Size: 99 67 638 309
Fragments arranged by size:
638 309 99 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 694 738 1113
Size: 41 653 44 375
Fragments arranged by size:
653 375 44 41
BstAPI GCAn_nnn'nTGC
Cuts at: 0 686 942 1113
Size: 686 256 171
Fragments arranged by size:
686 256 171
BstBI TT'CG_AA
Cuts at: 0 474 1113
Size: 474 639
BstEII G'GTnAC_C
Cuts at: 0 22 1113
Size: 22 1091
BstXI CCAn_nnnn'nTGG
Cuts at: 0 107 913 1113
Size: 107 806 200
Fragments arranged by size:
806 200 107
BstYI r'GATC_y
Cuts at: 0 270 484 1113
Size: 270 214 629
Fragments arranged by size:
629 270 214
Bsu36I CC'TnA_GG
Cuts at: 0 947 1113
Size: 947 166
BtgI C'CryG_G
Cuts at: 0 250 644 1113
Size: 250 394 469
Fragments arranged by size:
469 394 250
EaeI y'GGCC_r
Cuts at: 0 979 1113
Size: 979 134
EarI CTCTTCn'nnn_
Cuts at: 0 373 1000 1113
Size: 373 627 113
Fragments arranged by size:
627 373 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 616 622 850 889 994 1113
Size: 559 57 6 228 39 105 119
Fragments arranged by size:
559 228 119 105 57 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 1113
Size: 49 1064
EcoO109I rG'GnC_Cy
Cuts at: 0 95 96 302 816 909 1018 1113
Size: 95 1 206 514 93 109 95
Fragments arranged by size:
514 206 109 95 95 93 1
EcoRV GAT'ATC
Cuts at: 0 659 1113
Size: 659 454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 607 1113
Size: 607 506
HaeII r_GCGC'y
Cuts at: 0 115 1113
Size: 115 998
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 408 440 982 1007 1014 1039 1090 1113
Size: 408 32 542 25 7 25 51 23
Fragments arranged by size:
542 408 51 32 25 25 23 7
KpnI G_GTAC'C
Cuts at: 0 133 1113
Size: 133 980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 863 1113
Size: 233 630 250
Fragments arranged by size:
630 250 233
MscI TGG'CCA
Cuts at: 0 981 1113
Size: 981 132
MslI CAynn'nnrTG
Cuts at: 0 876 1113
Size: 876 237
MspA1I CmG'CkG
Cuts at: 0 769 828 1113
Size: 769 59 285
Fragments arranged by size:
769 285 59
NcoI C'CATG_G
Cuts at: 0 644 1113
Size: 644 469
NdeI CA'TA_TG
Cuts at: 0 323 1113
Size: 323 790
NspI r_CATG'y
Cuts at: 0 945 1113
Size: 945 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 645 882 1113
Size: 567 78 237 231
Fragments arranged by size:
567 237 231 78
PpuMI rG'GwC_Cy
Cuts at: 0 302 816 1018 1113
Size: 302 514 202 95
Fragments arranged by size:
514 302 202 95
PspOMI G'GGCC_C
Cuts at: 0 95 1113
Size: 95 1018
PstI C_TGCA'G
Cuts at: 0 848 1037 1113
Size: 848 189 76
Fragments arranged by size:
848 189 76
PvuII CAG'CTG
Cuts at: 0 769 828 1113
Size: 769 59 285
Fragments arranged by size:
769 285 59
SanDI GG'GwC_CC
Cuts at: 0 302 1113
Size: 302 811
SbfI CC_TGCA'GG
Cuts at: 0 1037 1113
Size: 1037 76
ScaI AGT'ACT
Cuts at: 0 362 1113
Size: 362 751
SfcI C'TryA_G
Cuts at: 0 288 844 1033 1113
Size: 288 556 189 80
Fragments arranged by size:
556 288 189 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 94 1113
Size: 94 1019
SmlI C'TyrA_G
Cuts at: 0 491 1113
Size: 491 622
SpeI A'CTAG_T
Cuts at: 0 702 1113
Size: 702 411
StyI C'CwwG_G
Cuts at: 0 644 1113
Size: 644 469
TatI w'GTAC_w
Cuts at: 0 360 585 737 900 1113
Size: 360 225 152 163 213
Fragments arranged by size:
360 225 213 163 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 226 258 1113
Size: 226 32 855
Fragments arranged by size:
855 226 32
XbaI T'CTAG_A
Cuts at: 0 273 618 1113
Size: 273 345 495
Fragments arranged by size:
495 345 273
XmnI GAAnn'nnTTC
Cuts at: 0 446 1113
Size: 446 667
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
ApoI BanI BanII BglI BglII BlpI Bme1580I BmrI
BpmI BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NcoI NdeI
NspI PflMI PpuMI PspOMI PstI PvuII SanDI SbfI
ScaI SfcI SfiI SmlI SpeI StyI TatI TstI
XbaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SexAI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI StuI SwaI TaqII Tth111I XcmI XhoI
XmaI ZraI