(Linear) (Six-base) MAPSORT of: KIBB7660.seq Check: 4687 from: 1 to: 1185 KIBB7660.orf With 182 enzymes: SgfI * June 26, 2008 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 750 1185 Size: 750 435 AccI GT'mk_AC Cuts at: 0 713 1185 Size: 713 472 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1051 1185 Size: 1051 134 AflIII A'CryG_T Cuts at: 0 271 1185 Size: 271 914 AgeI A'CCGG_T Cuts at: 0 598 1185 Size: 598 587 AlwNI CAG_nnn'CTG Cuts at: 0 592 1185 Size: 592 593 ApaI G_GGCC'C Cuts at: 0 64 116 201 442 1160 1178 1185 Size: 64 52 85 241 718 18 7 Fragments arranged by size: 718 241 85 64 52 18 7 ApoI r'AATT_y Cuts at: 0 332 1185 Size: 332 853 AvaI C'yCGr_G Cuts at: 0 620 1137 1185 Size: 620 517 48 Fragments arranged by size: 620 517 48 BamHI G'GATC_C Cuts at: 0 84 1185 Size: 84 1101 BanI G'GyrC_C Cuts at: 0 166 352 406 522 762 1185 Size: 166 186 54 116 240 423 Fragments arranged by size: 423 240 186 166 116 54 BanII G_rGCy'C Cuts at: 0 64 73 116 143 201 305 442 673 Size: 64 9 43 27 58 104 137 231 Cuts at: 673 1160 1178 1185 Size: 487 18 7 Fragments arranged by size: 487 231 137 104 64 58 43 27 18 9 7 BbeI G_GCGC'C Cuts at: 0 356 410 1185 Size: 356 54 775 Fragments arranged by size: 775 356 54 BglI GCCn_nnn'nGGC Cuts at: 0 947 1173 1185 Size: 947 226 12 Fragments arranged by size: 947 226 12 BglII A'GATC_T Cuts at: 0 364 1185 Size: 364 821 BlpI GC'TnA_GC Cuts at: 0 1124 1185 Size: 1124 61 Bme1580I G_kGCm'C Cuts at: 0 64 116 169 201 442 527 616 767 Size: 64 52 53 32 241 85 89 151 Cuts at: 767 1160 1178 1185 Size: 393 18 7 Fragments arranged by size: 393 241 151 89 85 64 53 52 32 18 7 BmgBI CAC'GTC Cuts at: 0 272 1185 Size: 272 913 BmrI ACTGGGnnnn_n' Cuts at: 0 509 517 1185 Size: 509 8 668 Fragments arranged by size: 668 509 8 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 346 728 821 1093 1185 Size: 319 27 382 93 272 92 Fragments arranged by size: 382 319 272 93 92 27 Bpu10I CC'TnA_GC Cuts at: 0 672 1185 Size: 672 513 BsaI GGTCTCn'nnnn_ Cuts at: 0 287 589 1185 Size: 287 302 596 Fragments arranged by size: 596 302 287 BsaHI Gr'CG_yC Cuts at: 0 353 407 1185 Size: 353 54 778 Fragments arranged by size: 778 353 54 BsaWI w'CCGG_w Cuts at: 0 598 934 1185 Size: 598 336 251 Fragments arranged by size: 598 336 251 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 555 1030 1125 1185 Size: 555 475 95 60 Fragments arranged by size: 555 475 95 60 BseYI C'CCAG_C Cuts at: 0 142 526 572 664 784 792 1185 Size: 142 384 46 92 120 8 393 Fragments arranged by size: 393 384 142 120 92 46 8 BsiEI CG_ry'CG Cuts at: 0 264 1185 Size: 264 921 BsiHKAI G_wGCw'C Cuts at: 0 744 1185 Size: 744 441 Bsp1286I G_dGCh'C Cuts at: 0 64 73 116 143 169 201 305 442 Size: 64 9 43 27 26 32 104 137 Cuts at: 442 527 616 673 744 767 1160 1178 1185 Size: 85 89 57 71 23 393 18 7 Fragments arranged by size: 393 137 104 89 85 71 64 57 43 32 27 26 23 18 9 7 BspEI T'CCGG_A Cuts at: 0 934 1185 Size: 934 251 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 750 1185 Size: 750 435 BsrBI CCG'CTC Cuts at: 0 5 491 1059 1185 Size: 5 486 568 126 Fragments arranged by size: 568 486 126 5 BsrFI r'CCGG_y Cuts at: 0 598 1185 Size: 598 587 BssHII G'CGCG_C Cuts at: 0 1095 1185 Size: 1095 90 BstEII G'GTnAC_C Cuts at: 0 904 1185 Size: 904 281 BstXI CCAn_nnnn'nTGG Cuts at: 0 981 1185 Size: 981 204 BstYI r'GATC_y Cuts at: 0 84 364 1040 1185 Size: 84 280 676 145 Fragments arranged by size: 676 280 145 84 Bsu36I CC'TnA_GG Cuts at: 0 345 482 1185 Size: 345 137 703 Fragments arranged by size: 703 345 137 BtgI C'CryG_G Cuts at: 0 47 234 258 974 1147 1185 Size: 47 187 24 716 173 38 Fragments arranged by size: 716 187 173 47 38 24 EaeI y'GGCC_r Cuts at: 0 389 893 1103 1185 Size: 389 504 210 82 Fragments arranged by size: 504 389 210 82 EarI CTCTTCn'nnn_ Cuts at: 0 13 743 1185 Size: 13 730 442 Fragments arranged by size: 730 442 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 332 1069 1185 Size: 332 737 116 Fragments arranged by size: 737 332 116 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 346 728 821 1051 1093 1185 Size: 319 27 382 93 230 42 92 Fragments arranged by size: 382 319 230 93 92 42 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 433 645 1185 Size: 433 212 540 Fragments arranged by size: 540 433 212 EcoO109I rG'GnC_Cy Cuts at: 0 60 61 113 197 198 341 438 439 Size: 60 1 52 84 1 143 97 1 Cuts at: 439 683 1143 1157 1175 1185 Size: 244 460 14 18 10 Fragments arranged by size: 460 244 143 97 84 60 52 18 14 10 1 1 1 EcoRI G'AATT_C Cuts at: 0 332 1185 Size: 332 853 HaeII r_GCGC'y Cuts at: 0 356 410 582 1185 Size: 356 54 172 603 Fragments arranged by size: 603 356 172 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 916 948 1185 Size: 916 32 237 Fragments arranged by size: 916 237 32 KasI G'GCGC_C Cuts at: 0 352 406 1185 Size: 352 54 779 Fragments arranged by size: 779 352 54 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 362 1185 Size: 362 823 MscI TGG'CCA Cuts at: 0 391 895 1105 1185 Size: 391 504 210 80 Fragments arranged by size: 504 391 210 80 MspA1I CmG'CkG Cuts at: 0 260 516 1119 1128 1185 Size: 260 256 603 9 57 Fragments arranged by size: 603 260 256 57 9 NarI GG'CG_CC Cuts at: 0 353 407 1185 Size: 353 54 778 Fragments arranged by size: 778 353 54 NcoI C'CATG_G Cuts at: 0 974 1185 Size: 974 211 NspI r_CATG'y Cuts at: 0 720 1185 Size: 720 465 PasI CC'CwG_GG Cuts at: 0 64 73 1185 Size: 64 9 1112 Fragments arranged by size: 1112 64 9 PflMI CCAn_nnn'nTGG Cuts at: 0 193 240 449 519 1153 1185 Size: 193 47 209 70 634 32 Fragments arranged by size: 634 209 193 70 47 32 PfoI T'CCnGG_A Cuts at: 0 323 474 697 1010 1043 1185 Size: 323 151 223 313 33 142 Fragments arranged by size: 323 313 223 151 142 33 PpuMI rG'GwC_Cy Cuts at: 0 683 1143 1185 Size: 683 460 42 Fragments arranged by size: 683 460 42 PspOMI G'GGCC_C Cuts at: 0 60 112 197 438 1156 1174 1185 Size: 60 52 85 241 718 18 11 Fragments arranged by size: 718 241 85 60 52 18 11 PvuII CAG'CTG Cuts at: 0 516 1119 1128 1185 Size: 516 603 9 57 Fragments arranged by size: 603 516 57 9 RsrII CG'GwC_CG Cuts at: 0 1008 1185 Size: 1008 177 SacII CC_GC'GG Cuts at: 0 261 1185 Size: 261 924 SanDI GG'GwC_CC Cuts at: 0 1143 1185 Size: 1143 42 SfcI C'TryA_G Cuts at: 0 710 1185 Size: 710 475 SfoI GGC'GCC Cuts at: 0 354 408 1185 Size: 354 54 777 Fragments arranged by size: 777 354 54 SmaI CCC'GGG Cuts at: 0 622 1185 Size: 622 563 StuI AGG'CCT Cuts at: 0 428 1185 Size: 428 757 StyI C'CwwG_G Cuts at: 0 536 974 1185 Size: 536 438 211 Fragments arranged by size: 536 438 211 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 727 823 1185 Size: 727 96 362 Fragments arranged by size: 727 362 96 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 384 399 1185 Size: 384 15 786 Fragments arranged by size: 786 384 15 XmaI C'CCGG_G Cuts at: 0 620 1185 Size: 620 565 Enzymes that do cut: AarI AccI AcuI AflIII AgeI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstEII BstXI BstYI Bsu36I BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MmeI MscI MspA1I NarI NcoI NspI PasI PflMI PfoI PpuMI PspOMI PvuII RsrII SacII SanDI SfcI SfoI SmaI StuI StyI TaqII XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BmtI BplI BpuEI BsaAI BsaBI BsaXI BsgI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI