Restriction map for
pF1KB7657
(Linear) (Six-base) MAPSORT of: KIBB7657.seq Check: 8577 from: 1 to: 1002
KIBB7657.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 360 1002
Size: 360 642
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 614 1002
Size: 241 373 388
Fragments arranged by size:
388 373 241
AfeI AGC'GCT
Cuts at: 0 75 1002
Size: 75 927
AgeI A'CCGG_T
Cuts at: 0 55 396 1002
Size: 55 341 606
Fragments arranged by size:
606 341 55
AlwNI CAG_nnn'CTG
Cuts at: 0 682 1002
Size: 682 320
AvaI C'yCGr_G
Cuts at: 0 152 221 667 1002
Size: 152 69 446 335
Fragments arranged by size:
446 335 152 69
BanI G'GyrC_C
Cuts at: 0 269 861 960 1002
Size: 269 592 99 42
Fragments arranged by size:
592 269 99 42
BanII G_rGCy'C
Cuts at: 0 674 1002
Size: 674 328
BbeI G_GCGC'C
Cuts at: 0 273 1002
Size: 273 729
BbsI GAAGACnn'nnnn_
Cuts at: 0 183 488 605 1002
Size: 183 305 117 397
Fragments arranged by size:
397 305 183 117
BglI GCCn_nnn'nGGC
Cuts at: 0 226 1002
Size: 226 776
BglII A'GATC_T
Cuts at: 0 623 1002
Size: 623 379
Bme1580I G_kGCm'C
Cuts at: 0 965 1002
Size: 965 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 181 491 833 988 1002
Size: 90 91 310 342 155 14
Fragments arranged by size:
342 310 155 91 90 14
Bpu10I CC'TnA_GC
Cuts at: 0 460 1002
Size: 460 542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1002
Size: 190 812
BsaI GGTCTCn'nnnn_
Cuts at: 0 865 1002
Size: 865 137
BsaHI Gr'CG_yC
Cuts at: 0 137 270 1002
Size: 137 133 732
Fragments arranged by size:
732 137 133
BsaWI w'CCGG_w
Cuts at: 0 55 396 1002
Size: 55 341 606
Fragments arranged by size:
606 341 55
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 739 883 1002
Size: 739 144 119
Fragments arranged by size:
739 144 119
BseYI C'CCAG_C
Cuts at: 0 288 797 879 931 1002
Size: 288 509 82 52 71
Fragments arranged by size:
509 288 82 71 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 503 729 1002
Size: 394 109 226 273
Fragments arranged by size:
394 273 226 109
BsiHKAI G_wGCw'C
Cuts at: 0 377 674 902 1002
Size: 377 297 228 100
Fragments arranged by size:
377 297 228 100
BsmBI CGTCTCn'nnnn_
Cuts at: 0 766 1002
Size: 766 236
Bsp1286I G_dGCh'C
Cuts at: 0 377 674 902 965 1002
Size: 377 297 228 63 37
Fragments arranged by size:
377 297 228 63 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 360 713 1002
Size: 360 353 289
Fragments arranged by size:
360 353 289
BsrBI CCG'CTC
Cuts at: 0 25 1002
Size: 25 977
BsrFI r'CCGG_y
Cuts at: 0 55 396 1002
Size: 55 341 606
Fragments arranged by size:
606 341 55
BssHII G'CGCG_C
Cuts at: 0 34 1002
Size: 34 968
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 1002
Size: 96 906
BstEII G'GTnAC_C
Cuts at: 0 51 399 1002
Size: 51 348 603
Fragments arranged by size:
603 348 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 1002
Size: 147 855
BstYI r'GATC_y
Cuts at: 0 182 312 623 935 1002
Size: 182 130 311 312 67
Fragments arranged by size:
312 311 182 130 67
BtgI C'CryG_G
Cuts at: 0 389 441 1002
Size: 389 52 561
Fragments arranged by size:
561 389 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 366 1002
Size: 366 636
BtsI GCAGTG_nn'
Cuts at: 0 362 1002
Size: 362 640
EaeI y'GGCC_r
Cuts at: 0 386 657 1002
Size: 386 271 345
Fragments arranged by size:
386 345 271
EcoICRI GAG'CTC
Cuts at: 0 672 1002
Size: 672 330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 181 241 491 614 833 988 1002
Size: 90 91 60 250 123 219 155 14
Fragments arranged by size:
250 219 155 123 91 90 60 14
EcoO109I rG'GnC_Cy
Cuts at: 0 826 1002
Size: 826 176
HaeII r_GCGC'y
Cuts at: 0 77 273 467 1002
Size: 77 196 194 535
Fragments arranged by size:
535 196 194 77
KasI G'GCGC_C
Cuts at: 0 269 1002
Size: 269 733
MslI CAynn'nnrTG
Cuts at: 0 248 1002
Size: 248 754
MspA1I CmG'CkG
Cuts at: 0 682 691 811 931 1002
Size: 682 9 120 120 71
Fragments arranged by size:
682 120 120 71 9
NarI GG'CG_CC
Cuts at: 0 270 1002
Size: 270 732
NdeI CA'TA_TG
Cuts at: 0 764 1002
Size: 764 238
NruI TCG'CGA
Cuts at: 0 80 1002
Size: 80 922
PstI C_TGCA'G
Cuts at: 0 110 834 1002
Size: 110 724 168
Fragments arranged by size:
724 168 110
PvuII CAG'CTG
Cuts at: 0 682 931 1002
Size: 682 249 71
Fragments arranged by size:
682 249 71
SacI G_AGCT'C
Cuts at: 0 674 1002
Size: 674 328
SbfI CC_TGCA'GG
Cuts at: 0 110 1002
Size: 110 892
ScaI AGT'ACT
Cuts at: 0 634 1002
Size: 634 368
SfcI C'TryA_G
Cuts at: 0 106 830 1002
Size: 106 724 172
Fragments arranged by size:
724 172 106
SfoI GGC'GCC
Cuts at: 0 271 1002
Size: 271 731
SmaI CCC'GGG
Cuts at: 0 154 223 1002
Size: 154 69 779
Fragments arranged by size:
779 154 69
SmlI C'TyrA_G
Cuts at: 0 205 1002
Size: 205 797
StyI C'CwwG_G
Cuts at: 0 265 857 919 1002
Size: 265 592 62 83
Fragments arranged by size:
592 265 83 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 549 1002
Size: 549 453
TatI w'GTAC_w
Cuts at: 0 632 1002
Size: 632 370
XmaI C'CCGG_G
Cuts at: 0 152 221 1002
Size: 152 69 781
Fragments arranged by size:
781 152 69
XmnI GAAnn'nnTTC
Cuts at: 0 952 1002
Size: 952 50
Enzymes that do cut:
AarI AcuI AfeI AgeI AlwNI AvaI BanI BanII
BbeI BbsI BglI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI EaeI EcoICRI Eco57MI EcoO109I
HaeII KasI MslI MspA1I NarI NdeI NruI PstI
PvuII SacI SbfI ScaI SfcI SfoI SmaI SmlI
StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaXI
BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NaeI NcoI NgoMIV NheI NotI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI ZraI