Restriction map for pF1KB7660
 (Linear) (Six-base) MAPSORT of: KIBB7660.seq  Check: 4687  from: 1  to: 1185



KIBB7660.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     750    1185 
   Size:        750     435
 
AccI GT'mk_AC

Cuts at:      0     713    1185 
   Size:        713     472
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1051    1185 
   Size:       1051     134
 
AflIII A'CryG_T

Cuts at:      0     271    1185 
   Size:        271     914
 
AgeI A'CCGG_T

Cuts at:      0     598    1185 
   Size:        598     587
 
AlwNI CAG_nnn'CTG

Cuts at:      0     592    1185 
   Size:        592     593
 
ApaI G_GGCC'C

Cuts at:      0      64     116     201     442    1160    1178    1185 
   Size:         64      52      85     241     718      18       7

  Fragments arranged by size: 

                 718     241      85      64      52      18       7
 
ApoI r'AATT_y

Cuts at:      0     332    1185 
   Size:        332     853
 
AvaI C'yCGr_G

Cuts at:      0     620    1137    1185 
   Size:        620     517      48

  Fragments arranged by size: 

                 620     517      48
 
BamHI G'GATC_C

Cuts at:      0      84    1185 
   Size:         84    1101
 
BanI G'GyrC_C

Cuts at:      0     166     352     406     522     762    1185 
   Size:        166     186      54     116     240     423

  Fragments arranged by size: 

                 423     240     186     166     116      54
 
BanII G_rGCy'C

Cuts at:      0      64      73     116     143     201     305     442     673 
   Size:         64       9      43      27      58     104     137     231

Cuts at:    673    1160    1178    1185 
   Size:        487      18       7

  Fragments arranged by size: 

                 487     231     137     104      64      58      43      27
                  18       9       7
 
BbeI G_GCGC'C

Cuts at:      0     356     410    1185 
   Size:        356      54     775

  Fragments arranged by size: 

                 775     356      54
 
BglI GCCn_nnn'nGGC

Cuts at:      0     947    1173    1185 
   Size:        947     226      12

  Fragments arranged by size: 

                 947     226      12
 
BglII A'GATC_T

Cuts at:      0     364    1185 
   Size:        364     821
 
BlpI GC'TnA_GC

Cuts at:      0    1124    1185 
   Size:       1124      61
 
Bme1580I G_kGCm'C

Cuts at:      0      64     116     169     201     442     527     616     767 
   Size:         64      52      53      32     241      85      89     151

Cuts at:    767    1160    1178    1185 
   Size:        393      18       7

  Fragments arranged by size: 

                 393     241     151      89      85      64      53      52
                  32      18       7
 
BmgBI CAC'GTC

Cuts at:      0     272    1185 
   Size:        272     913
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     509     517    1185 
   Size:        509       8     668

  Fragments arranged by size: 

                 668     509       8
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     346     728     821    1093    1185 
   Size:        319      27     382      93     272      92

  Fragments arranged by size: 

                 382     319     272      93      92      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     672    1185 
   Size:        672     513
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     287     589    1185 
   Size:        287     302     596

  Fragments arranged by size: 

                 596     302     287
 
BsaHI Gr'CG_yC

Cuts at:      0     353     407    1185 
   Size:        353      54     778

  Fragments arranged by size: 

                 778     353      54
 
BsaWI w'CCGG_w

Cuts at:      0     598     934    1185 
   Size:        598     336     251

  Fragments arranged by size: 

                 598     336     251
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     555    1030    1125    1185 
   Size:        555     475      95      60

  Fragments arranged by size: 

                 555     475      95      60
 
BseYI C'CCAG_C

Cuts at:      0     142     526     572     664     784     792    1185 
   Size:        142     384      46      92     120       8     393

  Fragments arranged by size: 

                 393     384     142     120      92      46       8
 
BsiEI CG_ry'CG

Cuts at:      0     264    1185 
   Size:        264     921
 
BsiHKAI G_wGCw'C

Cuts at:      0     744    1185 
   Size:        744     441
 
Bsp1286I G_dGCh'C

Cuts at:      0      64      73     116     143     169     201     305     442 
   Size:         64       9      43      27      26      32     104     137

Cuts at:    442     527     616     673     744     767    1160    1178    1185 
   Size:         85      89      57      71      23     393      18       7

  Fragments arranged by size: 

                 393     137     104      89      85      71      64      57
                  43      32      27      26      23      18       9       7
            
 
BspEI T'CCGG_A

Cuts at:      0     934    1185 
   Size:        934     251
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     750    1185 
   Size:        750     435
 
BsrBI CCG'CTC

Cuts at:      0       5     491    1059    1185 
   Size:          5     486     568     126

  Fragments arranged by size: 

                 568     486     126       5
 
BsrFI r'CCGG_y

Cuts at:      0     598    1185 
   Size:        598     587
 
BssHII G'CGCG_C

Cuts at:      0    1095    1185 
   Size:       1095      90
 
BstEII G'GTnAC_C

Cuts at:      0     904    1185 
   Size:        904     281
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     981    1185 
   Size:        981     204
 
BstYI r'GATC_y

Cuts at:      0      84     364    1040    1185 
   Size:         84     280     676     145

  Fragments arranged by size: 

                 676     280     145      84
 
Bsu36I CC'TnA_GG

Cuts at:      0     345     482    1185 
   Size:        345     137     703

  Fragments arranged by size: 

                 703     345     137
 
BtgI C'CryG_G

Cuts at:      0      47     234     258     974    1147    1185 
   Size:         47     187      24     716     173      38

  Fragments arranged by size: 

                 716     187     173      47      38      24
 
EaeI y'GGCC_r

Cuts at:      0     389     893    1103    1185 
   Size:        389     504     210      82

  Fragments arranged by size: 

                 504     389     210      82
 
EarI CTCTTCn'nnn_

Cuts at:      0      13     743    1185 
   Size:         13     730     442

  Fragments arranged by size: 

                 730     442      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     332    1069    1185 
   Size:        332     737     116

  Fragments arranged by size: 

                 737     332     116
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     346     728     821    1051    1093    1185 
   Size:        319      27     382      93     230      42      92

  Fragments arranged by size: 

                 382     319     230      93      92      42      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     433     645    1185 
   Size:        433     212     540

  Fragments arranged by size: 

                 540     433     212
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60      61     113     197     198     341     438     439 
   Size:         60       1      52      84       1     143      97       1

Cuts at:    439     683    1143    1157    1175    1185 
   Size:        244     460      14      18      10

  Fragments arranged by size: 

                 460     244     143      97      84      60      52      18
                  14      10       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0     332    1185 
   Size:        332     853
 
HaeII r_GCGC'y

Cuts at:      0     356     410     582    1185 
   Size:        356      54     172     603

  Fragments arranged by size: 

                 603     356     172      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     916     948    1185 
   Size:        916      32     237

  Fragments arranged by size: 

                 916     237      32
 
KasI G'GCGC_C

Cuts at:      0     352     406    1185 
   Size:        352      54     779

  Fragments arranged by size: 

                 779     352      54
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     362    1185 
   Size:        362     823
 
MscI TGG'CCA

Cuts at:      0     391     895    1105    1185 
   Size:        391     504     210      80

  Fragments arranged by size: 

                 504     391     210      80
 
MspA1I CmG'CkG

Cuts at:      0     260     516    1119    1128    1185 
   Size:        260     256     603       9      57

  Fragments arranged by size: 

                 603     260     256      57       9
 
NarI GG'CG_CC

Cuts at:      0     353     407    1185 
   Size:        353      54     778

  Fragments arranged by size: 

                 778     353      54
 
NcoI C'CATG_G

Cuts at:      0     974    1185 
   Size:        974     211
 
NspI r_CATG'y

Cuts at:      0     720    1185 
   Size:        720     465
 
PasI CC'CwG_GG

Cuts at:      0      64      73    1185 
   Size:         64       9    1112

  Fragments arranged by size: 

                1112      64       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     193     240     449     519    1153    1185 
   Size:        193      47     209      70     634      32

  Fragments arranged by size: 

                 634     209     193      70      47      32
 
PfoI T'CCnGG_A

Cuts at:      0     323     474     697    1010    1043    1185 
   Size:        323     151     223     313      33     142

  Fragments arranged by size: 

                 323     313     223     151     142      33
 
PpuMI rG'GwC_Cy

Cuts at:      0     683    1143    1185 
   Size:        683     460      42

  Fragments arranged by size: 

                 683     460      42
 
PspOMI G'GGCC_C

Cuts at:      0      60     112     197     438    1156    1174    1185 
   Size:         60      52      85     241     718      18      11

  Fragments arranged by size: 

                 718     241      85      60      52      18      11
 
PvuII CAG'CTG

Cuts at:      0     516    1119    1128    1185 
   Size:        516     603       9      57

  Fragments arranged by size: 

                 603     516      57       9
 
RsrII CG'GwC_CG

Cuts at:      0    1008    1185 
   Size:       1008     177
 
SacII CC_GC'GG

Cuts at:      0     261    1185 
   Size:        261     924
 
SanDI GG'GwC_CC

Cuts at:      0    1143    1185 
   Size:       1143      42
 
SfcI C'TryA_G

Cuts at:      0     710    1185 
   Size:        710     475
 
SfoI GGC'GCC

Cuts at:      0     354     408    1185 
   Size:        354      54     777

  Fragments arranged by size: 

                 777     354      54
 
SmaI CCC'GGG

Cuts at:      0     622    1185 
   Size:        622     563
 
StuI AGG'CCT

Cuts at:      0     428    1185 
   Size:        428     757
 
StyI C'CwwG_G

Cuts at:      0     536     974    1185 
   Size:        536     438     211

  Fragments arranged by size: 

                 536     438     211
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     727     823    1185 
   Size:        727      96     362

  Fragments arranged by size: 

                 727     362      96
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     384     399    1185 
   Size:        384      15     786

  Fragments arranged by size: 

                 786     384      15
 
XmaI C'CCGG_G

Cuts at:      0     620    1185 
   Size:        620     565
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI   BssHII   BstEII    BstXI    BstYI   Bsu36I     BtgI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I
     KasI     MmeI     MscI   MspA1I     NarI     NcoI     NspI     PasI
    PflMI     PfoI    PpuMI   PspOMI    PvuII    RsrII    SacII    SanDI
     SfcI     SfoI     SmaI     StuI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AleI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BspHI
    BsrDI    BsrGI    BssSI   BstAPI    BstBI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MslI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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