Restriction map for
pF1KB7676
(Linear) (Six-base) MAPSORT of: KIBB7676.seq Check: 7734 from: 1 to: 1218
KIBB7676.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 0 1138 1218
Size: 1138 80
AccI GT'mk_AC
Cuts at: 0 488 1006 1218
Size: 488 518 212
Fragments arranged by size:
518 488 212
AfeI AGC'GCT
Cuts at: 0 340 1218
Size: 340 878
AflIII A'CryG_T
Cuts at: 0 433 1218
Size: 433 785
AleI CACnn'nnGTG
Cuts at: 0 994 1218
Size: 994 224
ApaI G_GGCC'C
Cuts at: 0 25 697 1045 1218
Size: 25 672 348 173
Fragments arranged by size:
672 348 173 25
ApaLI G'TGCA_C
Cuts at: 0 31 426 1218
Size: 31 395 792
Fragments arranged by size:
792 395 31
AvaI C'yCGr_G
Cuts at: 0 25 392 690 780 950 1218
Size: 25 367 298 90 170 268
Fragments arranged by size:
367 298 268 170 90 25
BanII G_rGCy'C
Cuts at: 0 25 272 691 697 1045 1058 1218
Size: 25 247 419 6 348 13 160
Fragments arranged by size:
419 348 247 160 25 13 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 622 799 1218
Size: 317 305 177 419
Fragments arranged by size:
419 317 305 177
BglI GCCn_nnn'nGGC
Cuts at: 0 359 556 583 1218
Size: 359 197 27 635
Fragments arranged by size:
635 359 197 27
BglII A'GATC_T
Cuts at: 0 176 1218
Size: 176 1042
BlpI GC'TnA_GC
Cuts at: 0 730 1072 1218
Size: 730 342 146
Fragments arranged by size:
730 342 146
Bme1580I G_kGCm'C
Cuts at: 0 25 35 430 655 697 1045 1218
Size: 25 10 395 225 42 348 173
Fragments arranged by size:
395 348 225 173 42 25 10
BmgBI CAC'GTC
Cuts at: 0 555 812 1218
Size: 555 257 406
Fragments arranged by size:
555 406 257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 92 1218
Size: 92 1126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 913 1065 1186 1218
Size: 280 633 152 121 32
Fragments arranged by size:
633 280 152 121 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1218
Size: 217 1001
BsaAI yAC'GTr
Cuts at: 0 425 434 1218
Size: 425 9 784
Fragments arranged by size:
784 425 9
BsaHI Gr'CG_yC
Cuts at: 0 875 1033 1135 1218
Size: 875 158 102 83
Fragments arranged by size:
875 158 102 83
BseYI C'CCAG_C
Cuts at: 0 600 1218
Size: 600 618
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 679 1218
Size: 355 324 539
Fragments arranged by size:
539 355 324
BsiEI CG_ry'CG
Cuts at: 0 102 569 673 1218
Size: 102 467 104 545
Fragments arranged by size:
545 467 104 102
BsiHKAI G_wGCw'C
Cuts at: 0 35 272 430 758 1058 1218
Size: 35 237 158 328 300 160
Fragments arranged by size:
328 300 237 160 158 35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 109 1218
Size: 109 1109
Bsp1286I G_dGCh'C
Cuts at: 0 25 35 272 430 655 691 697 758
Size: 25 10 237 158 225 36 6 61
Cuts at: 758 1045 1058 1218
Size: 287 13 160
Fragments arranged by size:
287 237 225 160 158 61 36 25
13 10 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 1218
Size: 203 1015
BsrFI r'CCGG_y
Cuts at: 0 160 1218
Size: 160 1058
BssHII G'CGCG_C
Cuts at: 0 387 1218
Size: 387 831
BstAPI GCAn_nnn'nTGC
Cuts at: 0 302 1218
Size: 302 916
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 1218
Size: 624 594
BstYI r'GATC_y
Cuts at: 0 176 705 1218
Size: 176 529 513
Fragments arranged by size:
529 513 176
BtgI C'CryG_G
Cuts at: 0 102 452 578 1218
Size: 102 350 126 640
Fragments arranged by size:
640 350 126 102
BtsI GCAGTG_nn'
Cuts at: 0 144 220 1218
Size: 144 76 998
Fragments arranged by size:
998 144 76
DraIII CAC_nnn'GTG
Cuts at: 0 434 1218
Size: 434 784
DrdI GACnn_nn'nnGTC
Cuts at: 0 126 484 843 1218
Size: 126 358 359 375
Fragments arranged by size:
375 359 358 126
EaeI y'GGCC_r
Cuts at: 0 566 584 670 1218
Size: 566 18 86 548
Fragments arranged by size:
566 548 86 18
EagI C'GGCC_G
Cuts at: 0 566 670 1218
Size: 566 104 548
Fragments arranged by size:
566 548 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 81 1218
Size: 81 1137
EcoICRI GAG'CTC
Cuts at: 0 270 1056 1218
Size: 270 786 162
Fragments arranged by size:
786 270 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 913 1065 1186 1218
Size: 280 633 152 121 32
Fragments arranged by size:
633 280 152 121 32
EcoO109I rG'GnC_Cy
Cuts at: 0 22 694 924 1218
Size: 22 672 230 294
Fragments arranged by size:
672 294 230 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 867 1218
Size: 867 351
FspI TGC'GCA
Cuts at: 0 808 1218
Size: 808 410
FspAI rTGC'GCAy
Cuts at: 0 808 1218
Size: 808 410
HaeII r_GCGC'y
Cuts at: 0 327 342 514 1021 1218
Size: 327 15 172 507 197
Fragments arranged by size:
507 327 197 172 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 375 407 1218
Size: 375 32 811
Fragments arranged by size:
811 375 32
HincII GTy'rAC
Cuts at: 0 1007 1218
Size: 1007 211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1085 1218
Size: 245 840 133
Fragments arranged by size:
840 245 133
MslI CAynn'nnrTG
Cuts at: 0 994 1218
Size: 994 224
MspA1I CmG'CkG
Cuts at: 0 104 350 562 571 580 1218
Size: 104 246 212 9 9 638
Fragments arranged by size:
638 246 212 104 9 9
NaeI GCC'GGC
Cuts at: 0 162 1218
Size: 162 1056
NgoMIV G'CCGG_C
Cuts at: 0 160 1218
Size: 160 1058
NotI GC'GGCC_GC
Cuts at: 0 566 1218
Size: 566 652
NspI r_CATG'y
Cuts at: 0 225 807 1218
Size: 225 582 411
Fragments arranged by size:
582 411 225
PmlI CAC'GTG
Cuts at: 0 425 434 1218
Size: 425 9 784
Fragments arranged by size:
784 425 9
PpuMI rG'GwC_Cy
Cuts at: 0 924 1218
Size: 924 294
PshAI GACnn'nnGTC
Cuts at: 0 1138 1218
Size: 1138 80
PspOMI G'GGCC_C
Cuts at: 0 21 693 1041 1218
Size: 21 672 348 177
Fragments arranged by size:
672 348 177 21
PstI C_TGCA'G
Cuts at: 0 521 576 1025 1218
Size: 521 55 449 193
Fragments arranged by size:
521 449 193 55
RsrII CG'GwC_CG
Cuts at: 0 183 1218
Size: 183 1035
SacI G_AGCT'C
Cuts at: 0 272 1058 1218
Size: 272 786 160
Fragments arranged by size:
786 272 160
SacII CC_GC'GG
Cuts at: 0 105 581 1218
Size: 105 476 637
Fragments arranged by size:
637 476 105
SalI G'TCGA_C
Cuts at: 0 1005 1218
Size: 1005 213
SfcI C'TryA_G
Cuts at: 0 318 517 572 1021 1218
Size: 318 199 55 449 197
Fragments arranged by size:
449 318 199 197 55
SmaI CCC'GGG
Cuts at: 0 692 1218
Size: 692 526
SmlI C'TyrA_G
Cuts at: 0 232 1218
Size: 232 986
SphI G_CATG'C
Cuts at: 0 807 1218
Size: 807 411
SrfI GCCC'GGGC
Cuts at: 0 692 1218
Size: 692 526
StyI C'CwwG_G
Cuts at: 0 878 1218
Size: 878 340
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1218
Size: 199 32 987
Fragments arranged by size:
987 199 32
XmaI C'CCGG_G
Cuts at: 0 690 1218
Size: 690 528
ZraI GAC'GTC
Cuts at: 0 1136 1218
Size: 1136 82
Enzymes that do cut:
AatII AccI AfeI AflIII AleI ApaI ApaLI AvaI
BanII BbsI BglI BglII BlpI Bme1580I BmgBI BplI
BpmI BpuEI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BssHII BstAPI BstXI BstYI
BtgI BtsI DraIII DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I HincII
MmeI MslI MspA1I NaeI NgoMIV NotI NspI PmlI
PpuMI PshAI PspOMI PstI RsrII SacI SacII SalI
SfcI SmaI SmlI SphI SrfI StyI TstI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AgeI AhdI AlfI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BmrI BmtI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EarI EcoNI EcoRI EcoRV FseI HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NcoI NdeI NheI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PpiI PsiI PspXI PsrI PvuI PvuII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SspI StuI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmnI