(Linear) (Six-base) MAPSORT of: KIBB7667.seq  Check: 7608  from: 1  to: 1011



KIBB7667.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     804    1011 
   Size:        804     207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     561    1011 
   Size:        561     450
 
AfeI AGC'GCT

Cuts at:      0     286    1011 
   Size:        286     725
 
AgeI A'CCGG_T

Cuts at:      0     351     395    1011 
   Size:        351      44     616

  Fragments arranged by size: 

                 616     351      44
 
AhdI GACnn_n'nnGTC

Cuts at:      0     310    1011 
   Size:        310     701
 
AleI CACnn'nnGTG

Cuts at:      0     751    1011 
   Size:        751     260
 
ApoI r'AATT_y

Cuts at:      0     514    1011 
   Size:        514     497
 
BanI G'GyrC_C

Cuts at:      0      66    1011 
   Size:         66     945
 
BanII G_rGCy'C

Cuts at:      0     151    1011 
   Size:        151     860
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     567     950    1011 
   Size:        567     383      61

  Fragments arranged by size: 

                 567     383      61
 
BclI T'GATC_A

Cuts at:      0     407    1011 
   Size:        407     604
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     608    1011 
   Size:        608     403
 
BmtI G_CTAG'C

Cuts at:      0     925    1011 
   Size:        925      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618     734    1011 
   Size:        618     116     277

  Fragments arranged by size: 

                 618     277     116
 
BsaWI w'CCGG_w

Cuts at:      0     351     395    1011 
   Size:        351      44     616

  Fragments arranged by size: 

                 616     351      44
 
BseYI C'CCAG_C

Cuts at:      0     150    1011 
   Size:        150     861
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     729    1011 
   Size:        729     282
 
BsiHKAI G_wGCw'C

Cuts at:      0      77      98    1011 
   Size:         77      21     913

  Fragments arranged by size: 

                 913      77      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      77      98     151    1011 
   Size:         77      21      53     860

  Fragments arranged by size: 

                 860      77      53      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     804    1011 
   Size:        804     207
 
BsrDI GCAATG_nn'

Cuts at:      0     560    1011 
   Size:        560     451
 
BsrFI r'CCGG_y

Cuts at:      0     351     395    1011 
   Size:        351      44     616

  Fragments arranged by size: 

                 616     351      44
 
BsrGI T'GTAC_A

Cuts at:      0     557    1011 
   Size:        557     454
 
BstEII G'GTnAC_C

Cuts at:      0     694    1011 
   Size:        694     317
 
Bsu36I CC'TnA_GG

Cuts at:      0     308    1011 
   Size:        308     703
 
BtsI GCAGTG_nn'

Cuts at:      0     429     471     916    1011 
   Size:        429      42     445      95

  Fragments arranged by size: 

                 445     429      95      42
 
EaeI y'GGCC_r

Cuts at:      0     338    1011 
   Size:        338     673
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     561     618     734    1011 
   Size:        561      57     116     277

  Fragments arranged by size: 

                 561     277     116      57
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     243     279    1011 
   Size:        243      36     732

  Fragments arranged by size: 

                 732     243      36
 
HaeII r_GCGC'y

Cuts at:      0     288    1011 
   Size:        288     723
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     296     328     862     894    1011 
   Size:        296      32     534      32     117

  Fragments arranged by size: 

                 534     296     117      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     530    1011 
   Size:        530     481
 
MscI TGG'CCA

Cuts at:      0     340    1011 
   Size:        340     671
 
MslI CAynn'nnrTG

Cuts at:      0     193     751    1011 
   Size:        193     558     260

  Fragments arranged by size: 

                 558     260     193
 
MspA1I CmG'CkG

Cuts at:      0     422    1011 
   Size:        422     589
 
NheI G'CTAG_C

Cuts at:      0     921    1011 
   Size:        921      90
 
NspI r_CATG'y

Cuts at:      0     666    1011 
   Size:        666     345
 
PshAI GACnn'nnGTC

Cuts at:      0     398    1011 
   Size:        398     613
 
StuI AGG'CCT

Cuts at:      0     739    1011 
   Size:        739     272
 
StyI C'CwwG_G

Cuts at:      0     478     789    1011 
   Size:        478     311     222

  Fragments arranged by size: 

                 478     311     222
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      54    1011 
   Size:         54     957
 
TatI w'GTAC_w

Cuts at:      0     557    1011 
   Size:        557     454
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     817    1011 
   Size:        817     194
 
XmnI GAAnn'nnTTC

Cuts at:      0     835    1011 
   Size:        835     176
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AgeI     AhdI     AleI     ApoI     BanI
    BanII     BbsI     BclI     BmrI     BmtI     BpmI    BsaWI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI   BstEII
   Bsu36I     BtsI     EaeI  Eco57MI     FalI    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I     NheI     NspI    PshAI     StuI     StyI
    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AlfI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI