Restriction map for
pF1KB7668
(Linear) (Six-base) MAPSORT of: KIBB7668.seq Check: 1870 from: 1 to: 1206
KIBB7668.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 280 1206
Size: 280 926
AfeI AGC'GCT
Cuts at: 0 974 1206
Size: 974 232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 679 711 1206
Size: 679 32 495
Fragments arranged by size:
679 495 32
ApaI G_GGCC'C
Cuts at: 0 1118 1206
Size: 1118 88
ApaLI G'TGCA_C
Cuts at: 0 7 1206
Size: 7 1199
AvaI C'yCGr_G
Cuts at: 0 525 1057 1180 1206
Size: 525 532 123 26
Fragments arranged by size:
532 525 123 26
AvrII C'CTAG_G
Cuts at: 0 729 1206
Size: 729 477
BanI G'GyrC_C
Cuts at: 0 529 733 1141 1206
Size: 529 204 408 65
Fragments arranged by size:
529 408 204 65
BanII G_rGCy'C
Cuts at: 0 314 364 1057 1118 1187 1206
Size: 314 50 693 61 69 19
Fragments arranged by size:
693 314 69 61 50 19
BbeI G_GCGC'C
Cuts at: 0 737 1206
Size: 737 469
BglI GCCn_nnn'nGGC
Cuts at: 0 1073 1206
Size: 1073 133
BlpI GC'TnA_GC
Cuts at: 0 721 1206
Size: 721 485
Bme1580I G_kGCm'C
Cuts at: 0 11 532 1078 1118 1206
Size: 11 521 546 40 88
Fragments arranged by size:
546 521 88 40 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 540 1206
Size: 540 666
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 595 912 1206
Size: 418 177 317 294
Fragments arranged by size:
418 317 294 177
Bpu10I CC'TnA_GC
Cuts at: 0 358 1206
Size: 358 848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 568 1206
Size: 358 210 638
Fragments arranged by size:
638 358 210
BsaI GGTCTCn'nnnn_
Cuts at: 0 640 1206
Size: 640 566
BsaHI Gr'CG_yC
Cuts at: 0 734 961 1206
Size: 734 227 245
Fragments arranged by size:
734 245 227
BsaWI w'CCGG_w
Cuts at: 0 755 1206
Size: 755 451
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 322 529 1206
Size: 322 207 677
Fragments arranged by size:
677 322 207
BseYI C'CCAG_C
Cuts at: 0 217 780 966 1117 1206
Size: 217 563 186 151 89
Fragments arranged by size:
563 217 186 151 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1206
Size: 1066 140
BsiEI CG_ry'CG
Cuts at: 0 42 789 923 1206
Size: 42 747 134 283
Fragments arranged by size:
747 283 134 42
BsiHKAI G_wGCw'C
Cuts at: 0 11 314 364 1187 1206
Size: 11 303 50 823 19
Fragments arranged by size:
823 303 50 19 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 487 571 1206
Size: 457 30 84 635
Fragments arranged by size:
635 457 84 30
Bsp1286I G_dGCh'C
Cuts at: 0 11 314 364 532 1057 1078 1118 1187
Size: 11 303 50 168 525 21 40 69
Cuts at: 1187 1206
Size: 19
Fragments arranged by size:
525 303 168 69 50 40 21 19
11
BspEI T'CCGG_A
Cuts at: 0 755 1206
Size: 755 451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 280 1206
Size: 280 926
BsrBI CCG'CTC
Cuts at: 0 27 987 1139 1206
Size: 27 960 152 67
Fragments arranged by size:
960 152 67 27
BsrFI r'CCGG_y
Cuts at: 0 16 193 301 649 991 1206
Size: 16 177 108 348 342 215
Fragments arranged by size:
348 342 215 177 108 16
BssHII G'CGCG_C
Cuts at: 0 200 1206
Size: 200 1006
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 1089 1206
Size: 188 901 117
Fragments arranged by size:
901 188 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 573 936 1206
Size: 225 348 363 270
Fragments arranged by size:
363 348 270 225
BtgI C'CryG_G
Cuts at: 0 763 1004 1206
Size: 763 241 202
Fragments arranged by size:
763 241 202
BtsI GCAGTG_nn'
Cuts at: 0 9 442 1206
Size: 9 433 764
Fragments arranged by size:
764 433 9
EaeI y'GGCC_r
Cuts at: 0 497 786 920 1206
Size: 497 289 134 286
Fragments arranged by size:
497 289 286 134
EagI C'GGCC_G
Cuts at: 0 786 920 1206
Size: 786 134 286
Fragments arranged by size:
786 286 134
EcoICRI GAG'CTC
Cuts at: 0 312 362 1185 1206
Size: 312 50 823 21
Fragments arranged by size:
823 312 50 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 595 912 1206
Size: 418 177 317 294
Fragments arranged by size:
418 317 294 177
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 1206
Size: 376 830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 1206
Size: 609 597
FspI TGC'GCA
Cuts at: 0 232 1206
Size: 232 974
HaeII r_GCGC'y
Cuts at: 0 177 728 737 976 1206
Size: 177 551 9 239 230
Fragments arranged by size:
551 239 230 177 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1018 1050 1206
Size: 1018 32 156
Fragments arranged by size:
1018 156 32
KasI G'GCGC_C
Cuts at: 0 733 1206
Size: 733 473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1206
Size: 464 742
MslI CAynn'nnrTG
Cuts at: 0 274 673 1027 1206
Size: 274 399 354 179
Fragments arranged by size:
399 354 274 179
MspA1I CmG'CkG
Cuts at: 0 294 573 636 720 765 780 966 1006
Size: 294 279 63 84 45 15 186 40
Cuts at: 1006 1109 1148 1157 1206
Size: 103 39 9 49
Fragments arranged by size:
294 279 186 103 84 63 49 45
40 39 15 9
NaeI GCC'GGC
Cuts at: 0 195 993 1206
Size: 195 798 213
Fragments arranged by size:
798 213 195
NarI GG'CG_CC
Cuts at: 0 734 1206
Size: 734 472
NgoMIV G'CCGG_C
Cuts at: 0 193 991 1206
Size: 193 798 215
Fragments arranged by size:
798 215 193
NotI GC'GGCC_GC
Cuts at: 0 786 1206
Size: 786 420
NspI r_CATG'y
Cuts at: 0 663 774 1206
Size: 663 111 432
Fragments arranged by size:
663 432 111
PfoI T'CCnGG_A
Cuts at: 0 434 1206
Size: 434 772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 971 1003 1206
Size: 319 32 620 32 203
Fragments arranged by size:
620 319 203 32 32
PshAI GACnn'nnGTC
Cuts at: 0 1164 1206
Size: 1164 42
PspOMI G'GGCC_C
Cuts at: 0 1114 1206
Size: 1114 92
PstI C_TGCA'G
Cuts at: 0 449 1206
Size: 449 757
PvuII CAG'CTG
Cuts at: 0 294 573 636 720 1206
Size: 294 279 63 84 486
Fragments arranged by size:
486 294 279 84 63
RsrII CG'GwC_CG
Cuts at: 0 411 1206
Size: 411 795
SacI G_AGCT'C
Cuts at: 0 314 364 1187 1206
Size: 314 50 823 19
Fragments arranged by size:
823 314 50 19
SacII CC_GC'GG
Cuts at: 0 766 1007 1206
Size: 766 241 199
Fragments arranged by size:
766 241 199
SexAI A'CCwGG_T
Cuts at: 0 224 1206
Size: 224 982
SfcI C'TryA_G
Cuts at: 0 445 1152 1206
Size: 445 707 54
Fragments arranged by size:
707 445 54
SfoI GGC'GCC
Cuts at: 0 735 1206
Size: 735 471
SgrAI Cr'CCGG_yG
Cuts at: 0 301 1206
Size: 301 905
SmlI C'TyrA_G
Cuts at: 0 373 583 1206
Size: 373 210 623
Fragments arranged by size:
623 373 210
SphI G_CATG'C
Cuts at: 0 774 1206
Size: 774 432
StuI AGG'CCT
Cuts at: 0 1067 1206
Size: 1067 139
StyI C'CwwG_G
Cuts at: 0 729 846 1206
Size: 729 117 360
Fragments arranged by size:
729 360 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 1206
Size: 55 32 1119
Fragments arranged by size:
1119 55 32
Tth111I GACn'n_nGTC
Cuts at: 0 480 1206
Size: 480 726
XmnI GAAnn'nnTTC
Cuts at: 0 127 334 1206
Size: 127 207 872
Fragments arranged by size:
872 207 127
Enzymes that do cut:
AarI AfeI AloI ApaI ApaLI AvaI AvrII BanI
BanII BbeI BglI BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstXI BtgI BtsI EaeI EagI EcoICRI Eco57MI
EcoNI FalI FspI HaeII Hin4I KasI MmeI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PfoI PpiI
PshAI PspOMI PstI PvuII RsrII SacI SacII SexAI
SfcI SfoI SgrAI SmlI SphI StuI StyI TstI
Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApoI AscI AseI AsiSI
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BsaAI BsaBI BsaXI BsiWI
BsmI BspHI BsrDI BsrGI BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoO109I EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI
NheI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpuMI PsiI PspXI PsrI PvuI SalI SanDI
SapI SbfI ScaI SfiI SgfI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII TatI XbaI XcmI XhoI
XmaI ZraI